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http://dx.doi.org/10.3347/kjp.2010.48.4.319

Molecular Cloning of Plasmodium vivax Calcium-Dependent Protein Kinase 4  

Choi, Kyung-Mi (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
Kim, Jung-Yeon (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
Moon, Sung-Ung (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
Lee, Hyeong-Woo (Department of Pathology, University of Florida)
Sattabongkot, Jetsumon (Department of Entomology, Armed Forces Research Institute of Medical Science)
Na, Byoung-Kuk (Department of Parasitology and Institute of Health Sciences, Gyeongsang National University School of Medicine)
Kim, Dae-Won (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
Suh, Eun-Jung (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
Kim, Yeon-Joo (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
Cho, Shin-Hyeong (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
Lee, Ho-Sa (Institute of Global Environment and Department of Biology, Kyung Hee University)
Rhie, Ho-Gun (Institute of Global Environment and Department of Biology, Kyung Hee University)
Kim, Tong-Soo (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
Publication Information
Parasites, Hosts and Diseases / v.48, no.4, 2010 , pp. 319-324 More about this Journal
Abstract
A family of calcium-dependent protein kinases (CDPKs) is a unique enzyme which plays crucial roles in intracellular calcium signaling in plants, algae, and protozoa. CDPKs of malaria parasites are known to be key regulators for stage-specific cellular responses to calcium, a widespread secondary messenger that controls the progression of the parasite. In our study, we identified a gene encoding Plasmodium vivax CDPK4 (PvCDPK4) and characterized its molecular property and cellular localization. PvCDPK4 was a typical CDPK which had well-conserved N-terminal kinase domain and C-terminal calmodulin-like structure with 4-EF hand motifs for calcium-binding. The recombinant protein of EF hand domain of PvCDPK4 was expressed in Echerichia coli and a 34 kDa product was obtained. Immunofluorescence assay by confocal laser microscopy revealed that the protein was expressed at the mature schizont of P. vivax. The expression of PvCDPK4-EF in schizont suggests that it may participate in the proliferation or egress process in the life cycle of this parasite.
Keywords
Plasmodium vivax; calcium-dependent protein kinase; schizont; EF-hand motif;
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