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http://dx.doi.org/10.3347/kjp.2014.52.4.443

Molecular Detection and Seroprevalence of Babesia microti among Stock Farmers in Khutul City, Selenge Province, Mongolia  

Hong, Sung-Hee (Division of Malaria &Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Anu, Davaasuren (Laboratory of Parasitology, National Center for Communicable Diseases, Ministry of Health)
Jeong, Young-Il (Division of Malaria &Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Abmed, Davaajav (Laboratory of Parasitology, National Center for Communicable Diseases, Ministry of Health)
Cho, Shin-Hyeong (Division of Malaria &Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Lee, Won-Ja (Division of Malaria &Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Lee, Sang-Eun (Division of Malaria &Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Publication Information
Parasites, Hosts and Diseases / v.52, no.4, 2014 , pp. 443-447 More about this Journal
Abstract
Babesiosis is an emerging tick-borne disease in humans worldwide; however, little is known about the frequency of infection or prevalence of this disease in other parts of the world, excluding North America. In this study, we aimed to investigate Babesia microti infection frequency in a human population in Mongolia. One hundred blood samples were collected from stock farmers living in Khutul city of Selenge province, Mongolia. The sera and DNA from blood samples were evaluated for the presence of B. microti infection by using indirect fluorescent antibody (IFA) tests and PCR. The positive detection rates obtained using the IFA tests and PCR assays were 7% and 3%, respectively. This study is the first to detect of B. microti infections based on antibody seroprevalence or PCR assays for the presence of B. microti DNA in a Mongolian population.
Keywords
Babesia microti; IFA test; PCR; Mongolia;
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1 Rar VA, Epikhina TI, Livanova NN, Pukhovskaia NM, Vysochina NP, Ivanov LI. Detection of Babesia spp. DNA in small mammals and ixodic ticks on the territory of north Ural, west Siberia and far east of Russia. Mol Gen Microbiol Virusol 2010; 3:26-30.
2 Hao GF, Li H, Sun Y, Ge RP, Qia GQ, Li B, Tian WZ, Shi NX, Yang XY. Detection of tick and tick-borne pathogen in some ports of Inner Mongolia. Zhonghua Liu Xing Bing Xue Za Zhi 2009; 30: 365-367.
3 Zamoto A, Tsuji M, Kawabuchi T, Wei Q, Asakawa M, Ishihara C. U.S.-type Babesia microti isolated from small wild mammals in Eastern Hokkaido, Japan. J Vet Med Sci 2004; 66: 919-926.   DOI
4 Avarzed A, De Waal DT, Igarashi I, Saito A, Oyamada T, Toyoda Y, Suzuki N. Prevalence of equine piroplasmosis in Central Mongolia. Onderstepoort J Vet Res 1997; 64: 141-145.
5 Sivakumar T, Altangerel K, Battsetseg B, Battur B, Aboulaila M, Munkhjargal T, Yoshinari T, Yokoyama N, Igarashi I. Genetic detection of Babesia bigemina from Mongolian cattle using apical membrane antigen-1 gene-based PCR assay. Vet Parasitol 2012; 187: 17-22.   DOI
6 Malandrin L, L Hostis M, Chauvin A. Isolation of Babesia divergens from carrier cattle blood using in vitro culture. Vet Res 2004; 35: 131-139.   DOI
7 Krause PJ, Telford SR 3rd, Spielman A, Sikand V, Ryan R, Christianson D, Burke G, Brassard P, Pollack R, Peck J, Persing DH. Concurrent Lyme disease and babesiosis. Evidence for increased severity and duration of illness. JAMA 1996; 275: 1657-1660.   DOI
8 Vannier E, Krause PJ. Human babesiosis. N Engl J Med 2012; 366: 2397-2407.   DOI
9 Welc-Faleciak R, Bajer A, Bednarska M, Paziewska A, Sinski E. Long term monitoring of Babesia microti infection in BALB/c mice using nested PCR. Ann Agric Environ Med 2007; 14: 287-290.
10 Sun Y, Liu G, Yang L, Xu R, Cao W. Babesia microti-like rodent parasites isolated from Ixodes persulcatus (Acari: Ixodidae) in Heilongjiang Province, China. Vet Parasitol 2008; 156: 333-339.   DOI
11 Homer MJ, Aguilar-Delfin I, Telford SR 3rd, Krause PJ, Persing DH. Babesiosis. Clin Microbiol Rev 2000; 13: 451-469.   DOI   ScienceOn
12 Herwaldt BL, Caccio S, Gherlinzoni F, Aspock H, Slemenda SB, Piccaluga P, Martinelli G, Edelhofer R, Hollenstein U, Poletti G, Pampiglione S, Loschenberger K, Tura S, Pieniazek NJ. Molecular characterization of a non-Babesia divergens organism causing zoonotic babesiosis in Europe. Emerg Infect Dis 2003; 9: 942-948.   DOI
13 Spielman A. Human babesiosis on Nantucket Island: transmission by nymphal Ixodes ticks. Am J Trop Med Hyg 1976; 25: 784-787.
