Browse > Article
http://dx.doi.org/10.3347/kjp.2013.51.6.747

Molecular Detection of Ancylostoma duodenale, Ancylostoma ceylanicum, and Necator americanus in Humans in Northeastern and Southern Thailand  

Phosuk, Issarapong (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Intapan, Pewpan M. (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Thanchomnang, Tongjit (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Sanpool, Oranuch (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Janwan, Penchom (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Laummaunwai, Porntip (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Aamnart, Witthaya (Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University)
Morakote, Nimit (Department of Parasitology, Faculty of Medicine Chiang Mai University)
Maleewong, Wanchai (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Publication Information
Parasites, Hosts and Diseases / v.51, no.6, 2013 , pp. 747-749 More about this Journal
Abstract
The 2 principal species of hookworms infecting humans are Necator americanus and Ancylostoma duodenale. Case studies on zoonotic hookworm infections with Ancylostoma ceylanicum and/or Ancylostoma caninum are known mainly from Asian countries. Of these 2 zoonotic species, only A. ceylanicum can develop to adulthood in humans. In the present study, we report a molecular-based survey of human hookworm infections present in southern and northeastern Thailand. Thirty larval hookworm samples were obtained from fecal agar plate cultures of 10 patients in northeastren Thailand and 20 in southern Thailand. Partial ITS1, 5.8S, and ITS2 regions of the ribosomal DNA genes were amplified using PCR. The amplicons were sequenced, aligned, and compared with other hookworm sequences in GenBank database. The results showed that, in Thailand, N. americanus is more prevalent than Ancylostoma spp. and is found in both study areas. Sporadic cases of A. ceylanicum and A. duodenale infection were seen in northeastern Thailand.
Keywords
Ancylostoma duodenale; Ancylostoma ceylanicum; Necator americanus; human; molecular detection;
Citations & Related Records
연도 인용수 순위
  • Reference
1 de Silva NR, Brooker S, Hotez PJ, Montresor A, Engels D, Savioli L. Soil-transmitted helminth infections: updating the global picture. Trends Parasitol 2003; 19: 547-551.   DOI   ScienceOn
2 Mahdy MA, Lim YA, Ngui R, Siti Fatimah MR, Choy SH, Yap NJ, Al-Mekhlafi HM, Ibrahim J, Surin J. Prevalence and zoonotic potential of canine hookworms in Malaysia. Parasit Vectors 2012; 5: 88.   DOI
3 Traub RJ, Inpankaew T, Sutthikornchai C, Sukthana Y, Thompson RC. PCR-based coprodiagnostic tools reveal dogs as reservoirs of zoonotic ancylostomiasis caused by Ancylostoma ceylanicum in temple communities in Bangkok. Vet Parasitol 2008; 155: 67-73.   DOI   ScienceOn
4 Jex AR, Lim YA, Bethony JM, Hotez PJ, Young ND, Gasser RB. Soil-transmitted helminths of humans in Southeast Asia--towards integrated control. Adv Parasitol 2011; 74: 231-265.   DOI   ScienceOn
5 Jiraanankul V, Aphijirawat W, Mungthin M, Khositnithikul R, Rangsin R, Traub RJ, Piyaraj P, Naaglor T, Taamasri P, Leelayoova S. Incidence and risk factors of hookworm infection in a rural community of central Thailand. Am J Trop Med Hyg 2011; 84: 594-598.   DOI   ScienceOn
6 Sato M, Sanguankiat S, Yoonuan T, Pongvongsa T, Keomoungkhoun M, Phimmayoi I, Boupa B, Moji K, Waikagul J. Copromolecular identification of infections with hookworm eggs in rural Lao PDR. Trans R Soc Trop Med Hyg 2010; 104: 617-622.   DOI   ScienceOn
7 Schneider B, Jariwala AR, Periago MV, Gazzinelli MF, Bose SN, Hotez PJ, Diemert DJ, Bethony JM. A history of hookworm vaccine development. Hum Vaccin 2011; 7: 1234-1244.   DOI
8 Crompton DW. The public health importance of hookworm disease. Parasitology 2000; 121 (suppl): 39-50.   DOI   ScienceOn
9 Chan MS, Medley GF, Jamison D, Bundy DA. The evaluation of potential global morbidity attributable to intestinal nematode infections. Parasitology 1994; 109: 373-387.   DOI
10 Arakaki T, Iwanaga M, Kinjo F, Saito A, Asato R, Ikeshiro T. Efficacy of agar plate culture in detection of Strongyloides stercoralis infection. J Parasitol 1990; 76: 425-428.   DOI   ScienceOn
11 Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22: 4673-4680.   DOI   ScienceOn
12 Ngui R, Ching LS, Kai TT, Roslan MA, Lim YA. Molecular identification of human hookworm infections in economically disadvantaged communities in Peninsular Malaysia. Am J Trop Med Hyg 2012; 86: 837-842.   DOI
13 Beaver PC, Jung RC, Cupp EW. Clinical Parasitology. 9th ed. Philadelphia, USA. Lea & Febiger. 1984, p 269-287.
14 Hoagland KE, Schad GA. Necator americanus and Ancylostoma duodenale: life history parameters and epidemiological implications of two sympatric hookworms of humans. Exp Parasitol 1978; 44: 36-49.   DOI   ScienceOn