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http://dx.doi.org/10.3347/kjp.2012.50.3.229

Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR  

Lee, Sang-Eun (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Hong, Sung-Hee (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Lee, Seong-Ho (R & D Center, Lumieye Genetics Co. Ltd.)
Jeong, Young-Il (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Lim, Su-Jin (The Retina Center, Nune Eye Hospital)
Kwon, Oh-Woong (The Retina Center, Nune Eye Hospital)
Kim, Sun-Hyun (The Retina Center, Nune Eye Hospital)
You, Young-Sung (The Retina Center, Nune Eye Hospital)
Cho, Shin-Hyeong (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Lee, Won-Ja (Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention)
Publication Information
Parasites, Hosts and Diseases / v.50, no.3, 2012 , pp. 229-231 More about this Journal
Abstract
Toxoplasma gondii is a zoonotic parasite resulting in human infections and one of the infectious pathogens leading to uveitis and retinochoroiditis. The present study was performed to assess T. gondii infection in 20 ocular patients with chronic irregular recurrent uveitis (20 aqueous humor and 20 peripheral blood samples) using PCR. All samples were analyzed by nested PCR targeting a specific B1 gene of T. gondii. The PCR-positive rate was 25% (5/20), including 5% (1) in blood samples, 25% (5) in aqueous humor samples, and 5% (1) in both sample types. A molecular screening test for T. gondii infection in ocular patients with common clinical findings of an unclear retinal margin and an inflammatory membrane over the retina, as seen by fundus examination, may be helpful for early diagnosis and treatment.
Keywords
Toxoplasma gondii; toxoplasmosis; uveitis; nested PCR; B1 gene;
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Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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