Browse > Article
http://dx.doi.org/10.1016/j.jgr.2017.04.005

Investigating chemical features of Panax notoginseng based on integrating HPLC fingerprinting and determination of multiconstituents by single reference standard  

Yang, Zhenzhong (Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University)
Zhu, Jieqiang (Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University)
Zhang, Han (Tianjin University of Traditional Chinese Medicine)
Fan, Xiaohui (Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University)
Publication Information
Journal of Ginseng Research / v.42, no.3, 2018 , pp. 334-342 More about this Journal
Abstract
Background: Panax notoginseng is a highly valued medicine and functional food, whose quality is considered to be influenced by the size, botanical parts, and growth environments. Methods: In this study, a HPLC method integrating fingerprinting and determination of multiconstituents by single reference standard was established and adopted to investigate the chemical profiles and active constituent contents of 215 notoginseng samples with different sizes, from different botanical parts and geographical regions. Results: Chemical differences among main root, branch root, and rotten root were not distinct, while rhizome and fibrous root could be discriminated from other parts. The notoginseng samples from Wenshan Autonomous Prefecture and cities nearby were similar, whereas samples from cities far away were not. The contents of major active constituents in main root did not correlate with the market price. Conclusion: This study provided comprehensive chemical evidence for the rational usage of different parts, sizes, and growth regions of notoginseng in practice.
Keywords
chemical profile; geographical region; HPLC fingerprinting; multivariate analysis; Panax notoginseng;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Wang D, Li H, Chen K, Zhang Y, Yang C. HPLC comparative analysis of ginsenoside saponins indifferent underground parts of Panax notoginseng. Acta Botanica Yunnanica 2005;27:685-90.
2 Hong DYQ, Lau AJ, Yeo CL, Liu XK, Yang CR, Koh HL, Hong Y. Genetic diversity and variation of saponin contents in Panax notoginseng roots from a single farm. J Agr Food Chem 2005;53:8460-7.   DOI
3 Ma J, Mi YH, Li QW, Chen L, Du LJ, He LZ, Lei M. Reduction, methylation, and translocation of arsenic in Panax notoginseng grown under field conditions in arsenic-contaminated soils. Sci Total Environ 2016;550:893-9.   DOI
4 Zhu J, Fan X, Cheng Y, Agarwal R, Moore CMV, Chen ST, Tong W. Chemometric analysis for identification of botanical raw materials for pharmaceutical use: a case study using Panax notoginseng. PLoS One 2014;9:e87462.   DOI
5 Kuang GJ, Zhou J, Yao MC, Tan QL, Qi LK, Liang JH, Li Y, Zhao ZX, Zhang L. Systematic study on QAMS method for simultaneous determination of triterpenoid saponins in Ilex pubescens by HPLC and UPLC. Anal Method 2015;7:6579-87.   DOI
6 Yao H, Shi PY, Shao Q, Fan XH. Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS. Chin Med 2011;6:8.   DOI
7 Wang XM, Wang SX, Wang JX, Guo H, Dong ZP, Chai LJ, Hu LM, Zhang Y, Wang H, Chen L. Neuroprotective effect of Xueshuantong for injection (lyophilized) in transient and permanent rat cerebral ischemia model. Evid Based Compl Alt 2015;2015:1-13.
8 Zheng YK, Miao CP, Chen HH, Huang FF, Xia YM, Chen YW, Zhao LX. Endophytic fungi harbored in Panax notoginseng: diversity and potential as biological control agents against host plant pathogens of root-rot disease. J Ginseng Res 2017;41:353-60.   DOI
9 Sun Y, Ke J, Ma N, Chen Z, Wang C, Cui X. Effects of root rot on saponin content in Panax notoginseng. J Chinese Med Mater 2004;27:79-80.
