Browse > Article
http://dx.doi.org/10.3347/kjp.2015.53.1.85

Proteomic Screening of Antigenic Proteins from the Hard Tick, Haemaphysalis longicornis (Acari: Ixodidae)  

Kim, Young-Ha (Department of Veterinary Parasitology, College of Veterinary Medicine and Biosafety Research Centre, Chonbuk National University)
Islam, Mohammad Saiful (Department of Veterinary Parasitology, College of Veterinary Medicine and Biosafety Research Centre, Chonbuk National University)
You, Myung-Jo (Department of Veterinary Parasitology, College of Veterinary Medicine and Biosafety Research Centre, Chonbuk National University)
Publication Information
Parasites, Hosts and Diseases / v.53, no.1, 2015 , pp. 85-93 More about this Journal
Abstract
Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional electrophoresis (2-DE) immunoblotting was used for whole body proteins from unfed and partially fed female ticks. Reactivity bands and 2-DE immunoblotting were performed following 2-DE electrophoresis to identify protein spots. The proteome of the partially fed female had a larger number of lower molecular weight proteins than that of the unfed female tick. The total number of detected spots was 818 for unfed and 670 for partially fed female ticks. The 2-DE immunoblotting identified 10 antigenic spots from unfed females and 8 antigenic spots from partially fed females. Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF) of relevant spots identified calreticulin, putative secreted WC salivary protein, and a conserved hypothetical protein from the National Center for Biotechnology Information and Swiss Prot protein sequence databases. These findings indicate that most of the whole body components of these ticks are non-immunogenic. The data reported here will provide guidance in the identification of antigenic proteins to prevent infestation and diseases transmitted by H. longicornis.
Keywords
Haemaphysalis longicornis; tick; antigenic protein; proteomic approach;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Wang H, Kaufman WR, Cui WW, Nuttall PA. Molecular individuality and adaptation of the tick Rhipicephalus appendiculatus in changed feeding environments. Med Vet Entomol 2001; 15: 403-412.   DOI
2 Xu G, Fang QQ, Keirans JE, Durden LA. Cloning and sequencing of putative calreticulin complementary DNAs from four hard tick species. J Parasitol 2004; 90: 73-78.   DOI
3 Imamura S, da Silva Vaz I Jr, Konnai S, Yamada S, Nakajima C, Onuma M, Ohashi K. Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis. Exp Appl Acarol 2009; 48: 345-358.   DOI
4 You M, Fujisaki K. Vaccination effects of recombinant chitinase protein from the hard tick Haemaphysalis longicornis (Acari: Ixodidae). J Vet Med Sci 2009; 71: 709-712.   DOI
5 You M, Xuan X, Tsuji N, Kamio T, Igarashi I, Nagasawa H, Mikami T, Fujisaki K. Molecular characterization of a troponin I-like protein from the hard tick Haemaphysalis longicornis. Insect Biochem Mol Biol 2001; 32: 67-73.   DOI
6 Trager W. Acquired immunity to ticks. J Parasitol 1939; 25: 57-81.   DOI
7 Wikel SK. Host immunity to ticks. Ann Rev Entomol 1996; 41: 1-22.   DOI
8 Allen J R, Humphreys SJ. Immunisation of guinea pigs and cattle against tick. Nature 1979; 280: 491-493.   DOI
9 Rand KN, Moore T, Sriskantha A, Spring K, Tellam R, Willadsen P, Cobon GS. Cloning and expression of a protective antigen from the cattle tick Boophilus microplus. Proc Natl Acad Sci USA 1989; 86: 9657-9661.   DOI
10 Paesen GC, Adams PL, Harlos K, Nuttall PA, Stuart DI. Tick histamine-binding proteins: isolation, cloning, and three-dimensional structure. Mol Cell 1999; 3: 661-671.   DOI
11 You MJ. Immunization of mice with recombinant P27/30 protein confers protection against hard tick Haemaphysalis longicornis (Acari: Ixodidae) infestation. J Vet Sci 2005; 6: 47-51.
12 Nuttall PA, Trimnell AR, Kazimirova M, Labuda M. Exposed and concealed antigens as vaccine targets for controlling ticks and tick-borne diseases. Parasite Immunol 2006; 28: 155-163.   DOI
13 Imamura S, da Silva Vaz I Jr, Sugino M, Ohashi K, Onuma M. A serine protease inhibitor (serpin) from Haemaphysalis longicornis as an anti-tick vaccine. Vaccine 2005; 23: 1301-1311.   DOI
14 O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975; 250: 4007-4021.
15 Bjellqvist B, Ek K, Righetti PG, Gianazza E, Gorg A, Westermeier R, Postel W. Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications. J Biochem Biophys Methods 1982; 6: 317-339.   DOI
16 Kongsuwan K, Josh P, Zhu Y, Pearson R, Gough J, Colgrave ML. Exploring the midgut proteome of partially fed female cattle tick (Rhipicephalus (Boophilus) microplus). J Insect Physiol 2010; 56: 212-226.   DOI
17 Gevaert K, Vandekerckhove J. Protein identification methods in proteomics. Electrophoresis 2000; 21: 1145-1154.   DOI
18 Valenzuela JG, Francischetti IM, Pham VM, Garfield MK, Mather TN, Ribeiro JM. Exploring the sialome of the tick Ixodes scapularis. J Exp Biol 2002; 205: 2843-2864.
