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http://dx.doi.org/10.3347/kjp.2015.53.1.13

Subtype Distribution of Blastocystis in Thai-Myanmar Border, Thailand  

Popruk, Supaluk (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University)
Udonsom, Ruenruetai (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University)
Koompapong, Khuanchai (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University)
Mahittikorn, Aongart (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University)
Kusolsuk, Teera (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University)
Ruangsittichai, Jiraporn (Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University)
Palasuwan, Attakorn (Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University)
Publication Information
Parasites, Hosts and Diseases / v.53, no.1, 2015 , pp. 13-19 More about this Journal
Abstract
Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.
Keywords
Blastocystis; zoonotic risk; Thai-Myanmar border;
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1 Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 2008; 21: 639-665.   DOI
2 Yakoob J, Jafri W, Beg MA, Abbas Z, Naz S, Islam M, Khan R. Irritable bowel syndrome: is it associated with genotypes of Blastocystis hominis. Parasitol Res 2010; 106: 1033-1038.   DOI
3 Wong KH, Ng GC, Lin RT, Yoshikawa H, Taylor MB, Tan KS. Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Parasitol Res 2008; 102: 663-670.   DOI
4 Alfellani MA, Taner-Mulla D, Jacob AS, Imeede CA, Yoshikawa H, Stensvold CR, Clark CG. Genetic diversity of Blastocystis in livestock and zoo animals. Protist 2013; 164: 497-509.   DOI
5 Rene BA, Stensvold CR, Badsberg JH, Nielsen HV. Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients. Am J Trop Med Hyg 2009; 80: 588-592.
6 Souppart L, Moussa H, Cian A, Sanciu G, Poirier P, El Alaoui H, Delbac F, Boorom K, Delhaes L, Dei-Cas E, Viscogliosi E. Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt. Parasitol Res 2010; 106: 505-511.   DOI
7 Ozyurt M, Kurt O, Molbak K, Nielsen HV, Haznedaroglu T, Stensvold CR. Molecular epidemiology of Blastocystis infections in Turkey. Parasitol Int 2008; 57: 300-306.   DOI
8 Roberts T, Stark D, Harkness J, Ellis J. Subtype distribution of Blastocystis isolates identified in a Sydney population and pathogenic potential of Blastocystis. Eur J Clin Microbiol Infect Dis 2013; 32: 335-343.   DOI
9 Stensvold CR, Arendrup MC, Jespersgaard C, Molbak K, Nielsen HV. Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study. Diagn Microbiol Infect Dis 2007; 59: 303-307.   DOI
10 Leelayoova S, Taamasri P, Rangsin R, Naaglor T, Thathaisong U, Mungthin M. In-vitro cultivation: a sensitive method for detecting of Blastocystis hominis. Ann Trop Med Parasitol. 2002; 96: 803-807.   DOI
11 Termmathurapoj S, Leelayoova S, Aimpun P, Thathaisong U, Nimmanon T, Taamasri P, Mungthin M. The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens. Parasitol Res 2004; 93: 445-447.
12 Roberts T, Barratt J, Harkness J, Ellis J, Stark D. Comparison of microscopy, culture, and conventional polymerase chain reaction for detection of Blastocystis sp. in clinical stool samples. Am J Trop Med Hyg 2011; 84: 308-312.   DOI
13 Clark CG. Extensive genetic diversity in Blastocystis hominis. Mol Biochem Parasitol 1997; 87: 79-83.   DOI
14 Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23: 2947-2948.   DOI
15 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28: 2731-2739.   DOI
16 Taamasri P, Leelayoova S, Rangsin R, Naaglor T, Ketupanya A, Mungthin M. Prevalence of Blastocystis hominis carriage in Thai army personnel based in Chonburi, Thailand. Mil Med 2002; 167; 643-646.   DOI
17 Hirata T, Nakamura H, Kinjo N, Hokama A, Kinjo F, Yamane N, Fujita J. Prevalence of Blastocystis hominis and Strongyloides stercoralis infection in Okinawa, Japan. Parasitol Res 2007; 101: 1717-1719.   DOI
18 Horiki N, Maruyama M, Fujita Y, Yonekura T, Minato S, Keneda Y. Epidemiologic survey of Blastocystis hominis infection in Japan. Am J Trop Med Hyg 1997; 56: 370-374.   DOI
19 Saksirisampant W, Nuchprayoon S, Wiwanitkit V, Yenthakam S, Ampavasiri A. Intestinal parasitic infestations among children in an orphanage in Pathum Thani Province. J Med Assoc Thai 2003; 86: S263-S270.
