Browse > Article

A riboprinting scheme for identification of unknown Acanthamoeba isolates at species level  

Kong, Hyun-Hee (Department of Parasitology, Kyungpook National University School of Medicine)
Chung, Dong-Il (Department of Parasitology, Kyungpook National University School of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.40, no.1, 2002 , pp. 25-31 More about this Journal
Abstract
We describe a riboprinting scheme for identification of unknown Acanthamoeba isolates at the species level. It involved the use of PCR-RFLP of small subunit ribosomal RNA gene (riboprint) of 24 reference strains by 4 kinds of restriction enzymes. Seven strains in morphological group I and III were identified at species level with their unique sizes of PCR product and riboprint type by Rsa 1. Unique RFCP of 17 strains in group II by Dde I. Taq I and Hae III were classified into: (1) four taxa that were identifiable at the species level. (2) a subgroup of 4 taxa and a pair of 2 taxi that were identical with each other. and (3) a species complex of 7 taxa assigned to A. castellanii complex that were closely related. These results were consistent with those obtained by 18s rDNA sequence analysis. This approach provides an alternative to the rDNA sequencing for rapid identification of a new clinical isolate or a large number of environmental isolates of Acanthamoeba.
Keywords
Acanthamoeba; riboprinting scheme; rapid identification;
Citations & Related Records

Times Cited By SCOPUS : 1
연도 인용수 순위
1 Moura H, Wallace S, Visvesvara GS (1992) Acanthamoeba healyi n. sp. and the isoenzyme and immunoblot profiles of Acanthamoeba spp., Groups 1 and 3. J Protozool 39: 573-583
2 Singh BN (1952) Nuclear division in nine species of small free-living amoebae and its bearing on the classification of the order Amoebida. Phil Trans Roy Soc London Ser Biol Sci 236: 405-461
3 Visvesvara GS (1991) Classification of Acanthamoeba. Rev Infect Dis 13(suppl. 5): s369-s372
4 Yu HS, Hwang MY, Kim TO, et al. (1999) Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene. Korean J Parasitol 37: 181-188
5 Reich K (1933) Studien er die Bodenprotozoen palstinos. Arch Protistenkd 79: 76-98
6 Neff R (1957) Purification, axenic cultivation and description of a soil amoeba, Acanthamoeba sp. J Protozool 4: 176-182
7 Jones DB, Visvesvara GS, Robinson NM (1975) Acanthamoeba polyphaga keratitis and Acanthamoeba uveitis associated with fatal meningoencephalitis. Trans Ophthalmol Soc UK 95: 221-232
8 Ledee DR, Hay J, Byers TJ, et al. (1996) Acanthamoeba griffini Molecular characterization of a new corneal pathogen. Invest Ophthalmol Vis Sci 37: 544-550
9 Kong HH, Chung DI (1996) PCR and RFLP variation of conserved region of small subunit ribosomal DNA among Acanthamoeba isolates assigned to either A. castellanii or A. polyphaga. Korean J Parasitol 34: 127-134
10 Lewis EJ, Sawyer TK (1979) Acanthamoeba tubiashi n. sp. a new species of fresh-water Amoebida (Acanthamoebidae). Trans Am Microsc Soc 98: 543-549
11 Stratford MP, Griffiths AJ (1978) Variations in the properties and morphology of cysts of Acanthamoeba castellanii. J Gen Microbiol 108: 33-37
12 Chung DI, Yu HS, Hwang MY, et al. (1998) Subgenus classification of Acanthamoeba by riboprinting. Korean J Parasitol 36: 69-80
13 Pussard M, Pons R (1977) Morphologie de la paroi kystique et taxonomie du genre Acanthamoeba (Protozoa, Amoebida). Protistologica 13: 557-598
14 Byers TJ, Hugo ER, Stewart VJ (1990) Genes of Acanthamoeba: DNA, RNA and protein sequences (A Review). J Protozool 37: s17-s25
15 De Jonckheere (1983) Isoenzyme and total protein analysis by agarose isoelectric focusing, and toxonomy of the genus Acanthamoeba. J Protozool 30: 701-706
16 Gast RJ, LeDee D, Fuerst PA, Byers TJ (1996) Subgenus systematics of Acanthamoeba: Four nuclear 18S rDNA sequence types. J Euk Microbiol 43: 498-504
17 Sawyer TK, Visvesvara GS, Harke BA (1977) Pathogenic amoebas from brackish and ocean sediments, with a description of Acanthamoeba hatchetti, n. sp. Science 196: 1324-1325
18 Nagington J, Watson PG, Playfair TJ, McGill J, Jones BR (1974) Amebic infection of the eye. Lancet ii: 1537-1540
19 Page FC (1967) Re-definition of the genus Acanthamoeba with descriptions of three species. J Protozool 14: 709-724
20 Sawyer TK (1971) Acanthamoeba griffini, a new species of marine amoeba. J Protozool 18: 650-654
21 Molet B, Ermolieff-Braum (1976) Description d’une amibe d’deau douce: Acanthamoeba lenticulata n. sp. (Amoebida). Protistologica 12: 571-576
22 Costas M, Griffiths AJ (1986) Physiological characterization of Acanthameoba strains. J Protozool 33: 304-309
23 Douglas M (1930) Notes on the classification of the amoeba found by Castellani in cultures of a yeast-like fungus. J Trop Med London 33: 258-259
24 Singh BN, Das SR (1970) Studies on pathogenic and nonpathogenic small free-living amoebae and the bearing of nuclear division on the classification of the order Amoebida. Phil Trans Roy Soc London Ser Biol Sci 259: 435-476
25 Kong HH, Park JH, Chung DI (1995) Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to Acanthamoeba polyphaga. Korean J Parasitol 33: 331-340
26 Ray DL, Hayes RE (1954) Hartmannella astronyxis: a new species of free-living amoeba. Cytology and life cycle. J Morphol 95: 159-188
27 Chung DI, Kong HH, Yu HS, Oh YM, Yee ST, Lim YJ (1996) Biochemical and molecular characterization of a strain KA/S2 of Acanthamoeba castellanii isolated from Korean soil. Korean J Parasitol 34: 79-85
28 Sawyer TK, Nerad TA, Lewis EJ, McLaughlin SM (1993) Acanthamoeba stevensoni n. sp. (Protozoa: Amoebida) from sewagecontaminated shellfish beds in Raritan bay, New York. J Protozool 40: 742-746
29 Jacobson LM, Band RN (1987) Genetic heterogeneity in a natural population of Acanthamoeba polyphaga from soil, an isoenzyme analysis. J Protozool 34: 83-86
30 Ma PE, Willaert KB, Stevens AR (1981) A case of keratitis due to Acanthamoeba in New York, and features of 10 cases. J Inf Dis 143: 662-667
31 Stothard DR, Schroeder-Diedrich JM, Awwad MH, et al. (1998) The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J Euk Microbiol 45: 45-54