Browse > Article
http://dx.doi.org/10.3347/kjp.2013.51.6.703

Distribution and Abundance of Opisthorchis viverrini Metacercariae in Cyprinid Fish in Northeastern Thailand  

Pinlaor, Somchai (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Onsurathum, Sudarat (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Boonmars, Thidarut (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Pinlaor, Porntip (Department of Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University)
Hongsrichan, Nuttanan (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Chaidee, Apisit (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Haonon, Ornuma (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Limviroj, Wutipong (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Tesana, Smarn (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Kaekews, Sasithorn (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Sithithaworn, Paiboon (Department of Parasitology, Faculty of Medicine, Khon Kaen University)
Publication Information
Parasites, Hosts and Diseases / v.51, no.6, 2013 , pp. 703-710 More about this Journal
Abstract
To increase public health awareness for prevention of opisthorchiasis caused by eating raw freshwater fish, the distribution and abundance of Opisthorchis viverrini metacercariae (OV MC) was investigated in freshwater fish obtained from 20 provinces in northeastern Thailand between April 2011 and February 2012. A cross-sectional survey was conducted on 12,890 fish consisting of 13 species randomly caught from 26 rivers, 10 dams, and 38 ponds/lakes. Fish, were collected in each of the rainy and winter seasons from each province. Fish were identified, counted, weighed, and digested using pepsin-HCl. Samples were examined for OV MC by a sedimentation method, and metacercariae were identified under a stereomicroscope. OV MC were found in 6 species of fish; i.e., Cyclocheilichthys armatus, Puntius orphoides, Hampala dispar, Henicorhynchus siamensis, Osteochilus hasselti, and Puntioplites proctozysron from localities in 13 provinces. Among the sites where OV MC-infected fish were found, 70.0% were dams, 23.7% were ponds/lakes, and 7.7% were rivers. The mean intensity of OV MC ranged from 0.01 to 6.5 cysts per fish (or 1.3-287.5 cysts per kg of fish). A high mean intensity of OV MC per fish (>3 cysts) was found in 5 provinces: Amnat Charoen (6.5 cysts), Nakhon Phanom (4.3), Mukdahan (4.1), Khon Kaen, (3.5) and Si Sa Ket (3.4). In conclusion, OV MC are prevalent in natural cyprinid fish, with the infection rate varying according to fish species and habitats.
Keywords
Opisthorchis viverrini; metacercaria; foodborne trematode; cyprinid fish; distribution; intensity;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Kiatsopit N, Sithithaworn P, Boonmars T, Tesana S, Chanawong A, Saijuntha W, Petney TN, Andrews RH. Genetic markers for studies on the systematics and population genetics of snails, Bithynia spp., the first intermediate hosts of Opisthorchis viverrini in Thailand. Acta Trop 2011; 118: 136-141.   DOI   ScienceOn
2 Sithithaworn P, Andrews RH, Nguyen VD, Wongsaroj T, Sinuon M, Odermatt P, Nawa Y, Liang S, Brindley PJ, Sripa B. The current status of opisthorchiasis and clonorchiasis in the Mekong Basin. Parasitol Int 2012; 61: 10-16.   DOI   ScienceOn
3 Aunpromma S, Tangkawattana P, Papirom P, Kanjampa P, Tesana S, Sripa B, Tangkawattana S. High prevalence of Opisthorchis viverrini infection in reservoir hosts in four districts of Khon Kaen Province, an opisthorchiasis endemic area of Thailand. Parasitol Int 2012; 61: 60-64.   DOI   ScienceOn
4 Kaewpitoon SJ, Rujirakul R, Kaewpitoon N. Prevalence of Opisthorchis viverrini infection in Nakhon Ratchasima province, Northeast Thailand. Asian Pac J Cancer Prev 2012; 13: 5245-5249.   DOI   ScienceOn
5 Saengsawang P, Promthet S, Bradshaw P. Prevalence of OV infection in Yasothon Province, Northeast Thailand. Asian Pac J Cancer Prev 2012; 13: 3399-3402.   DOI   ScienceOn
6 Forrer A, Sayasone S, Vounatsou P, Vonghachack Y, Bouakhasith D, Vogt S, Glaser R, Utzinger J, Akkhavong K, Odermatt P. Spatial distribution of, and risk factors for, Opisthorchis viverrini infection in southern Lao PDR. PLoS Negl Trop Dis 2012; 6: e1481.   DOI
7 Srisawangwong T, Sithithaworn P, Tesana S. Metacercariae isolated from cyprinoid fishes in Khon Kaen District by digestion technic. Southeast Asian J Trop Med Public Health 1997; 28(suppl 1): 224-226.
8 Sukontason K, Piangjai S, Muangyimpong Y, Methanitikorn R, Chaithong U. Prevalence of trematode metacercariae in cyprinoid fish of Ban Pao district, Chiang Mai Province, northern Thailand. Southeast Asian J Trop Med Public Health 1999; 30: 365-370.
9 Vichasri S, Viyanant V, Upatham ES. Opisthorchis viverrini : intensity and rates of infection in cyprinoid fish from an endemic focus in Northeast Thailand. Southeast Asian J Trop Med Public Health 1982; 13: 138-141.
