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http://dx.doi.org/10.3347/kjp.2012.50.4.371

Evaluation of Anti-Toxoplasma IgG, IgM, and IgA in Mothers with Spontaneous Abortion in Zanjan, Northwest Iran  

Amin, Abbas (Department of Medical Parasitology and Mycology, Medical School, Zanjan University of Medical Sciences and Health Services)
Mazloomzadeh, S. (Department of Epidemiology, Ayatollah Musavi Teaching and Treatment Centre, Zanjan University of Medical Sciences and Health Services)
Haniloo, A. (Department of Medical Parasitology and Mycology, Medical School, Zanjan University of Medical Sciences and Health Services)
Mohammadian, F. (Department of Obstetrics and Gynecology, Ayatollah Musavi Teaching and Treatment Centre, Zanjan University of Medical Sciences and Health Services)
Fazaeli, Asghar (Department of Medical Parasitology and Mycology, Medical School, Zanjan University of Medical Sciences and Health Services)
Publication Information
Parasites, Hosts and Diseases / v.50, no.4, 2012 , pp. 371-374 More about this Journal
Abstract
Toxoplasma gondii is one of the major agents of infectious abortions and due to its worldwide distribution can threat healthy pregnant women who had no previous exposure to this parasite. The present study was designed to investigate the contribution of T. gondii to spontaneous abortions in Zanjan, Northwest of Iran, using ELISA method. Blood Samples were collected from 264 mothers referred to the provincial hospitals of Zanjan due to spontaneous abortion. The sera were isolated and subjected to evaluate the anti-Toxoplasma IgG, IgM and IgA antibodies. The results showed IgG positive ($IgG^+$) in 99 cases (37.5%). A total of 68 women (25.8%) showed seroconversion with IgM or IgA or both IgM and IgA. They included: $IgM^+$ in 21 (8.0%), $IgA^+$ in 23 (8.7%) and both $IgM^+$ and $IgA^+$ in 24 (9.1%) subjects. In 23 cases, positive titers of IgM and IgG were accompanied. In general, the analysis of anti-Toxoplasma antibody patterns, showed that about 17% of the spontaneous abortions were associated with serological patterns of acute infection. According to these findings, a considerable proportion of spontaneous abortions can be attributed to T. gondii in the study area.
Keywords
Toxoplasma gondii; abortion; serology; Iran;
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  • Reference
1 Lolis D, Tzigounis V, Michalas S, Koumentakou E, Kaskarelis D. Toxoplasma antibodies and spontaneous abortion. Int J Gynaecol Obstet 1978; 15: 299-301.   DOI
2 Villena I, Ancelle T, Delmas C, Garcia P, Brezin AP, Thulliez P, Wallon M, King L, Goulet V. Congenital toxoplasmosis in France in 2007: First results from a national surveillance system. Euro Surveill 2010; 15(25): pII=19600.
3 Shoaei H. Study of the frequency of Toxoplasma infection in pregnant women referred to Imam Hossein Hospital in Zanjan using ELISA method. Dissertation to Tehran University of Medical Sciences, Tehran, Iran. 2002.
4 Haj-Soleymani F. Determination of anti-toxoplasma IgM, IgG antibodies in pregnant women referred to the medical centers of Zanjan, Iran. Dissertation to Zanjan University of Medical Sciences, Zanjan, Iran. 2008.
5 Singh J, Graniello C, Ni Y, Payne L, Sa Q, Hester J, Shelton BJ, Suzuki Y. Toxoplasma IgG and IgA, but not IgM. antibody titers increase in sera of immunocompetent mice in association with proliferation of tachyzoites in the brain during the chronic stage of infection. Microbes Infect 2010; 12: 1252-1257.   DOI   ScienceOn
6 Zargar AH, Masoodi SR, Laway BA, Sofi BA, Wani AI. Seroprevalence of toxoplasmosis in women with repeated abortions in Kashmir. J Epidemiol Community Health 1998; 52: 135-136.   DOI   ScienceOn
7 Pignanelli S. Laboratory diagnosis of Toxoplasma gondii infection with direct and indirect diagnostic techniques. Indian J Pathol Microbiol 2011; 54: 786-789.
8 Elbez-Rubinstein A, Ajzenberg A, Darde ML, Cohen R, Dumetre A,Year H, Gondon E, Janaud JC, Thulliez P. Congenital toxoplasmosis and reinfection during pregnancy: case report, strain characterization, experimental model of reinfection, and review. J Infect Dis 2009; 199: 280-285.   DOI   ScienceOn
9 Ajzenberg D, Cogne N, Paris L, Bessieres MH, Thulliez P, Filisetti D, Pelloux H, Marty P, Dardé ML. Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings. J Infect Dis 2002; 186: 684-689.   DOI   ScienceOn
10 Ajzenberg D, Year H, Marty P, Paris L, Dalle F, Menotti J, Aubert D, Franck J, Bessières MH, Quinio D, Pelloux H, Delhaes L, Desbois N, Thulliez P, Robert-Gangneux F, Kauffmann-Lacroix C, Pujol S, Rabodonirina M, Bougnoux ME, Cuisenier B, Duhamel C, Duong TH, Filisetti D, Flori P, Gay-Andrieu F, Pratlong F, Nevez G, Totet A, Carme B, Bonnabau H, Darde ML, Villena I. Genotype of 88 Toxoplasma gondii isolates associated with toxoplasmosis in immunocompromised patients and correlation with clinical findings. J Infect Dis 2009; 199: 1155-1167.   DOI   ScienceOn
11 Howe DK, Honore S, Derouin F, Sibley LD. Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis. J Clin Microbiol 1997; 35: 1411-1414.
12 Zia-Ali N, Fazaeli A, Khoramizadeh M, Ajzenberg D, Darde M, Keshavarz-Valian H. Isolation and molecular characterization of Toxoplasma gondii strains from different hosts in Iran. Parasitol Res 2007; 101: 111-115.   DOI   ScienceOn
13 Montoya JG, Kovacs JA, Remington JS. Toxoplasma gondii. In Mandell GL, Benett JE, Dolin R, Mandell D eds, Principles and Practice of Infectious Disease. Philadelphia, USA. Elsevier Churchill Livingstone. 2005, p 3170-3198.
14 Dubey JP, Hill D. Toxoplasma gondii: Transmission and Prevention. Clin Microbiol Rev 2002; 8: 634-640.
15 Hall S, Ryan M, Buxton D. The epidemiology of toxoplasma infection. In Joynson DHM, Wreghitt TG eds, Toxoplasmosis: a Comprehensive Clinical Guide. Cambridge, UK. Cambridge University Press. 2001, p 58-65.
16 Joynson DHM, Guy EC. Laboratory diagnosis of toxoplasma infection. In Joynson DHM, Wreghitt TG eds, Toxoplasmosis: a Comprehensive Clinical Guide. Cambridge, UK. Cambridge University Press. 2001, p 296-318.