10 Bondia-Pons I, Savolainen O, Torronen R, Martinez JA, Poutanen K, Hanhineva K. Metabolic profiling of Goji berry extracts for discrimination of geographical origin by non-targeted liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Food Res Int 2014;63:132-8.   DOI
11 Qiu YQ, Lu X, Pang T, Zhu SK, Kong HW, Xu GW. Study of traditional Chinese medicine volatile oils from different geographical origins by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC $\times$ GC-TOFMS) in combination with multivariate analysis. J Pharmaceut Biomed 2007;43:1721-7.   DOI
12 Liu JJ, Wang YT, Qiu L, Yu YY, Wang CM. Saponins of Panax notoginseng: chemistry, cellular targets and therapeutic opportunities in cardiovascular diseases. Expert Opin Investig Drugs 2014;23:523-39.   DOI
13 Efferth T, Koch E. Complex interactions between phytochemicals. The multitarget therapeutic concept of phytotherapy. Curr Drug Targets 2011;12:122-32.   DOI
14 Yang ZZ, Liu W, Zhang F, Li Z, Cheng YY. Deciphering the therapeutic mechanisms of Xiao-Ke-An in treatment of type 2 diabetes in mice by a Fangjiomics approach. Acta Pharmacol Sinic 2015;36:699-707.   DOI
15 Ng TB. Pharmacological activity of sanchi ginseng (Panax notoginseng). J Pharm Pharmacol 2006;58:1007-19.   DOI
16 Peng M, Zhang T, Ding Y, Yi YX, Yang YJ, Le J. Structure-based prediction of CAD response factors of dammarane-type tetracyclic triterpenoid saponins and its application to the analysis of saponin contents in raw and processed Panax notoginseng. RSC Adv 2016;6:36987-7005.   DOI
17 Kim DH. Chemical diversity of Panax ginseng, Panax quinquifolium, and Panax notoginseng. J Ginseng Res 2012;36:1-15.   DOI
18 Wan JB, Yang FQ, Li SP, Wang YT, Cui XM. Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD. J Pharmaceut Biomed 2006;41:1596-601.   DOI
19 Wang J, Yau L, Gao W, Liu Y, Yick P, Liu L, Jiang Z. Quantitative comparison and metabolite profiling of saponins in different parts of the root of Panax notoginseng. J Agr Food Chem 2014;62:9024-34.   DOI
20 Wang L, Li Z, Shao Q, Li X, Ai N, Zhao X, Fan X. Dissecting active ingredients of Chinese medicine by content-weighted ingredientetarget network. Mol Bio-Syst 2014;10:1905-11.
21 Zheng D, Wang L, Ou X, Guo L, Hao Q, Liu D, Xiao Y. Comparison of agronomic traits of Panax notoginseng between traditional cultivated fields and new cultivated fields. China J Chinese Materia Med 2014;39:558-65.
22 Li H, He JX, Li FJ, Zhang ZJ, Li RR, Su JC, Zhang JD, Yang B. Application of NIR and MIR spectroscopy for rapid determination of antioxidant activity of Radix Scutellariae from different geographical regions. Phytochem Analysis 2016;27:73-80.   DOI
23 Zhao ZZ, Guo P, Brand E. The formation of daodi medicinal materials. J Ethnopharmacol 2012;140:476-81.   DOI
24 Dong TTX, Cui XM, Song ZH, Zhao KJ, Ji ZN, Lo CK, Tsim KWK. Chemical assessment of roots of Panax notoginseng in China: Regional and seasonal variations in its active constituents. J Agr Food Chem 2003;51:4617-23.   DOI
25 Da J, Cheng CR, Yao S, Long HL, Wang YH, Khan IA, Li YF, Wang QR, Cai LY, Jiang BH, et al. A reproducible analytical system based on the multicomponent analysis of triterpene acids in Ganoderma lucidum. Phytochemistry 2015;114:146-54.   DOI
26 Sun XT, Li L, Long GQ, Zhang GH, Meng ZG, Yang SC, Chen JW. The progress and prospect on consecutive monoculture problems of Panax notoginseng. Chinese J Ecol 2015;34:885-93.
27 Cui XM, Huang LQ, Guo LP, Liu DH. Chinese sanqi industry status and development countermeasures. China J Chinese Materia Med 2014;39:553-7.
