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http://dx.doi.org/10.1016/j.jgr.2022.02.004

Korean Red Ginseng saponin fraction exerts anti-inflammatory effects by targeting the NF-κB and AP-1 pathways  

Lee, Jeong-Oog (Department of Aerospace Information Engineering, Bio-Inspired Aerospace Information Laboratory, Konkuk University)
Yang, Yanyan (Department of Immunology, Basic Medicine School, Qingdao University)
Tao, Yu (Department of Cardiac Ultrasound and Institute for Translational Medicine, The Affiliated Hospital of Qingdao University)
Yi, Young-Su (Department of Life Sciences, Kyonggi University)
Cho, Jae Youl (Department of Integrative Biotechnology, Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University)
Publication Information
Journal of Ginseng Research / v.46, no.3, 2022 , pp. 489-495 More about this Journal
Abstract
Background: Although ginsenosides and saponins in Korea red ginseng (KRG) shows various pharmacological roles, their roles in the inflammatory response are little known. This study investigated the anti-inflammatory role of ginsenosides identified from KRG saponin fraction (RGSF) and the potential mechanism in macrophages. Methods: The ginsenoside composition of RGSF was identified by high-performance liquid chromatography (HPLC) analysis. An anti-inflammatory effect of RGSF and its mechanisms were studied using nitric oxide (NO) and prostaglandin E2 (PGE2) production assays, mRNA expression analyses of inflammatory genes and cytokines, luciferase reporter gene assays of transcription factors, and Western blot analyses of inflammatory signaling pathways using the lipopolysaccharide (LPS)-treated RAW264.7 cells. Results: HPLC analysis identified the types and amounts of various panaxadiol ginsenosides in RGSF. RGSF reduced the generation of inflammatory molecules and mRNA levels of inflammatory enzymes and cytokines in LPS-treated RAW264.7 cells. Additionally, RGSF inhibited the signaling pathways of NF-κB and AP-1 by suppressing both transcriptional factors and signaling molecules in LPS-treated RAW264.7 cells. Conclusion: RGSF contains ginsenosides that have anti-inflammatory action via restraining the NF-κB and AP-1 signaling pathways in macrophages during inflammatory responses.
Keywords
RGSF; Anti-Inflammatory; Macrophage; NF-${\kappa}B$; AP-1;
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