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http://dx.doi.org/10.3347/kjp.2011.49.2.139

Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea  

Kim, Jung (Department of Parasitology and Genetics, Kosin University College of Medicine)
Jo, Jin-Ok (Department of Parasitology and Genetics, Kosin University College of Medicine)
Cho, Seon-Hee (Department of Parasitology, Pusan National University School of Medicine)
Cho, Min-Kyoung (Department of Parasitology, Pusan National University School of Medicine)
Yu, Hak-Sun (Department of Parasitology, Pusan National University School of Medicine)
Cha, Hee-Jae (Department of Parasitology and Genetics, Kosin University College of Medicine)
Ock, Mee-Sun (Department of Parasitology and Genetics, Kosin University College of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.49, no.2, 2011 , pp. 139-144 More about this Journal
Abstract
The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Susan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of antibody positivity was 5.0% and 6.6% with ELISA against crude extracts and ESP, respectively. It was also revealed that infection occurred throughout all age groups and higher in females than in males. A specific protein band of 130 kDa was detected from 10 patients with western blot analysis against crude extract and ESP among those who showed positive results by ELISA. Our study showed for the first time the seroprevalence of anisakiasis in Korea. The allergen of 130 kDa can be a candidate for serologic diagnosis of anisakiasis.
Keywords
Anisakis simplex; seroepidemiology; excretory-secretory product; crude extract; ELISA; western blotting;
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