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http://dx.doi.org/10.3347/kjp.2013.51.1.85

Kinetics of IL-23 and IL-12 Secretion in Response to Toxoplasma gondii Antigens from THP-1 Monocytic Cells  

Quan, Juan-Hua (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine)
Zhou, Wei (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine)
Cha, Guang-Ho (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine)
Choi, In-Wook (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine)
Shin, Dae-Whan (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine)
Lee, Young-Ha (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.51, no.1, 2013 , pp. 85-92 More about this Journal
Abstract
IL-23 and IL-12 are structurally similar and critical for the generation of efficient cellular immune responses. Toxoplasma gondii induces a strong cell-mediated immune response. However, little is known about IL-23 secretion profiles in T. gondii-infected immune cells in connection with IL-12. We compared the patterns of IL-23 and IL-12 production by THP-1 human monocytic cells in response to stimulation with live or heat-killed T. gondii tachyzoites, or with equivalent quantities of either T. gondii excretory/secretory proteins (ESP) or soluble tachyzoite antigen (STAg). IL-23 and IL-12 were significantly increased from 6 hr after stimulation with T. gondii antigens, and their secretions were increased with parasite dose-dependent manner. IL-23 concentrations were significantly higher than those of IL-12 at the same multiplicity of infection. IL-23 secretion induced by live parasites was significantly higher than that by heat-killed parasites, ESP, or STAg, whereas IL-12 secretion by live parasite was similar to those of ESP or STAg. However, the lowest levels of both cytokines were at stimulation with heat-killed parasites. These data indicate that IL-23 secretion patterns by stimulation with various kinds of T. gondii antigens at THP-1 monocytic cells are similar to those of IL-12, even though the levels of IL-23 induction were significantly higher than those of IL-12. The detailed kinetics induced by each T. gondii antigen were different from each other.
Keywords
Toxoplasma gondii; IL-23; IL-12; THP-1 monocytic cell;
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