14 Vannier E, Gewurz BE, Krause PJ. Human babesiosis. Infect Dis Clin North Am 2008; 22: 469-488.   DOI
15 Hunfeld KP, Brade V. Zoonotic Babesia: possibly emerging pathogens to be considered for tick-infested humans in Central Europe. Int J Med Microbiol 2004; 293 (supp. 37): 93-103.
16 Persing DH, Herwaldt BL, Glaser C, Lane RS, Thomford JW, Mathiesen D, Krause PJ, Phillip DF, Conrad PA. Infection with a Babesia- like organism in northern California. N Engl J Med 1995; 332: 298-303.   DOI
17 Hunfeld KP, Lambert A, Kampen H, Albert S, Epe C, Brade V, Tenter AM. Seroprevalence of Babesia infections in humans exposed to ticks in midwestern Germany. J Clin Microbiol 2002; 40: 2431-2436.   DOI
18 Krause PJ, Telford SR 3rd, Ryan R, Conrad PA, Wilson M, Thomford JW, Spielman A. Diagnosis of babesiosis: evaluation of a serologic test for the detection of Babesia microti antibody. J Infect Dis 1994; 169: 923-926.   DOI   ScienceOn
19 Poyraz O, Gunes T. Seroprevalance of Babesia microti in humans living in rural areas of the Sinop region. Turkiye Parazitol Derg 2010; 34: 81-85.
20 Conrad PA, Jemtrup AM, Carreno RA, Thomford J, Wainwright K, Eberhard M, Quick R, Telford SR3rd, Herwaldt BL. Description of Babesia duncani n.sp. (Apicomplexa: Babesiidae) from humans and its differentiation from other piroplasms. Int J Parasitol 2006; 36: 779-789.   DOI
21 Leiby DA. Babesiosis and blood transfusion: flying under the radar. Vox Sang 2006; 90: 157-165.   DOI
22 Popovsky MA. Transfusion-transmitted babesiosis. Transfusion 1991; 31: 296-298.   DOI
23 Shih CM, Liu LP, Chung WC, Ong SJ, Wang CC. Human babesiosis in Taiwan: asymptomatic infection with a Babesia microtilike organism in a Taiwanese woman. J Clin Microbiol 1997; 35: 450-454.
24 Saito-Ito A, Tsuji M, Wei Q, He S, Matsui T, Kohsaki M, Arai S, Kamiyama T, Hioki K, Ishihara C. Transfusion-acquired, autochthonous human babesiosis in Japan: isolation of Babesia microtilike parasites with hu-RBC-SCID mice. J Clin Microbiol 2000; 38: 4511-4516.
25 Kim JY, Cho SH, Joo HN, Tsuji M, Cho SR, Park IJ, Chung GT, Ju JW, Cheun HI, Lee HW, Lee YH, Kim TS. First case of human babesiosis in Korea: detection and characterization of a novel type of Babesia sp. (KO1) similar to ovine Babesia. J Clin Microbiol 2007; 45: 2084-2087.   DOI   ScienceOn
26 Battsetseg B, Xuan X, Ikadai H, Bautista JL, Byambaa B, Boldbaatar D, Battur B, Battsetseg G, Batsukh Z, Igarashi I, Nagasawa H, Mikami T, Fujisaki K. Detection of Babesia caballi and Babesia equi in Dermacentor nuttalli adult ticks. Int J Parasitol 2001; 31: 384-386.   DOI
27 Battsetseg B, Lucero S, Xuan X, Claveria F, Byambaa B, Battur B, Boldbaatar D, Batsukh Z, Khaliunaa T, Battsetseg G, Igarashi I, Nagasawa H, Fujisaki K. Detection of equine Babesia spp. gene fragments in Dermacentor nuttalli Olenev 1929 infesting mongolian horses, and their amplification in egg and larval progenies. J Vet Med Sci 2002; 64: 727-730.   DOI
28 Holger C, Scholz. Margos G, Derschum H, Speck S, Tserennorov D, Erdenebat N, Undraa M, Enkhtuja B, Battsetseg J, Otgonchimeg C, Otgonsuren G , Nymadulam B, Romer A, Thomas A, Essbauer S, Wolfel R, Kiefer D, Zoller L, Otgonbaatar D, Fingerle V. High prevalence of genetically diverse Borrelia bavariensis-like strains in Ixodes persulcatus from Selenge Aimag, Mongolia. Ticks Tick Borne Dis 2013; 4: 89-92.   DOI