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http://dx.doi.org/10.3347/kjp.2014.52.4.377

Nested-PCR and a New ELISA-Based NovaLisa Test Kit for Malaria Diagnosis in an Endemic Area of Thailand  

Thongdee, Pimwan (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University)
Chaijaroenkul, Wanna (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University)
Kuesap, Jiraporn (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University)
Na-Bangchang, Kesara (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University)
Publication Information
Parasites, Hosts and Diseases / v.52, no.4, 2014 , pp. 377-381 More about this Journal
Abstract
Microscopy is considered as the gold standard for malaria diagnosis although its wide application is limited by the requirement of highly experienced microscopists. PCR and serological tests provide efficient diagnostic performance and have been applied for malaria diagnosis and research. The aim of this study was to investigate the diagnostic performance of nested PCR and a recently developed an ELISA-based new rapid diagnosis test (RDT), NovaLisa test kit, for diagnosis of malaria infection, using microscopic method as the gold standard. The performance of nested-PCR as a malaria diagnostic tool is excellent with respect to its high accuracy, sensitivity, specificity, and ability to discriminate Plasmodium species. The sensitivity and specificity of nested-PCR compared with the microscopic method for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax mixed infection were 71.4 vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa test kit compared with the microscopic method for detection of Plasmodium genus were 89.0 vs 91.6%, respectively. NovaLisa test kit provided comparable diagnostic performance. Its relatively low cost, simplicity, and rapidity enables large scale field application.
Keywords
Plasmodium falciparum; Plasmodium vivax; nested-PCR; ELISA-based NovaLisa test kit;
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Times Cited By KSCI : 2  (Citation Analysis)
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