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http://dx.doi.org/10.3347/kjp.2012.50.4.301

Experimental Murine Fascioliasis Derives Early Immune Suppression with Increased Levels of TGF-${\beta}$ and IL-4  

Chung, Joon-Yong (Tissue Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, NIH)
Bae, Young-An (Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Centers for Molecular Medicine, Samsung Biomedical Research Institute)
Yun, Doo-Hee (Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Centers for Molecular Medicine, Samsung Biomedical Research Institute)
Yang, Hyun-Jong (Department of Parasitology, Ewha Womans University School of Medicine)
Kong, Yoon (Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Centers for Molecular Medicine, Samsung Biomedical Research Institute)
Publication Information
Parasites, Hosts and Diseases / v.50, no.4, 2012 , pp. 301-308 More about this Journal
Abstract
In fascioliasis, T-helper 2 (Th2) responses predominate, while little is known regarding early immune phenomenon. We herein analyzed early immunophenotype changes of BALB/c, C57BL/6, and C3H/He mice experimentally infected with 5 Fasciola hepatica metacercariae. A remarkable expansion of $CD19^+$ B cells was observed as early as week 1 post-infection while $CD4^+/CD8^+$ T cells were down-regulated. Accumulation of $Mac1^+$ cells with time after infection correlated well with splenomegaly of all mice strains tested. The expression of tumor necrosis factor (TNF)-${\alpha}$ mRNA in splenocytes significantly decreased while that of IL-4 up-regulated. IL-$1{\beta}$ expression was down-modulated in BALB/c and C57BL/6 mice, but not in C3H/He. Serum levels of transforming growth factor (TGF)-${\beta}$ were considerably elevated in all mice during 3 weeks of infection period. These collective results suggest that experimental murine fascioliasis might derive immune suppression with elevated levels of TGF-${\beta}$ and IL-4 during the early stages of infection.
Keywords
Fasciola hepatica; murine fascioliasis; Mac $1^+$ cell; T-lymphocyte; IL-4; TGF-${\beta}$;
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