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http://dx.doi.org/10.3347/kjp.2010.48.3.203

Proteomic Analysis of Haptoglobin and Amyloid A Protein Levels in Patients with Vivax Malaria  

Bahk, Young-Yil (Department of Integrated OMICS for Biomedical Sciences, Graduate School, Yonsei University)
Na, Byoung-Kuk (Department of Parasitology and Institute of Health Sciences, Gyeongsang National University School of Medicine)
Cho, Shin-Hyeong (Division of Malaria and Parasitic Diseases, National Institute of Health)
Kim, Jung-Yeon (Division of Malaria and Parasitic Diseases, National Institute of Health)
Lim, Kook-Jin (National Core Research Center for Nanomedical Technology, Yonsei University)
Kim, Tong-Soo (Department of Parasitology, Inha University School of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.48, no.3, 2010 , pp. 203-211 More about this Journal
Abstract
Advancements in the field of proteomics have provided great opportunities for the development of diagnostic and therapeutic tools against human diseases. In this study, we analyzed haptoglobin and amyloid A protein levels of vivax malaria patients with combinations of depletion of the abundant plasma proteins, 2-dimensional gel electrophoresis (2-DE), image analysis, and mass spectrometry in the plasma between normal healthy donors and vivax malaria patients. The results showed that the expression level of haptoglobin had become significantly lower or undetectable in the plasma of vivax malaria patients due to proteolytic cleavage when compared to healthy donors on 2-DE gels. Meanwhile, serum amyloid A protein was significantly increased in vivax malaria patient's plasma with high statistical values. These 2 proteins are common acute phase reactants and further large scale evaluation with a larger number of patient's will be necessary to establish the possible clinical meaning of the existential changes of these proteins in vivax malaria patients. However, our proteomic analysis suggests the feasible values of some plasma proteins, such as haptoglobin and serum amyloid A, as associating factor candidates for vivax malaria.
Keywords
Plasmodium vivax; vivax malaria; proteomics; associating factor candidate; haptoglobin; serum amyloid A;
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  • Reference
1 Sachs J, Malaney P. The economic and social burden of malaria. Nature 2002; 415: 680-685.   DOI   ScienceOn
2 Liu DH, Wang XM, Zhang LJ, Dai SW, Liu LY, Liu JF, Wu SS, Yang SY, Fu S, Xiao XY, He DC. Serum amyloid A protein: a potential biomarker correlated with clinical stage of lung cancer. Biomed Environ Sci 2007; 20: 33-40.
3 d'Eril GM, Anesi A, Maggiore M, Leoni V. Biological variation of serum amyloid A in healthy subjects. Clin Chem 2001; 47: 1498- 1499.
4 Gillespie SH, Dow C, Raynes JG, Behrens RH, Chiodini PL, Mc- Adam KPWJ. Measurement of acute phase proteins for assessing severity of Plasmodium falciparum. J Clin Pathol 1991; 44: 228- 231.   DOI
5 Anderson NL, Anderson NG. The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics 2002; 1: 845-867.   DOI   ScienceOn
6 Anderson NL, Anderson NG. Microheterogeneity of serum transferrin, haptoglobin and 2 HS glycoprotein examined by high resolution two-dimensional electrophoresis. Biochem Biophys Res Commun 1979; 8: 258-265.
