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Double-stranded RNA virus in Korean Isolate IH-2 of Trichomonas vaginalis  

Kim, Jong-Wook (Department of Biochemistry, Inha Research Institute for Medical Sciences, College of Medicine, Inha University)
Chung, Pyung-Rim (Department of Parasitology College of Medicine, Inha University)
Hwang, Myung-Ki (Department of Parasitology College of Medicine, Inha University)
Choi, Eun-Young (Department of Biochemistry, College of Medicine, Inha University)
Publication Information
Parasites, Hosts and Diseases / v.45, no.2, 2007 , pp. 87-94 More about this Journal
Abstract
In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slip-page heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV If-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.
Keywords
Trichomonas vaginalis virus; TVV; dsRNA-dependent RNA polymerase; Totiviridae; ribosomal frameshift;
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1 Alderete JF, Kasmala L, Metcalfe E, Garza GE (1986) Phenotypic variation and diversity among Trichomonas vaginalis isolates and correlation of phenotype with trichomonal virulence determinants. Infect Immun 53: 285- 293   PUBMED
2 Benchimol M, Chang TH, Alderete JF (2002a) Visualization of new virus-like-particles in Trichomonas vaginalis. Tissue Cell 34: 406-415   DOI   ScienceOn
3 ICTVdB Management (2006) 00.075.0.02. Giardia virus In ICTVdB - The Universal Virus Database, version 4, Buchen-Osmond C (ed.). Columbia University, New York, USA
4 Su HM, Tai JH (1996) Genomic organization and sequence conservation in Type I Trichomonas vaginalis viruses. Virology 222: 470-473   DOI   ScienceOn
5 Wang AL, Wang CC (1985) A linear double-stranded RNA in Trichomonas vaginalis. J Biol Chem 260: 3697-3702   PUBMED
6 Wang AL, Wang CC, Alderete JF (1987) Trichomonas vaginalis phenotypic variation occurs only among Trichomonads infected with the double-stranded RNA virus. J Exp Med 166: 142-150   DOI
7 Wang AL, Yang HM, Shen KA, Wang CC (1993) Giardiavirus double-stranded RNA genome encodes a capsid polypeptide and a gag-pol-like fusion protein by a translation frameshift. Proc Natl Acad Sci USA 90: 8595- 8599
8 World Health Organization (2001) Global Prevalence and Incidence of Selected Curable Sexually Transmitted Infections: Overview and Estimates. HIV/AIDS Publications, WHO, Geneva, Switzerland
9 Scheffter SM, Ro YT, Chung LK, Patterson JL (1995) The complete sequence of Leishmania RNA virus LRV2-1, a virus of an Old World parasite strain. Virology 212: 84- 90   DOI   ScienceOn
10 Kaydos SC, Swygard H, Wise SL, Sena AC, Leone PA, Miller WC, Cohen MS, Hobbs MM (2002) Development and validation of a PCR-based enzyme-linked immunosorbent assay with urine for use in clinical research settings to detect Trichimonas vaginalis in women. J Clin Microbiol 40: 89-95   DOI
11 Wang AL, Wang CC (1986) The Double-stranded RNA in Trichomonas vaginalis may be originated from virus-like particles. Proc Natl Acad Sci USA 83: 7956-7960
12 Icho T, Wickner RB (1989) The double-stranded RNA genome of yeast virus L-A encodes its own putative RNA polymerase by fusing two open reading frames. J Biol Chem 264: 6716-6723   PUBMED
13 Scheffter S, Widmer G, Patterson JL (1994) Complete sequence of Leishmania RNA virus 1-4 and identification of conserved sequences. Virology 199: 479-483   DOI   ScienceOn
14 Benchimol M, Monteiro SP, Chang TH, Alderete JF (2002c) Virus in Trichomonas-an ultrastructural study. Parasitol Int 51: 293-298
