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http://dx.doi.org/10.1016/j.jgr.2017.01.002

Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng  

Hong, Yo Han (Department of Genetic Engineering, Sungkyunkwan University)
Kim, Donghyun (Amorepacific Research and Development Unit)
Nam, Gibaeg (Amorepacific Research and Development Unit)
Yoo, Sulgi (Department of Genetic Engineering, Sungkyunkwan University)
Han, Sang Yun (Department of Genetic Engineering, Sungkyunkwan University)
Jeong, Seong-Gu (Department of Genetic Engineering, Sungkyunkwan University)
Kim, Eunji (Department of Genetic Engineering, Sungkyunkwan University)
Jeong, Deok (Department of Genetic Engineering, Sungkyunkwan University)
Yoon, Keejung (Department of Genetic Engineering, Sungkyunkwan University)
Kim, Sunggyu (Research and Business Foundation, Sungkyunkwan University)
Park, Junseong (Amorepacific Research and Development Unit)
Cho, Jae Youl (Department of Genetic Engineering, Sungkyunkwan University)
Publication Information
Journal of Ginseng Research / v.42, no.1, 2018 , pp. 81-89 More about this Journal
Abstract
Background: BIOGF1K, a compound-K-rich fraction, has been shown to display anti-inflammatory activity. Although Panax ginseng is widely used for the prevention of photoaging events induced by UVB irradiation, the effect of BIOGF1K on photoaging has not yet been examined. In this study, we investigated the effects of BIOGF1K on UVB-induced photoaging events. Methods: We analyzed the ability of BIOGF1K to prevent UVB-induced apoptosis, enhance matrix metalloproteinase (MMP) expression, upregulate anti-inflammatory activity, reduce sirtuin 1 expression, and melanin production using reverse transcription-polymerase chain reaction, melanin content assay, tyrosinase assay, and flow cytometry. We also evaluated the effects of BIOGF1K on the activator protein-1 signaling pathway, which plays an important role in photoaging, by immunoblot analysis and luciferase reporter gene assays. Results: Treatment of UVB-irradiated NIH3T3 fibroblasts with BIOGF1K prevented UVB-induced cell death, inhibited apoptosis, suppressed morphological changes, reduced melanin secretion, restored the levels of type I procollagen and sirtuin 1, and prevented mRNA upregulation of MMP-1, MMP-2, and cyclo-oxygenase-2; these effects all occurred in a dose-dependent manner. In addition, BIOGF1K markedly reduced activator-protein-1-mediated luciferase activity and decreased the activity of mitogen-activated protein kinases (extracellular response kinase, p38, and C-Jun N-terminal kinase). Conclusion: Our results strongly suggest that BIOGF1K has anti-photoaging activity and that BIOGF1K could be used in anti-aging cosmeceutical preparations.
Keywords
anti-aging; BIOGF1K; Panax ginseng; photoaging; UVB irradiation;
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Times Cited By KSCI : 8  (Citation Analysis)
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1 Sinha RP, Hader DP. UV-induced DNA damage and repair: a review. Photochem Photobiol Sci 2002;1:705-20.
2 Jablonski NG, Chaplin G. Chaplin, Human skin pigmentation as an adaptation to UV radiation. Proc Natl Acad Sci U S A 2010;107:8962-8.   DOI
3 Tang D, Wu D, Hirao A, Lahti JM, Liu L, Mazza B, Kidd VJ, Mak TW, Ingram AJ. ERK activation mediates cell cycle arrest and apoptosis after DNA damage independently of p53. J Biol Chem 2002;277:12710-7.   DOI