28 Shan LM, Zhao YL, Hong W, Zhang P, Kong WJ, Liu ZG, Xiao XH. Correlation analysis between hemostatic activity and commercial grades of Panax notoginseng. Chinese Tradit Herbal Drugs 2011;42:1779-82.
29 Wang LC, Zhang WS, Liu Q, Li J, Alolga RN, Liu K, Liu BL, Li P, Qi LW. A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions. J Funct Foods 2015;16:20-7.   DOI
30 Wang D, Liao P, Zhu H, Chen K, Xu M, Zhang Y, Yang C. The processing of Panax notoginseng and the transformation of its saponin components. Food Chem 2012;132:1808-13.   DOI
31 Pan WD, Yang LX, Feng WH, Lin LM, Li C, Liu WW, Gan GF, Fan JH, Zou JG, Wang ZM, et al. Determination of five sesquiterpenoids in Xingnaojing injection by quantitative analysis of multiple components with a single marker. J Sep Sci 2015;38:3313-23.   DOI
32 Song Y, Wang Z, Zhu J, Yan L, Zhang Q, Gong M, Gao H, Feng W, Wang W. Assay of evodin, evodiamine and rutaecarpine in Fructus evodiae by QAMS. China J Chinese Materia Med 2009;34:2781-5.
33 Hou JJ, Wu WY, Da J, Yao S, Long HL, Yang Z, Cai LY, Yang M, Liu X, Jiang BH, et al. Ruggedness and robustness of conversion factors in method of simultaneous determination of multi-components with single reference standard. J Chromatogr A 2011;1218:5618-27.   DOI
34 Gao XY, Jiang Y, Lu JQ, Tu PF. One single standard substance for the determination of multiple anthraquinone derivatives in rhubarb using highperformance liquid chromatographyediode array detection. J Chromatogr A 2009;1216:2118-23.   DOI
35 Gao W, Wang R, Li D, Liu K, Chen J, Li HJ, Xu X, Li P, Yang H. Comparison of five Lonicera flowers by simultaneous determination of multi-components with single reference standard method and principal component analysis. J Pharmaceut Biomed 2016;117:345-51.   DOI
36 Wang CQ, Jia XH, Zhu S, Komatsu K, Wang X, Cai SQ. A systematic study on the influencing parameters and improvement of quantitative analysis of multicomponent with single marker method using notoginseng as research subject. Talanta 2015;134:587-95.   DOI
37 Rhule A, Navarro S, Smith JR, Shepherd DA. Panax notoginseng attenuates LPSinduced pro-inflammatory mediators in RAW264.7 cells. J Ethnopharmacol 2006;106:121-8.   DOI
38 Yang Y. Scientific substantiation of functional food health claims in China. J Nutr 2008;138. 1199S-205S.   DOI
39 Chen L, Tai WCS, Hsiao WLW. Dietary saponins from four popular herbal tea exert prebiotic-like effects on gut microbiota in C57BL/6 mice. J Funct Foods 2015;17:892-902.   DOI
40 Sun S, Wang C, Tong R, Li X, Fishbein A, Wang Q, He T, Du W, Yuan CS. Effects of steaming the root of Panax notoginseng on chemical composition and anticancer activities. Food Chem 2010;118:307-14.   DOI
41 Jia X, Wang C, Liu J, Li X, Wang X, Shang M, Cai S, Zhu S, Komatsu K. Comparative studies of saponins in 1-3-year-old main roots, fibrous roots, and rhizomes of Panax notoginseng, and identification of different parts and growth-year samples. J Nat Med 2013;67:339-49.   DOI
42 Guan H, Zhang Y, Chen Y, Yang J, Sun S. On the connections between sanchi root rot and edaphon dynamics in no-tillage sanchi-planted soil. In: International conference on computer distributed control and intelligent environmental monitoring (CDCIEM). IEEE; 2011. p. 1598-602.
43 Xia P, Guo H, Zhao H, Jiao J, Deyholos MK, Yan X, Liu Y, Liang Z. Optimal fertilizer application for Panax notoginseng and effect of soil water on root rot disease and saponin contents. J Ginseng Res 2016;40:38-46.   DOI