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http://dx.doi.org/10.1016/j.jgr.2017.08.009

Research article Black ginseng activates Akt signaling, thereby enhancing myoblast differentiation and myotube growth  

Lee, Soo-Yeon (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University)
Go, Ga-Yeon (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University)
Vuong, Tuan Anh (Department of Molecular Cell Biology, Single Cell Network Research Center, Sungkyunkwan University School of Medicine)
Kim, Jee Won (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University)
Lee, Sullim (Natural Products Research Institute, Korea Institute of Science and Technology)
Jo, Ayoung (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University)
An, Jun Min (Ginseng by Pharm Co., Ltd.)
Kim, Su-Nam (Natural Products Research Institute, Korea Institute of Science and Technology)
Seo, Dong-Wan (College of Pharmacy, Dankook University)
Kim, Jin-Seok (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University)
Kim, Yong Kee (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University)
Kang, Jong-Sun (Department of Molecular Cell Biology, Single Cell Network Research Center, Sungkyunkwan University School of Medicine)
Lee, Sang-Jin (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University)
Bae, Gyu-Un (Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University)
Publication Information
Journal of Ginseng Research / v.42, no.1, 2018 , pp. 116-121 More about this Journal
Abstract
Background: Black ginseng (BG) has greatly enhanced pharmacological activities relative to white or red ginseng. However, the effect and molecular mechanism of BG on muscle growth has not yet been examined. In this study, we investigated whether BG could regulate myoblast differentiation and myotube hypertrophy. Methods: BG-treated C2C12 myoblasts were differentiated, followed by immunoblotting for myogenic regulators, immunostaining for a muscle marker, myosin heavy chain or immunoprecipitation analysis for myogenic transcription factors. Results: BG treatment of C2C12 cells resulted in the activation of Akt, thereby enhancing hetero-dimerization of MyoD and E proteins, which in turn promoted muscle-specific gene expression and myoblast differentiation. BG-treated myoblasts formed larger multinucleated myotubes with increased diameter and thickness, accompanied by enhanced Akt/mTOR/p70S6K activation. Furthermore, the BG treatment of human rhabdomyosarcoma cells restored myogenic differentiation. Conclusion: BG enhances myoblast differentiation and myotube hypertrophy by activating Akt/mTOR/p70S6k axis. Thus, our study demonstrates that BG has promising potential to treat or prevent muscle loss related to aging or other pathological conditions, such as diabetes.
Keywords
Akt signaling; black ginseng; myoblast differentiation; myogenic conversion; myotube hypertrophy;
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Times Cited By KSCI : 1  (Citation Analysis)
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