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http://dx.doi.org/10.3347/kjp.2011.49.2.109

Pro-inflammatory Cytokine Expression of Spleen Dendritic Cells in Mouse Toxoplasmosis  

Nam, Ho-Woo (Department of Parasitology, College of Medicine, Catholic University of Korea)
Ahn, Hye-Jin (Department of Parasitology, College of Medicine, Catholic University of Korea)
Yang, Hyun-Jong (Department of Parasitology, School of Medicine, Ewha Womans University)
Publication Information
Parasites, Hosts and Diseases / v.49, no.2, 2011 , pp. 109-114 More about this Journal
Abstract
Dendritic cells have been known as a member of strong innate immune cells against infectious organelles. In this study, we evaluated the cytokine expression of splenic dendritic cells in chronic mouse toxoplasmosis by tissue cyst-forming Me49 strain and demonstrated the distribution of lymphoid dendritic cells by fluorescence-activated cell sorter (FACS). Pro-inflammatory cytokines, such as IL-$1{\alpha}$, IL-$1{\beta}$, IL-6, and IL-10 increased rapidly at week 1 post-infection (PI) and peaked at week 3 PI. Serum IL-10 level followed the similar patterns. FACS analysis showed that the number of $CD8{\alpha}^+/CD11c^+$ splenic dendritic cells increased at week 1 and peaked at week 3 PI. In conclusion, mouse splenic dendritic cells showed early and rapid cytokine changes and may have important protective roles in early phases of murine toxoplasmosis.
Keywords
Toxoplasma gondii; spleen; dendritic cell; cytokine; FACS; mouse;
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