4 Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol 2007;35:495-516.   DOI
5 Baumann L. How to prevent photoaging? J Invest Dermatol 2005;125:xii-xiii.
6 Xiao GH, Jeffers M, Bellacosa A, Mitsuuchi Y, Woude GFV, Testa JR. Antiapoptotic signaling by hepatocyte growth factor/Met via the phosphatidylinositol 3-kinase/Akt and mitogen-activated protein kinase pathways. Proc Natl Acad Sci U S A 2001;98:247-52.   DOI
7 Matsumura M, Tanaka N, Kuroki T, Ichihashi M, Ohba M. The $\eta$ isoform of protein kinase C inhibits UV-induced activation of caspase-3 in normal human keratinocytes. Biochem Biophys Res Commun 2003;303:350-6.   DOI
8 Cho S, Kim HH, Lee MJ, Lee S, Park C-S, Nam S-J, Han J-J, Kim J-W, Chung JH. Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo. J Lipid Res 2008;49:1235-45.   DOI
9 Kang TH, Park HM, Kim YB, Kim H, Kim N, Do JH, Kang C, Cho Y, Kim SY. Effects of red ginseng extract on UVB irradiation-induced skin aging in hairless mice. J Ethnopharmacol 2009;123:446-51.   DOI
10 Guo L, Huang ZX, Chen XW, Deng QK, Yan W, Zhou MJ, Ou CS, Ding ZH. Differential expression profiles of microRNAs in NIH3T3 cells in response to UVB irradiation. Photochem Photobiol 2009;85:765-73.   DOI
11 Brenneisen P, Sies H, Scharffetter-Kochanek K. Ultraviolet-B irradiation and matrix metalloproteinases. Ann N Y Acad Sci 2002;973:31-43.   DOI
12 Asadamongkol B, Zhang JH. The development of hyperbaric oxygen therapy for skin rejuvenation and treatment of photoaging. Med Gas Res 2014;4:1.   DOI
13 Bosch R, Philips N, Suarez-Perez JA, Juarranz A, Devmurari A, Chalensouk-Khaosaat J, Gonzalez S. Mechanisms of photoaging and cutaneous photocarcinogenesis, and photoprotective strategies with phytochemicals. Antioxidants 2015;4:248-68.   DOI
14 Lu J, Guo JH, Tu XL, Zhang C, Zhao M, Zhang QW, Gao FH. Tiron Inhibits UVBinduced AP-1 binding sites transcriptional activation on MMP-1 and MMP-3 promoters by MAPK signaling pathway in human dermal fibroblasts. PloS One 2016;11:e0159998.   DOI
15 Lee HJ, Hwang E, Park B, Zhang M, Zw Sun, Lee DG, Park SY, Yi TH. Methanol extract of bitter melon alleviates UVB-induced MMPs expression via MAP kinase and AP-1 signaling in human dermal fibroblasts in vitro. Phytother Res 2016;30:1519-26.   DOI
16 D'Mello SA, Finlay GJ, Baguley BC, Askarian-Amiri ME. Signaling pathways in melanogenesis. Int J Mol Sci 2016;17:1144.   DOI
17 Brennan M, Bhatti H, Nerusu KC, Bhagavathula N, Kang S, Fisher GJ, Varani J, Voorhees JJ. Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin. Photochem Photobiol 2003;78:43-8.   DOI
18 Jung SK, Lee KW, Kim HY, Oh MH, Byun S, Lim SH, Heo YS, Kang NJ, Bode AM, Dong Z. Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf. Biochem Pharmacol 2010;79:1455-61.   DOI
19 Chung KW, Choi YJ, Park MH, Jang EJ, Kim DH, Park BH, Yu BP, Chung HY. Molecular insights into SIRT1 protection against UVB-induced skin fibroblast senescence by suppression of oxidative stress and p53 acetylation. J Gerontol A Biol Sci Med Sci 2014;70:959-68.
20 Yan Y, Li L, Xu H, Peng S, Qu T, Wang B. Receptor interacting protein 1 involved in ultraviolet B induced NIH3T3 cell apoptosis through expression of matrix metalloproteinases and reactive oxygen species production. Chin Med J 2013;126:4327-33.
