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Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis  

Kim, Kwang-Sig (Department of General Surgery, Cheju National University Hospital)
Yang, Hyun-Jong (Department of Parasitology, College of Medicine, Ewha Womans University)
Chung, Young-Bae (Department of Parasitology and Institute of Medical Science, College of Medicine, Cheju National University)
Publication Information
Parasites, Hosts and Diseases / v.41, no.2, 2003 , pp. 121-123 More about this Journal
Abstract
This study was designed to detect and evaluate an antigenicity of low molecular weight proteins of Fasciola hepatica in fascioliasis. Low molecular weight protein of F. hepatica was purified by ammonium sulfate precipitation and Sephacryl S-100 HR gel filtration. The protein obtained was estimated to be 8 kDa on 7.5-15% gradient sodium dodecyl sulfate gel electrophoresis. Immunoblotting studies showed that the 8 kDa protein reacted with human fascioliasis sera, but not other trematodiasis sera. This result suggests that the 8 kDa protein of F. hepatica is one of diagnostic antigens in human fascioliasis without cross-reaction with other human trematodiasis.
Keywords
Fasciola hepatica; low molecular protein;
Citations & Related Records

Times Cited By SCOPUS : 6
연도 인용수 순위
1 Chung YB, Lee M, Yang HJ, et al. (2002) Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis. Korean J Parasitol 40: 83-88.
2 O'Neill SM, Parkinson M, Strauss W, Angles R, Dalton JP (1998) Immunodiagnosis of Fasciola hepatica infection (fasciliasis) in a human population in the Bolivian Altiplano using purified cathepsin L cysteine proteinase. Am J Trop Med Hyg 58: 417-423.
3 Hillyer GV (1998) Immunodiagnosis of human and animal fasciolosis. In Fasciolosis, Dalton JP ed., pp 435-448, CABI Publishing, Wallingford, Oxon, UK.
4 Beaver PC, Jung RC, Cupp EW (1984) Cinical Parasitology. 9th ed. pp. 451-458, Lea & Febiger, Philadephia, USA.
5 O'Neill SM, Parkinson M, Dowd AJ, Strauss W, Angles R, Dalton JP (1999) Short report: Immunodiagnosis of human fascioliasis using recombinant Fasciola hepatica cathepsin L1 cysteine proteinase. Am J Trop Med Hyg 60:749-751.