19 Madden RD, Sauer JR, Dillwith JW. A proteomics approach to characterizing tick salivary secretions. Exp App Acarol 2002; 28: 77-87.   DOI
20 Mans BJ, Venter JD, Vrey PJ, Louw AI, Neitz AW. Identification of putative proteins involved in granule biogenesis of tick salivary glands. Electrophoresis 2001; 22: 1739-1746.   DOI
21 Oleaga A, Escudero-Poblacion A, Camafeita E, Perez-Sanchez R. A proteomic approach to the identification of salivary proteins from the argasid ticks Ornithodoros moubata and Ornithodoros erraticus. Insect Biochem Mol Biol 2007; 37: 1149-1159.   DOI
22 Rachinsky A, Guerrero FD, Scoles GA. Differential protein expression in ovaries of uninfected and Babesia-infected southern cattle ticks, Rhipicephalus (Boophilus) microplus. Insect Biochem Mol Biol 2007; 37: 1291-1308.   DOI
23 Untalan PM, Guerrero FD, Haines LR, Pearson TW. Proteome analysis of abundantly expressed proteins from unfed larvae of the cattle tick, Boophilus microplus. Insect Biochem Mol Biol 2005; 35: 141-151.   DOI
24 Gao J, Luo J, Fan R, Fingerle V, Guan G, Liu Z, Li Y, Zhao H, Ma M, Liu J, Liu A, Ren Q, Dang Z, Sugimoto C, Yin H. Cloning and characterization of a cDNA clone encoding calreticulin from Haemaphysalis qinghaiensis (Acari: Ixodidae). Parasitol Res 2008; 102: 737-746.   DOI
25 Vennestrom J, Jensen PM. Ixodes ricinus: the potential of two-dimensional gel electrophoresis as a tool for studying host-vector-pathogen interactions. Exp Parasitol 2007; 115: 53-58.   DOI
26 Shin GW, Palaksha KJ, Yang HH, Shin YS, Kim YR, Lee EY, Oh MJ, Jung TS. Partial two-dimensional gel electrophoresis (2-DE) maps of Streptococcus iniae ATCC29178 and Lactococcus garvieae KG9408. Dis Aquat Organ 2006; 70: 71-79.   DOI
27 Ferreira CA, da Silva Vaz I, da Silva SS, Haag KL, Valenzuela JG, Masuda A. Cloning and partial characterization of a Boophilus microplus (Acari: Ixodidae) calreticulin. Exp Parasitol 2002; 101: 25-34.   DOI
28 Jaworski DC, Simmen FA, Lamoreaux W, Coons LB, Muller MT, Needham GR. A secreted calreticulin protein in ixodid tick (Amblyomma americanum) saliva. J Insect Physiol 1995; 41: 369-375.   DOI
29 Parizi LF, Rech H, Ferreira CA, Imamura S, Ohashi K, Onuma M, Masuda A, da Silva Vaz I Jr. Comparative immunogenicity of Haemaphysalis longicornis and Rhipicephalus (Boophilus) microplus calreticulins. Vet Parasitol 2009; 164: 282-290.   DOI
30 Sanders ML, Jaworski DC, Sanchez JL, DeFraites RE, Glass GE, Scott AL, Raha S, Ritchie BC, Needham GR, Schwartz BS. Antibody to a cDNA-derived calreticulin protein from Amblyomma americanum as a biomarker of tick exposure in humans. Am J Trop Med Hyg 1998; 59: 279-285.   DOI
31 Coppolino MG, Dedhar S. Calreticulin. Int J Biochem Cell Biol 1998; 30: 553-558.   DOI
32 Hackman RH. The soluble cuticular proteins form three arthropod species: Scylla serrata (Decapoda: Portunidae), Boophilus microplus (Acarina: Ixodidae) and Agrianome spinicollis (Coleoptera: Cerambycidae). Comp Biochem Physiol (Part B) 1974; 49: 457-462.   DOI
33 Leboulle G, Rochez C, Louahed J, Ruti B, Brossard, Bollen A, Godfroid E. Isolation of Ixodes ricinus salivary gland mRNA encoding factors induced during blood feeding. Am J Trop Med Hyg 2002; 66: 225-233.   DOI
34 Rachinsky A, Guerrero FD, Scoles GA. Proteomic profiling of Rhipicephalus (Boophilus) microplus midgut responses to infection with Babesia bovis. Vet Parasitol 2008; 152: 294-313.   DOI
35 Jenkins RE, Taylor MJ, Gilvary NJ, Bianco AE. Tropomyosin implicated in host protective responses to microfilariae in onchocerciasis. Proc Natl Acad Sci USA 1998; 95: 7550-7555.   DOI