10 Sithithaworn P, Ziegler AD, Grundy-Warr C, Andrews RH, Petney TN. Changes to the life cycle of liver flukes: dams, roads, and ponds. Lancet Infect Dis 2012; 12: 588.   DOI   ScienceOn
11 Kaewpitoon N, Kaewpitoon SJ, Ueng-arporn N, Rujirakul R, Churproong S, Matrakool L, Auiwatanagul S, Sripa B. Carcinogenic human liver fluke: current status of Opisthorchis viverrini metacercariae in Nakhon Ratchasima, Thailand. Asian Pac J Cancer Prev 2012; 13: 1235-1240.   DOI   ScienceOn
12 Nithiuthai S, Suwansaksri J, Wiwanitkit V, Chaengphukeaw P. A survey of metacercariae in cyprinoid fish in Nakhon Ratchasima, northeast Thailand. Southeast Asian J Trop Med Public Health 2002; 33(suppl 3): 103-105.
13 Phan VT, Ersboll AK, Nguyen TT, Nguyen KV, Nguyen HT, Murrell D, Dalsgaard A. Freshwater aquaculture nurseries and infection of fish with zoonotic trematodes, Vietnam. Emerg Infect Dis 2010; 16: 1905-1909.   DOI
14 Stumbo AD, James CT, Goater CP, Wisenden BD, Cotter S. Shoaling as an antiparasite defence in minnows (Pimephales promelas) exposed to trematode cercariae. J Anim Ecol 2012; 81: 1319-1326.   DOI   ScienceOn
15 Wang YC. Examining landscape determinants of Opisthorchis viverrini transmission. EcoHealth 2012; 9: 328-341.   DOI   ScienceOn
16 Bush AO, Lafferty KD, Lotz JM, Shostak AW. Parasitology meets ecology on its own terms: Margolis et al. revisited. J Parasitol 1997; 83: 575-583.   DOI   ScienceOn
17 Touch S, Komalamisra C, Radomyos P, Waikagul J. Discovery of Opisthorchis viverrini metacercariae in freshwater fish in southern Cambodia. Acta Trop 2009; 111: 108-113.   DOI   ScienceOn
18 Sohn WM, Yong TS, Eom KS, Pyo KH, Lee MY, Lim H, Choe S, Jeong HG, Sinuon M, Socheat D, Chai JY. Prevalence of Opisthorchis viverrini infection in humans and fish in Kratie Province, Cambodia. Acta Trop 2012; 124: 215-220.   DOI   ScienceOn
19 Rim HJ, Sohn WM, Yong TS, Eom KS, Chai JY, Min DY, Lee SH, Hoang EH, Phommasack B, Insisiengmay S. Fishborne trematode metacercariae in Luang Prabang, Khammouane, and Saravane Province, Lao PDR. Korean J Parasitol 2013; 51: 107-114.   DOI   ScienceOn
20 Waikagul J. Opisthorchis viverrini metacercaria in Thai freshwater fish. Southeast Asian J Trop Med Public Health 1998; 29: 324-326.
21 Rim HJ, Sohn WM, Yong TS, Eom KS, Chai JY, Min DY, Lee SH, Hoang EH, Phommasack B, Insisengmay S. Fishborne trematode metacercariae detected in freshwater fish from Vientiane Municipality and Savannakhet Province, Lao PDR. Korean J Parasitol 2008; 46: 253-260.   DOI
22 Dudgeon D. Asian river fishes in the Anthropocene: threats and conservation challenges in an era of rapid environmental change. J Fish Biol 2011; 79: 1487-1524.   DOI   ScienceOn
23 Sithithaworn P, Pipitgool V, Srisawangwong T, Elkins DB, Haswell- Elkins MR. Seasonal variation of Opisthorchis viverrini infection in cyprinoid fish in north-east Thailand: implications for parasite control and food safety. Bull World Health Organ 1997; 75: 125-131.
24 Chuboon S, Wongsawad C, Ruamsuk A, Nithikathkul C. Survival of Haplorchis taichui metacercariae in Lab-Pla, Thai traditional food preparation. Southeast Asian J Trop Med Public Health 2005; 36(suppl 4): 110-111.
25 Prasongwatana J, Laummaunwai P, Boonmars T, Pinlaor S. Viable metacercariae of Opisthorchis viverrini in northeastern Thai cyprinid fish dishes-as part of a rational program for control of O. viverrini-associated cholangiocarcinoma. Parasitol Res 2013; 112: 1323-1327.   DOI   ScienceOn
26 Pitaksakulrat O, Sithithaworn P, Laoprom N, Laha T, Petney TN, Andrews RH. A cross-sectional study on the potential transmission of the carcinogenic liver fluke Opisthorchis viverrini and other fishborne zoonotic trematodes by aquaculture fish. Foodborne Pathog Dis 2013; 10: 35-41.   DOI   ScienceOn
27 Vajrasthira S, Harinasuta C, Komiya Y. The morphology of the metacercaria of Opisthorchis viverrini, with special reference to the excretory system. Ann Trop Med Parasitol 1961; 55: 413-418.
28 IARC. A review of human carcinogens: Opisthorchis viverrini and Clonorchis sinensis. IARC Monogr Eval Carcinog Risks Hum 2012; 100B: 341-370.
29 Sripa B, Pairojkul C. Cholangiocarcinoma: lessons from Thailand. Curr Opin Gastroenterol 2008; 24: 349-356.   DOI   ScienceOn
30 Grundy-Warr C, Andrews RH, Sithithaworn P, Petney TN, Sripa B, Laithavewat L, Ziegler AD. Raw attitudes, wetland cultures, lifecycles: socio-cultural dynamics relating to Opisthorchis viverrini in the Mekong Basin. Parasitol Int 2012; 61: 65-70.   DOI   ScienceOn
31 Wang YC, Feng CC, Sithithaworn P, Feng Y, Petney TN. How do snails meet fish? Landscape perspective needed to study parasite prevalence. EcoHealth 2011; 8: 258-260.   DOI   ScienceOn