21 Ming M, Soltani K, Shea CR, Li X, He YY. Dual role of SIRT1 in UVB-induced skin tumorigenesis. Oncogene 2015;34:357-63.   DOI
22 Cao C, Lu S, Kivlin R, Wallin B, Card E, Bagdasarian A, Tamakloe T, Wj Wang, Song X, Wm Chu. SIRT1 confers protection against UVB-and H2O2-induced cell death via modulation of p53 and JNK in cultured skin keratinocytes. J Cell Mol Med 2009;13:3632-43.   DOI
23 Lee HJ, Lee JY, Song KC, Kim JH, Park JH, Chun KH, Hwang GS. Protective effect of processed Panax ginseng, sun ginseng on UVB-irradiated human skin keratinocyte and human dermal fibroblast. J Ginseng Res 2012;36:68-77.   DOI
24 Englaro W, Rezzonico R, Durand-Clement M, Lallemand D, Ortonne JP, Ballotti R. Mitogen-activated protein kinase pathway and AP-1 are activated during cAMP-induced melanogenesis in B-16 melanoma cells. J Biol Chem 1995;270:24315-20.   DOI
25 Kim JH, Baek EJ, Lee EJ, Yeom MH, Park JS, Lee KW, Kang NJ. Ginsenoside F1 attenuates hyperpigmentation in B16F10 melanoma cells by inducing dendrite retraction and activating Rho signalling. Exp Dermatol 2015;24: 150-2.   DOI
26 Choi Kt. Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng CA Meyer. Acta Pharmacol Sin 2008;29:1109-18.   DOI
27 Hossen MJ, Hong YD, Baek KS, Yoo S, Hong YH, Kim JH, Lee JO, Kim D, Park J, Cho JY. In vitro antioxidative and anti-inflammatory effects of the compound K-rich fraction BIOGF1K, prepared from Panax ginseng. J Ginseng Res 2017;41: 43-51.   DOI
28 Lee DY, Jeong YT, Jeong SC, Lee MK, Min JW, Lee JW, Kim GS, Lee SE, Ahn YS, Kang HC, Kim JH. Melanin biosynthesis inhibition effects of Ginsenoside Rb2 isolated from Panax ginseng Berry. J Microbiol Biotechnol 2015;25:2011-5.   DOI
29 Zhu L, Lu Y, Yu WG, Zhao X, Lu YH. Anti-photoageing and anti-melanogenesis activities of chrysin. Pharm Biol 2016:1-9.
30 Park SY, Shin YK, Kim HT, Kim YM, Lee DG, Hwang E, Cho BG, Yin CS, Kim KY, Yi TH. A single-center, randomized, double-blind, placebo-controlled study on the efficacy and safety of "enzyme-treated red ginseng powder complex (BG11001)" for antiwrinkle and proelasticity in individuals with healthy skin. J Ginseng Res 2016;40:260-8.   DOI
31 Baek KS, Hong YD, Kim Y, Sung NY, Yang S, Lee KM, Park JY, Park JS, Rho HS, Shin SS, Cho JY. Anti-inflammatory activity of AP-SF, a ginsenoside-enriched fraction, from Korean ginseng. J Ginseng Res 2015;39:155-61.   DOI
32 Li Z, Ryu SW, Lee J, Choi K, Kim S, Choi C. Protopanaxatirol type ginsenoside Re promotes cyclic growth of hair follicles via inhibiting transforming growth factor beta signaling cascades. Biochem Biophys Res Commun 2016;470:924-9.   DOI
33 Park SH, Seo W, Eun HS, Kim SY, Jo E, Kim MH, Choi WM, Lee JH, Shim YR, Cui CH, Kim SC, Hwang CY, Jeong WI. Protective effects of ginsenoside F2 on 12-O-tetradecanoylphorbol-13-acetate-induced skin inflammation in mice. Biochem Biophys Res Commun 2016;478:1713-9.   DOI
34 Yang Y, Yu T, Jang HJ, Byeon SE, Song SY, Lee BH, Rhee MH, Kim TW, Lee J, Hong S. In vitro and in vivo anti-inflammatory activities of Polygonum hydropiper methanol extract. J Ethnopharmacol 2012;139:616-25.   DOI
35 Hossen MJ, Baek KS, Kim E, Yang WS, Jeong D, Kim JH, Kweon DH, Yoon DH, Kim TW, Kim JH. In vivo and in vitro anti-inflammatory activities of Persicaria chinensis methanolic extract targeting Src/Syk/NF-${\kappa}B$. J Ethnopharmacol 2015;159:9-16.   DOI
36 Tavazoie SF, Alarcon C, Oskarsson T, Padua D, Wang Q, Bos PD, Gerald WL, Massague J. Endogenous human microRNAs that suppress breast cancer metastasis. Nature 2008;451:147-52.   DOI
37 Ding N, Ruth TY, Subramaniam N, Sherman MH, Wilson C, Rao R, Leblanc M, Coulter S, He M, Scott C. A vitamin D receptor/SMAD genomic circuit gates hepatic fibrotic response. Cell 2013;153:601-13.   DOI
