[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.3347/kjp.2015.53.1.43

Plasmodium vivax Drug Resistance Genes; Pvmdr1 and Pvcrt-o Polymorphisms in Relation to Chloroquine Sensitivity from a Malaria Endemic Area of Thailand

Rungsihirunrat, Kanchana (College of Public Health Sciences, Chulalongkorn University)
Muhamad, Poonuch (Drug Discovery and Development Center, Thammasat University)
Chaijaroenkul, Wanna (Center of Excellence in Molecular Biology and Pharmacology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University)
Kuesap, Jiraporn (Graduate Program in Biomedical Sciences, Allied Health Sciences, Thammasat University)
Na-Bangchang, Kesara (Center of Excellence in Molecular Biology and Pharmacology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University)

Publication Information

Parasites, Hosts and Diseases / v.53, no.1, 2015 , pp. 43-49 More about this Journal

Abstract

The aim of the study was to explore the possible molecular markers of chloroquine resistance in Plasmodium vivax isolates in Thailand. A total of 30 P. vivax isolates were collected from a malaria endemic area along the Thai-Myanmar border in Mae Sot district of Thailand. Dried blood spot samples were collected for analysis of Pvmdr1 and Pvcrt-o polymorphisms. Blood samples ( $100{\mu}l$ ) were collected by finger-prick for in vitro chloroquine susceptibility testing by schizont maturation inhibition assay. Based on the cut-off $IC_{50}$ of 100 nM, 19 (63.3%) isolates were classified as chloroquine resistant P. vivax isolates. Seven non-synonymous mutations and 2 synonymous were identified in Pvmdr1 gene. Y976F and F1076L mutations were detected in 7 (23.3%) and 16 isolates (53.3%), respectively. Analysis of Pvcrt-o gene revealed that all isolates were wild-type. Our results suggest that chloroquine resistance gene is now spreading in this area. Monitoring of chloroquine resistant molecular markers provide a useful tool for future control of P. vivax malaria.

Keywords

Plasmodium vivax; Pvmdr1; Pvcrt-o; drug resistance; chloroquine;

Citations & Related Records

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