[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.1016/j.jgr.2016.12.013

Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells

Lee, Dahae (School of Pharmacy, Sungkyunkwan University)
Lee, Dong-Soo (Institute of Human-Environment Interface Biology, Biomedical Research Institute, Department of Dermatology, Seoul National University College of Medicine)
Jung, Kiwon (College of Pharmacy, CHA University)
Hwang, Gwi Seo (College of Korean Medicine, Gachon University)
Lee, Hye Lim (College of Korean Medicine, Gachon University)
Yamabe, Noriko (College of Korean Medicine, Gachon University)
Lee, Hae-Jeong (Department of Food and Nutrition, Gachon University)
Eom, Dae-Woon (Department of Pathology, University of Ulsan College of Medicine)
Kim, Ki Hyun (School of Pharmacy, Sungkyunkwan University)
Kang, Ki Sung (College of Korean Medicine, Gachon University)

Publication Information

Journal of Ginseng Research / v.42, no.1, 2018 , pp. 75-80 More about this Journal

Abstract

Background: The aim of the present study was to evaluate the potential protective effects of six ginsenosides (Rb1, Rb2, Rc, Rd, Rg1, and Rg3) isolated from Panax ginseng against tacrolimus (FK506)-induced apoptosis in renal proximal tubular LLC-PK1 cells. Methods: LLC-PK1 cells were treated with FK506 and ginsenosides, and cell viability was measured. Protein expressions of mitogen-activated protein kinases, caspase-3, and kidney injury molecule-1 (KIM-1) were evaluated by Western blotting analyses. The number of apoptotic cells was measured using an image-based cytometric assay. Results: Reduction in cell viability by $60{\mu}M$ FK506 was ameliorated significantly by cotreatment with ginsenosides Rg1 and Rb1. The phosphorylation of p38, extracellular signal-regulated kinases, and KIM-1, and cleavage of caspase-3, increased markedly in LLC-PK1 cells treated with FK506 and significantly decreased after cotreatment with ginsenoside Rb1. The number of apoptotic cells decreased by 6.0% after cotreatment with ginsenoside Rb1 ( $10{\mu}M$ and $50{\mu}M$ ). Conclusion: The antiapoptotic effects of ginsenoside Rb1 on FK506-induced apoptosis were mediated by the inhibition of mitogen-activated protein kinases and caspase activation.

Keywords

caspase-3; FK506-induced nephrotoxicity; ginsenoside Rb1; KIM-1; MAPKs;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)

Reference
Cited By KSCI

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