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http://dx.doi.org/10.3347/kjp.2012.50.2.99

IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans  

Rahbari, Amir Hossien (Tehran University of Medical Sciences)
Keshavarz, Hossien (Tehran University of Medical Sciences)
Shojaee, Saeedeh (Tehran University of Medical Sciences)
Mohebali, Mehdi (Tehran University of Medical Sciences)
Rezaeian, Mostafa (Tehran University of Medical Sciences)
Publication Information
Parasites, Hosts and Diseases / v.50, no.2, 2012 , pp. 99-102 More about this Journal
Abstract
Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels ($AI{\leq}50$), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.
Keywords
Toxoplasma gondii; IgG avidity test; ELISA; IgM; indirect immunofluorescent antibody test;
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1 Leite M, Siciliano S, Rocha LS, Justa MT, César KR, Granato CF. Correlation between specific IgM levels and percentage IgG-class antibody avidity to Toxoplasma gondii. Rev Inst Med Trop Sao Paulo 2008; 50: 237-242.
2 Iqbal J, Khalid N. Detection of acute Toxoplasma gondii infection in early pregnancy by IgG avidity and PCR analysis. J Med Microbiol 2007; 56(Pt 11): 1495-1499.
3 Candolfi E, Pastor R, Huber R, Filisetti D, Villard O. IgG avidity assay firms up the diagnosis of acute toxoplasmosis on the first serum sample in immunocompetent pregnant women. Diagn Microbiol Infect Dis 2007; 58: 83-88.
4 Crucerescu E, Lovin DR. Study on specific IgG avidity as a tool for recent primary Toxoplasma gondii infection diagnosis. J Prev Med 2002; 10: 56-62.
5 Roberts A, Hedman K, Luyasu V, Zufferey J, Bessières MH, Blatz RM, Candolfi E, Decoster A, Enders G, Gross U, Guy E, Hayde M, Ho-Yen D, Johnson J, Lécolier B, Naessens A, Pelloux H, Thulliez P, Petersen E. Multicenter evaluation of strategies for serodiagnosis of primary infection with Toxoplasma gondii. Eur J Clin Microbiol Infect Dis 2001; 20: 467-474.
6 Hedman K, Lappalainen M, Sönderlund M, Hedman L. Avidity of IgG in serodiagnosis of infectious diseases. Rev Med Microbiol 1993; 4: 123-129.
7 Marcolino PT, Silva DAO, Leser PG, Camargo ME, Mineo JR. Molecular markers in acute and chronic phases of human toxoplasmosis: determination of immunoglobulin G avidity by Western blotting. Clin Diagn Lab Immunol 2000; 7: 384-389.
8 Hedman K, Lappalainen M, Seppaia I, Makela O. Recent primary toxoplasma infection indicated by a low avidity of specific IgG. J Infect Dis 1989; 159: 736-740.
9 Camargo ME, da Silva SM, Leser PG, Granato CH. Avidity of specific IgG antibodies as markers of recent primary infection caused by Toxoplasma gondii. Rev Inst Med Trop Sao Paulo 1991; 33: 213-218.
10 Hedman K, Seppälä I. Recent rubella virus infection indicated by a low avidity of specific IgG. J Clin Immunol 1988; 8: 214-221.
11 Flori P, Bellete B, Crampe C, Maudry A, Patural H, Chauleur C, Hafid J, Raberin H, Tran Manh Sung R. A technique for dating toxoplasmosis in pregnancy and comparison with the Vidas anti-Toxoplasma IgG avidity test. Clin Microbiol Infect 2008; 14: 242-249.
12 Paul M. Immunoglobulin G avidity in diagnosis of toxoplasmic lymphadenopathy and ocular toxoplasmosis. Clin Diagn Lab Immunol 1999; 6: 514-518.
13 Joynson DH, Payne RA, Rawal BK. Potential role of IgG avidity for diagnosing toxoplasmosis. J Clin Pathol 1990; 43: 1032-1033.
14 Montoya JG, Huffman HB, Remington JS. Evaluation of the immunoglobulin G avidity test for diagnosis of toxoplasmic lymphadenopathy. J Clin Microbiol 2004; 42: 4627-4631.
15 Nishikawa A, Yamada H, Yamamoto T, Mizue Y, Akashi Y, Hayashi T, Nihei T, Nishiwaki M, Nishihira J. A case of congenital toxoplasmosis whose mother demonstrated serum low IgG avidity and positive tests for multiplex-nested PCR in the amniotic fluid. J Obstet Gynaecol Res 2009; 35: 372-378.
16 Press C, Montoya JG, Remington JS. Use of a single serum sample for diagnosis of acute toxoplasmosis in pregnant women and other adults. J Clin Microbiol 2005; 43: 3481-3483.
17 Remington JS, Thulliez P, Montoya JG. Recent developments for diagnosis of toxoplasmosis. J Clin Microbiol 2004; 42: 941-945.
18 Mechain B, Garin YJ, Robert-Gangneux F, Dupouy-Camet J, Derouin F. Lack of utility of specific immunoglobulin G antibody avidity for serodiagnosis of reactivated toxoplasmosis in immunocompromised patients. Clin Diagn Lab Immunol 2000; 7: 703-705.