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http://dx.doi.org/10.3347/kjp.2012.50.1.15

A Recombinant $Plasmodium$ $vivax$ Apical Membrane Antigen-1 to Detect Human Infection in Iran  

Haghi, Afsaneh Motevalli (Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences)
Khoramizade, Mohammad Reza (Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences)
Nateghpour, Mehdi (Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences)
Mohebali, Mehdi (Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences)
Edrissian, Gholam Hossein (Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences)
Eshraghian, Mohammad Reza (Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences)
Sepehrizadeh, Zargham (Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences)
Publication Information
Parasites, Hosts and Diseases / v.50, no.1, 2012 , pp. 15-21 More about this Journal
Abstract
In Iran, $Plasmodium$ $vivax$ is responsible for more than 80% of the infected cases of malaria per year. Control interventions for vivax malaria in humans rely mainly on developed diagnostic methods. Recombinant $P.$ $vivax$ apical membrane antigen-1 (rPvAMA-1) has been reported to achieve designing rapid, sensitive, and specific molecular diagnosis. This study aimed to perform isolation and expression of a rPvAMA-1, derived from Iranian patients residing in an endemic area. Then, the diagnostic efficiency of the characterized Iranian PvAMA-1 was assessed using an indirect ELISA method. For this purpose, a partial region of AMA-1 gene was amplified, cloned, and expressed in pET32a plasmid. The recombinant $His-tagged$ protein was purified and used to coat the ELISA plate. Antibody detection was assessed by indirect ELISA using rPvAMA-1. The validity of the ELISA method for detection of anti-$P.$ $vivax$ antibodies in the field was compared to light microscopy on 84 confirmed $P.$ $vivax$ patients and compared to 84 non-$P.$ $vivax$ infected individuals. The ELISA cut-off value was calculated as the mean+2SD of OD values of the people living in malaria endemic areas from a south part of Iran. We found a cut-off point of OD=0.311 that showed the best correlation between the sera confirmed with $P.$ $vivax$ infection and healthy control sera. A sensitivity of 81.0% and specificity of 84.5% were found at this cut off titer. A good degree of statistical agreement was found between ELISA using rPvAMA-1 and light microscopy (0.827) by Kappa analysis.
Keywords
Plasmodium vivax; AMA-1; recombinant antigen; ELISA; microscopy; Iran;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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