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http://dx.doi.org/10.1016/j.jgr.2016.09.001

Applications of molecular markers in the discrimination of Panax species and Korean ginseng cultivars (Panax ginseng)  

Jo, Ick Hyun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Young Chang (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Dong Hwi (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Kee Hong (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Hyun, Tae Kyung (Department of Industrial Plant Science and Technology, Chungbuk National University)
Ryu, Hojin (Department of Biology, Chungbuk National University)
Bang, Kyong Hwan (Department of Planning and Coordination, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Publication Information
Journal of Ginseng Research / v.41, no.4, 2017 , pp. 444-449 More about this Journal
Abstract
The development of molecular markers is one of the most useful methods for molecular breeding and marker-based molecular associated selections. Even though there is less information on the reference genome, molecular markers are indispensable tools for determination of genetic variation and identification of species with high levels of accuracy and reproducibility. The demand for molecular approaches for marker-based breeding and genetic discriminations in Panax species has greatly increased in recent times and has been successfully applied for various purposes. However, owing to the existence of diverse molecular techniques and differences in their principles and applications, there should be careful consideration while selecting appropriate marker types. In this review, we outline the recent status of different molecular marker applications in ginseng research and industrial fields. In addition, we discuss the basic principles, requirements, and advantages and disadvantages of the most widely used molecular markers, including restriction fragment length polymorphism, random amplified polymorphic DNA, sequence tag sites, simple sequence repeats, and single nucleotide polymorphisms.
Keywords
high-resolution melting; Panax ginseng; molecular marker; single-nucleotide polymorphism;
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