7 Baumann H, Gauldie J. The acute phase response. Immunol Today 1994; 15: 74-80.   DOI   ScienceOn
8 Gabay C, Kushner I. Mechanisms of disease: acute-phase proteins and other systemic responses to inflammation. N Eng J Med 1999; 340: 448-454.   DOI   ScienceOn
9 Malle E, Sodin-Semrl S, Kovacevic A. Serum amyloid A: An acutephase protein involved in tumor pathogenesis. Cell Mol Life Sci 2009; 66: 9-26.   DOI   ScienceOn
10 Shevchenko A, Wilm M, Vorm O, Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 1996; 68: 850-858.   DOI   ScienceOn
11 Park JW, Kim S, Bahk YY. A proteomic approach for dissecting H-Ras signaling networks in NIH/3T3 mouse embryonic fibroblast cells. Proteomics 2006; 6: 2433-2443.   DOI   ScienceOn
12 Kim S, Lee YZ, Kim YS, Bahk YY. A proteomic approach for protein- profiling the oncogenic ras induced transformation (H-, K-, and N-Ras) in NIH/3T3 mouse embryonic fibroblasts. Proteomics 2008; 8: 3082-3093.   DOI   ScienceOn
13 Kristiansen M, Graversen JH, Jacobsen C, Sonne O, Hoffman HJ, Law SK, Moestrup SK. Identification of the haemoglobin scavenger receptor. Nature 2001; 409: 198-201.   DOI   ScienceOn
14 McQuire W, D'Alessandro U, Olaleye BO, Thomson MC, Langerock P, Greenwood BM, Kwiakowski D. Creactive protein and haptoglobin in the evaluation of a community-based malaria control programme. Trans R Soc Trop Med Hyg 1996; 90: 10-14.   DOI   ScienceOn
15 Mateos-Caceres PJ, Garcia-Mendez A, Lopez Farre A, Macaya C, Nunez A, Gomez J, Alonso-Orgaz S, Carrasco C, Burgos ME, de Andres R, Granizo JJ, Farre J, Rico LA. Proteomic analysis of plasma from patients during an acute coronary syndrome. J Am Coll Cardiol 2004; 44: 1578-1583.   DOI   ScienceOn
16 Hershko AY, Naparstek Y. Autoimmunity in the era of genomics and proteomics. Autoimmun Rev 2006; 5: 230-233.   DOI   ScienceOn
17 Albitar M, Potts SJ, Giles FJ, O'Brien S, Keating M, Thomas D, Clarke C, Jilani I, Aguilar C, Estey E, Kantarjian H. Proteomicbased prediction of clinical behavior in adult acute lymphoblastic leukemia. Cancer 2006; 106: 1587-1594.   DOI   ScienceOn
18 Hudelist G, Singer CF, Pischinger KI, Kaserer K, Manavi M, Kubista E, Czerwenka KF. Proteomic analysis in human breast cancer: identification of a characteristic protein expression profile of malignant breast epithelium. Proteomics 2006; 6: 1989-2002.   DOI   ScienceOn
19 Yan JX, Wait R, Berkelman T, Harry RA. A modified silver staining protocol for visualization of proteins compatible with matrixassisted laser desorption/ionization and electrospray ionization mass spectrometry. Electrophoresis 2000; 21: 3666-3672.   DOI   ScienceOn
20 Hemingway J, Bates I. Malaria: past problems and future prospects. EMBO Reports 2003; 4: S29-S31.   DOI   ScienceOn
21 Galinski MR, Barnwell JW. Plasmodium vivax merozoite invasion of reticulocytes and considera-tions for malaria vaccine development. Parasitol Today 1996; 12: 20-29.   DOI   ScienceOn
22 Richardson DC, Ciach M, Zhong KJY, Crandall I, Kain KC. Evaluation of the Markromed dipstick assay versus PCR for diagnosis of Plasmodium falciparum malaria in returned travelers. J Clin Microbiol 2002; 40: 4528-4530.
23 Snounou G, Viriyakosol S, Zhu XP, Jerra W, Pinheiro L, Rosaria VE, Thaithong V, Brown KN. High sensitivity of detection of human malaria parasites by the use of nest polymerase chain reaction. Mol Biochem Parasitol 1993; 61: 315-320.   DOI   ScienceOn
24 Jacobs JM, Adkins JN, Qian WJ, Liu T, Shen Y, Camp DG 2nd, Smith RD. Utilizing human blood plasma for proteomic biomarker discovery. J Proteome Res 2005; 4: 1073-1085.   DOI   ScienceOn