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Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear  

Lee, Jun-Joo (Department of Parasitology, School of Medicine, Pusan National University)
Jeong, Hae-Jin (Department of Parasitology, School of Medicine, Pusan National University)
Lee, Ji-Eun (Department of Ophthalmology, School of Medicine, Pusan National University)
Lee, Jong-Soo (Department of Ophthalmology, School of Medicine, Pusan National University)
Xuan, Ying-Hua (Department of Parasitology, School of Medicine, Kyungpook National University)
Kong, Hyun-Hee (Department of Parasitology, School of Medicine, Kyungpook National University)
Chung, Dong-Il (Department of Parasitology, School of Medicine, Kyungpook National University)
Ock, Mee-Sun (Department of Parasitology, Kosin Medical College)
Yu, Hak-Sun (Department of Parasitology, School of Medicine, Pusan National University)
Publication Information
Parasites, Hosts and Diseases / v.44, no.4, 2006 , pp. 313-320 More about this Journal
Abstract
In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.
Keywords
Acanthamoeba castelianii Ma; orthokeratology; amoebic keratitis; polyhexamethylene biguanide (PHMB); 18S rDNA sequence analysis;
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