Browse > Article
http://dx.doi.org/10.3347/kjp.2008.46.3.145

Suppressed Production of Pro-inflammatory Cytokines by LPS-Activated Macrophages after Treatment with Toxoplasma gondii Lysate  

Lee, Eun-Jung (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine)
Heo, Yoo-Mi (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine)
Choi, Jong-Hak (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine)
Song, Hyun-Ouk (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine)
Ryu, Jae-Sook (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine)
Ahn, Myoung-Hee (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.46, no.3, 2008 , pp. 145-151 More about this Journal
Abstract
During Toxoplasma gondii infection, macrophages, dendritic cells, and neutrophils are important sources of pro-inflammatory cytokines from the host. To counteract the pro-inflammatory activities, T. gondii is known to have several mechanisms inducing down-regulation of the host immunity. In the present study, we analyzed the production of pro- and anti-inflammatory cytokines from a human myelomonocytic cell line, THP-1 cells, in response to treatment with T. gondii lysate or lipopolysaccharide (LPS). Treatment of THP-1 cells with LPS induced production of IL-12, TNF-$\alpha$, IL-8, and IL-10. Co-treatment of THP-1 cells with T. gondii lysate inhibited the LPS-induced IL-12, IL-8 and TNF-$\alpha$ expression, but increased the level of IL-10 synergistically. IL-12 and IL-10 production was down-regulated by anti-human toll-like receptor (TLR)-2 and TLR4 antibodies. T. gondii lysate triggered nuclear factor (NF)-${\kappa}B$-dependent IL-8 expression in HEK293 cells transfected with TLR2. It is suggested that immunosuppression induced by T. gondii lysate treatment might occur via TLR2-mediated NF-${\kappa}B$ activation.
Keywords
Toxoplasma gondii; LPS; macrophage; cytokine; toll-like receptor; NF-${\kappa}B$;
Citations & Related Records

Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
연도 인용수 순위
1 Buzoni-Gatel D, Werts C. Toxoplasma gondii and subversion of the immune system. Trends Parasitol 2006; 22: 448-452   DOI   ScienceOn
2 Belloni A, Villenu I, Gomez JE, Polloux H, Bonhomme A, Guenounoh M, Pinon JM, Aubert D. Regulation of tumor necrosis factor alpha and its specific receptors during Toxoplasma gondii infection in human monocytic cells. Parasitol Res 2003; 89: 207- 213   PUBMED
3 Belloni A, Aubert D, Gomez-Marin JE, Naour RL, Bonhomme A, Guenounou M, Pinon JM. Involvement of tumor necrosis factorduring infection of human monocytic cells by Toxoplasma gondii. Parasitol Res 2000; 86: 406-412   DOI
4 Kim JM, Oh YK, Kim YJ, Cho SJ, Ahn MH, Cho YJ. Nuclear factor- kappa B plays a major role in the regulation of chemokine expression of HeLa cells in response to Toxoplasma gondii infection. Parasitol Res 2001; 87: 758-763   DOI
5 Akira S, Sato S. Toll-like receptors and their signaling mechanisms. Scand J Infect Dis 2003; 35: 555-562   DOI   ScienceOn
6 Urematsu S, Akira S. Toll-like receptor and innate immunity. J Mol Med 2006; 84: 712-725   DOI
7 Yarovinsky F, Sher A. Toll-like receptor recognition of Toxoplasma gondii. Int J Parasitol 2006; 36: 255-259   DOI   ScienceOn
8 Campos MA, Almeida IC, Takeuchi O, Akira S, Valente EP, Procopio DO, Travassos LR, Smith JA, Golenbock DT, Gazzinelli RT. Activation of Toll-like receptor-2 by glycosylphosphatidyl-inositol anchors from a protozoan parasite. J Immunol 2001; 167: 416-423   DOI   PUBMED
9 Furuta T, Kikuchi T, Akira S, Watanabe N, Yoshikawa Y. Roles of the small intestine for induction of toll-like receptor 4-mediated innate resistance in naturally acquired murine toxoplasmosis. Int Immunol 2006; 18: 1655-1662   DOI   ScienceOn
10 Yarovinsky F, Zhang D, Ansersen JF, Bannenberg GL, Serhan CN, Hayden MS, Hiehy S, Sutterwala FS, Flavell RA, Ghosh S, Sher A. TLR11 activation of dendritic cells by a protozoan profilin- like protein. Science 2005; 308: 1626-1629   DOI   ScienceOn
11 Maldonado-Bernal C, Kirschning C, Rosenstein Y, Rocha LM, Rios-Sarabia N, Espinosa-Cantellno M, Becker I, Estrada I, Salazar- Gonzalesz RM, Lopez-Macias C, Wanga H, Sanchez J, Isibasi A. The innate immune response to Entamoeba histolytica lipopeptidophosphoglycan is mediated by toll-like receptors 2 and 4. Parasite Immunol 2005; 27: 127-137   DOI   ScienceOn
12 Debierre-Grockiego F, Campos MA, Azzouz N, Schmidt J, Bieker U, Resende MG, Mansur DS, Weingart R, Schmidt RR, Golenbock DT, Gazzinelli RT, Schwarz RT. Activation of TLR2 and TLR4 by glycosylphosphatidylinositols derived from Toxoplasma gondii. J Immunol 2007; 179: 1129-1137   DOI
13 Zanin-Zhorov A, Tal-Lapidot G, Cahalon L, Cohen-Sfady M, Pevsner- Fischer M, Lider O, Cohen IR. Cutting edge: T cells respond to lipopolysaccharide innately via TLR4 signaling. J Immunol 2007; 179: 41-44   DOI
14 Mun HS, Aosai F, Norose K, Chen M, Piao L, Takeuchi O, Akira S, Ishikura H, Yano A. TLR2 as an essential molecule for protective immunity against Toxoplasma gondii infection. Int Immunol 2003; 15: 1081-1087   DOI   ScienceOn
15 Lang C, Gross U, Luder CG. Subversion of innate and adaptive immune responses by Toxoplasma gondii. Parasitol Res 2007; 100: 191-203   DOI
16 Manson N, Aliberti J, Caamano JC, Liou H, Hunter CA. Identification of c-Rel-dependent and -independent pathways of IL-12 production during infections and inflammatory stimuli. J Immunol 2002; 168: 2590-2594   DOI   PUBMED
17 Langermans JA, Nibbering PH, Van Vuren-Van Der Halst ME, Van Furth R. Transforming growth factor-beta suppresses interferon- gamma-induced toxoplasmastatic activity in murine macrophages by inhibition of tumor necrosis factor- alpha production. Parasite Immunol 2001; 23: 169-175   DOI   ScienceOn
18 Aldebert D, Durand F, Mercier C, Brenier-Pinchart M, Cesbron- Delauw M, Pellowx H. Toxoplasma gondii triggers secretion of interleukin- 12 but low level of interleukin-10 from the THP-1 human monocyte cell line. Cytokine 2007; 206-211   PUBMED
19 Butcher BA, Kim L, Johnson PF, Denkers EY. Toxoplasma gondii tachyzoites inhibit proinflammatory cytokine induction in infected macrophages by preventing nuclear translocation of the transcription factor NF-$\kappa$B. J Immunol 2001; 167: 2193-2201   DOI   PUBMED
20 Buzoni-Gatel D, Werts C. Toxoplasma gondii and subversion of the immune system. Trends Parasitol 2006; 22: 448-452   DOI   ScienceOn
21 Ryu JS, Kang JH, Jung SY, Shin MH, Kim JM, Park H, Min DY. Production of Interleukin-8 by human neutrophils stimulated with Trichomonas vaginalis. Infect Immun 2004; 72: 1326-1332   DOI
22 Zimmermann S, Murray PJ, Heeg K, Dalpke AH. Induction of suppressor of cytokine signaling-1 by Toxoplasma gondii contributes to immune evasion in macrophages by blocking IFNsignaling. J Immunol 2006; 176: 1840-1847   DOI   PUBMED
23 Lin W, Karin M. A cytokine-mediated link between innate immunity, inflammation, and cancer. J Clin Invest 2007; 117: 1175-1183   DOI   ScienceOn
24 Butcher BA, Kim L, Panopoulos AD, Watowich SS, Murray PJ, Denkers EY. Cutting edge: IL-10-independent STAT3 activation by Toxoplasma gondii mediates suppression of IL-12 and TNF-$\alpha$ in host macrophages. J Immunol 2005; 174: 3148-3152   DOI   PUBMED
25 Del Rio L, Butcher BA, Bennouna S, Hieny S, Sher A, Denkers EY. Toxoplasma gondii triggers myeloid differentiation factor 88- dependent IL-12 and chemokine ligand 2 (monocyte chemoattractant protein 1) responses using distinct parasite molecules and host receptors. J Immunol 2004; 172: 6954-6960   DOI   PUBMED
26 Friedland JS, Shattock RJ, Johnson JD, Remick DG, Holliman RE, Griffin GE. Differential cytokine gene expression and secretion after phagocytosis by a human monocytic cell line of Toxoplasma gondii compared with Mycobacterium tuberculosis. 1993; 91: 282- 286
27 Lee CW, Bennouna S, Denkers EY. Screening for Toxoplasma gondii-regulated transcriptional responses in lipopolysaccharideactivated macrophages. Infect Immun 2006; 74: 1916-1923   DOI   ScienceOn
28 Tapping RI, Akashi S, Miyake K, Godowski PJ, Tobias PS. Tolllike receptor 4, but not toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides. J Immunol 2004; 165: 5780-5787
29 Deckert-Schluter M, Buck C, Weiner D, Kaefer N, Rang A, Hof H, Wiestler OD, Schluter D. Interleukin-10 down regulates the intracerebral immune response in chronic Toxoplasma encephalitis. J Neuroimmunol 1997; 76: 167-176   DOI   ScienceOn