38 Tan SY, Ang CY, Luo Z, Li P, Nguyen KT, Zhao Y. An iGlu receptor antagonist and its simultaneous use with an anticancer drug for cancer therapy. Chemistry 2015;21:6123-31.   DOI
39 Baek KS, Yi YS, Son YJ, Yoo S, Sung NY, Kim Y, Hong S, Aravinthan A, Kim JH, Cho JY. In vitro and in vivo anti-inflammatory activities of Korean Red Ginseng-derived components. J Ginseng Res 2016;40:437-44.   DOI
40 Kim MY, Cho JY. 20S-dihydroprotopanaxadiol, a ginsenoside derivative, boosts innate immune responses of monocytes and macrophages. J Ginseng Res 2013;37:293-9.   DOI
41 Starkenmann C, Luca L, Niclass Y, Praz E, Roguet D. Comparison of volatile constituents of Persicaria odorata(Lour.) Sojak (Polygonum odoratum Lour.) and Persicaria hydropiper L. Spach (Polygonum hydropiper L.). J Agric Food Chem 2006;54:3067-71.   DOI
42 Almela L, Sanchez-Munoz B, Fernandez-Lopez JA, Roca MJ, Rabe V. Liquid chromatograpic-mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different raw material. J Chromatogr A 2006;1120:221-9.   DOI
43 Won YK, Lin CB, Seiberg M, Chen N, Hu Y, Rossetti D, Saliou C, Loy CJ. Galvanic zinc-copper microparticles inhibit melanogenesis via multiple pigmentary pathways. Arch Dermatol Res 2014;306:27-35.   DOI
44 Chung I, Lee J, Park YS, Lim Y, Chang DH, Park J, Hwang JS. Inhibitory mechanism of Korean Red Ginseng on GM-CSF expression in UVB-irradiated keratinocytes. J Ginseng Res 2015;39:322-30.   DOI
45 Lee EJ, Kim JY, Oh SH. Advanced glycation end products (AGEs) promote melanogenesis through receptor for AGEs. Sci Rep 2016;6:27848.   DOI
46 Park PJ, Lee TR, Cho EG. Substance P stimulates endothelin 1 secretion via endothelin-converting enzyme 1 and promotes melanogenesis in human melanocytes. J Invest Dermatol 2015;135:551-9.   DOI
47 Bode AM, Dong Z. Mitogen-activated protein kinase activation in UV-induced signal transduction. Sci STKE 2003;2003:RE2.
48 Cho MK, Jang YP, Kim YC, Kim SG. Arctigenin, a phenylpropanoid dibenzylbutyrolactone lignan, inhibits MAP kinases and AP-1 activation via potent MKK inhibition: the role in TNF-${\alpha}$ inhibition. Int Immunopharmacol 2004;4: 1419-29.   DOI
49 Cheng Y, Ren X, Zhang Y, Patel R, Sharma A, Wu H, Robertson GP, Yan L, Rubin E, Yang JM. eEF-2 kinase dictates cross-talk between autophagy and apoptosis induced by Akt inhibition, thereby modulating cytotoxicity of novel Akt inhibitor MK-2206. Cancer Res 2011;71:2654-63.   DOI
50 Lee HJ, Hyun EA, Yoon WJ, Kim BH, Rhee MH, Kang HK, Cho JY, Yoo ES. In vitro anti-inflammatory and anti-oxidative effects of Cinnamomum camphora extracts. J Ethnopharmacol 2006;103:208-16.   DOI
51 Kubo I, Nitoda T, Ki Nihei. Effects of quercetin on mushroom tyrosinase and B16-F10 melanoma cells. Molecules 2007;12:1045-56.   DOI
52 Tanos T, Marinissen MJ, Leskow FC, Hochbaum D, Martinetto H, Gutkind JS, Coso OA. Phosphorylation of c-Fos by members of the p38 MAPK family role in the AP-1 response to UV light. J Biol Chem 2005;280:18842-52.   DOI
53 Ikehata H, Ono T. The mechanisms of UV mutagenesis. J Radiat Res 2011;52: 115-25.   DOI
54 Brash DE, Rudolph JA, Simon JA, Lin A, McKenna GJ, Baden HP, Halperin AJ, Ponten J. A role for sunlight in skin cancer: UV-induced p53 mutations in squamous cell carcinoma. Proc Natl Acad Sci U S A 1991;88:10124-8.   DOI
55 Armstrong BK, Kricker A. The epidemiology of UV induced skin cancer. J Photochem Photobiol B 2001;63:8-18.   DOI
56 Rittie L, Fisher GJ. UV-light-induced signal cascades and skin aging. Ageing Res Rev 2002;1:705-20.   DOI
57 Brown WT. Human mutations affecting aging - a review. Mech Ageing Dev 1979;9:325-36.   DOI