15 Bessarab IN, Liu HW, Ip CF, Tai JH (2000) The complete sequence of a Type II Trichomonas vaginalis virus. Virology 267: 350-359   DOI   ScienceOn
16 Alderete JF, Neale KA (1989) Relatedness of structures of a major immunogen in Trichomonas vaginalis. Infect Immun 57: 1849-1853   PUBMED
17 Khoshnan A, Alderete JF (1994) Trichomonas vaginalis with a double-stranded RNA virus has upregulated levels of phenotypically variable immunogen mRNA. J Virol 68: 4035-4038   PUBMED
18 Kim JW, Bozarth RF (1985) Intergeneric occurrence of related fungal viruses: the Aspergillus ochraceous virus complex and its relationship to the Penicillium stoloniferum virus S. J Gen Virol 66: 1991-2002   DOI
19 Lawing LF, Hedges SR, Schwebke JR (2000) Detection of trichomonosis in vaginal and urine specimens from woman by culture and PCR. J Clin Microbiol 38: 3585- Kim et al.: Double-stranded RNA virus of T. vaginalis 93 3588
20 Stuart KD, Weeks R, Guilbride L, Myler PJ (1992) Molecular organization of Leishmania RNA virus 1. Proc Natl Acad Sci USA 89: 8596-8600
21 Bruenn JA (1993) A closely related group of RNA-dependent RNA polymerases from double-stranded RNA viruses. Nucleic Acids Res 21: 5667-5669   DOI
22 Wendel KA, Rompalo AM, Erbelding EJ, Chang TH, Alderete JF (2002) Double-stranded RNA viral infections of Trichomonas vaginalis infecting patients attending a sexually transmitted disease clinic. J Infec Dis 186: 558-561   DOI   ScienceOn
23 Honigberg BM, Livingston MC, Frost JK (1966) Pathogenicity of fresh isolates of Trichomonas vaginalis: 'The Mouse Assay' versus clinical and pathological findings. Acta Cytol 10: 353-361   PUBMED
24 Poch O, Sauvaget I, Delarue M, Tordo N (1989) Identification of four conserved motifs among RNAdependent polymerase encoding elements. EMBO J 8: 3867-3874   PUBMED
25 Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position- specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680   DOI
26 Benchimol M, Chang TH, Alderete JF (2002b) Trichomonas vaginalis: observation of coexistence of multiple viruses in the same isolate. FEMS Microbiol Lett 215: 197-201   DOI
27 Diamond LS (1957) The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol 43: 488-490   DOI   ScienceOn
28 Khoshnan A, Alderete JF (1993) Multiple double-stranded RNA segments are associated with virus particles infecting Trichomonas vaginalis. J Virol 67: 6950-6955   PUBMED
29 Kim JW, Kim SY, Kim KM (2003) Genome organization and expression of the Penicillium stoloniferum virus S. 27. Virus Genes 27: 249-256   DOI   ScienceOn
30 Alderete JF (1999) The Trichomonas vaginalis phenotypically varying P270 immunogen is highly conserved except for numbers of repeated elements. Microb Pathogen 27: 93- 104   DOI   ScienceOn
31 Frohman MA, Dush MK, Martin GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85: 8998-9002
32 Tai JH, Ip CF (1995) The cDNA sequence of Trichomonas vaginalis virus-T1 double-stranded RNA. Virology 206: 773-776   DOI   ScienceOn
33 Dinman JD, Icho T, Wickner RB (1991) A -1 ribosomal frameshift in a double-stranded RNA virus of yeast forms a gag-pol fusion protein. Proc Natl Acad Sci USA 88: 174-178
34 Alderete JF, Wendel KA, Rompalo AM, Erbelding EJ, Benchimol M, Chang TH (2003) Trichomonas vaginalis: evaluating capsid proteins of dsRNA viruses and the dsRNA virus within patients attending a sexually transmitted disease clinic. Exp Parasitol 103: 44-50   DOI   ScienceOn