20 Mungthin M, Suwannasaeng R, Naaglor T, Areekul W, Leelayoova S. Asymptomatic intestinal microsporidiosis in Thai orphans and child-care workers. Trans R Soc Trop Med Hyg 2001; 95: 304-306.   DOI
21 Meloni D, Sanciu G, Poirier P, El Alaoui H, Chabe M, Delhaes L, Dei-Cas E, Delbac F, Luigi Fiori P, Di Cave D, Viscogliosi E. Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy. Parasitol Res 2011; 109: 613-619.   DOI
22 Yoshikawa H, Wu Z, Kimata I, Iseki M, Karim I, Hossain AMB, Zaman V, Haque R, Takahashi Y. Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries. Parasitol Res 2004; 92: 22-29.   DOI
23 Thathaisong U, Siripattanapipong S, Mungthin M, Pipatsatitpong D, Tan-Ariya P, Naaglor T, Leelayoova S. Identification of Blastocystis subtype 1 variants in the Home for Girls, Bangkok, Thailand. Am J Trop Med Hyg 2013; 88: 352-358.   DOI
24 Yoshikawa H, Wu Z, Pandey K, Pandey BD, Sherchand JB, Yanagi T, Kanbara H. Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal. Vet Parasitol 2009; 160: 295-300.   DOI
25 Leelayoova S, Siripattanapipong S, Thathaisong U, Naaglor T, Taamasri P, Piyaraj P, Mungthin M. Drinking water: a possible source of Blastocystis spp. subtype 1 infection in schoolchildren of a rural community in central Thailand. Am J Trop Med Hyg 2008; 79: 401-406.
26 Thathaisong U, Worapong J, Mungthin M, Tan-Ariya P, Viputtigul K, Sudatis A, Noonai A, Leelayoova S. Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis. J Clin Microbiol 2003; 41: 967-975.   DOI
27 Parkar U, Traub RJ, Vitali S, Elliot A, Levecke B, Robertson I, Geurden T, Steele J, Drake B, Thompson RC. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet Parasitol 2010; 169: 8-17.   DOI
28 Abe N. Molecular and phylogenetic analysis of Blastocystis isolates from various hosts. Vet Parasitol 2004; 120: 235-242.   DOI
29 Silberman JD, Sogin ML, Leipe DD, Clark CG. Human parasite finds taxonomic home. Nature 1996; 380: 398.   DOI
30 Rivera WL. Phylogenetic analysis of Blastocystis isolates from animal and human hosts in the Philippines. Vet Parasitol 2008; 156: 178-182.   DOI
31 Arisue N, Hashimoto T, Yoshikawa H. Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates. Parasitology 2003; 126: 1-9.   DOI
32 Noel C, Peyronnet C, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Sogin ML, Capron M, Viscogliosi E, Zenner L. Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences. Mol Biochem Parasitol 2003; 126: 119-123.   DOI
33 Stensvold CR, Clark CG. Investigation of the molecular epidemiology of Blastocystis by use of a multi-locus sequence typing system for subtype 3 (personal communication).
34 Stensvold CR, Alfellani M, Clark CG. Levels of genetic diversity vary dramatically between Blastocystis subtypes. Infect Genet Evol 2012; 12: 263-273.   DOI
35 Yan MY, Su LS, Lai YR, Ye HJ, Liao H, Chen FG, Luo PX, Hou PZ, Lai FX. Phylogenetic analysis of Blastocystis hominis isolates in China (personal communication).
36 Engsbro AL, Stensvold CR. Blastocystis: to treat or not to treat...but how? Clin Infect Dis 2012; 55: 1431-1432.   DOI
37 Yoshikawa H. Phylogenetic studies of Blastocystis isolates from zoo marsupial mammals inferred from the small subunit ribosomal RNA gene sequences (personal communication).