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http://dx.doi.org/10.1016/j.jgr.2016.05.004

Identification of ginseng root using quantitative X-ray microtomography  

Ye, Linlin (Shanghai Institute of Applied Physics, Chinese Academy of Sciences)
Xue, Yanling (Shanghai Institute of Applied Physics, Chinese Academy of Sciences)
Wang, Yudan (Shanghai Institute of Applied Physics, Chinese Academy of Sciences)
Qi, Juncheng (School of Information and Communication Engineering, North University of China)
Xiao, Tiqiao (Shanghai Institute of Applied Physics, Chinese Academy of Sciences)
Publication Information
Journal of Ginseng Research / v.41, no.3, 2017 , pp. 290-297 More about this Journal
Abstract
Background: The use of X-ray phase-contrast microtomography for the investigation of Chinese medicinal materials is advantageous for its nondestructive, in situ, and three-dimensional quantitative imaging properties. Methods: The X-ray phase-contrast microtomography quantitative imaging method was used to investigate the microstructure of ginseng, and the phase-retrieval method is also employed to process the experimental data. Four different ginseng samples were collected and investigated; these were classified according to their species, production area, and sample growth pattern. Results: The quantitative internal characteristic microstructures of ginseng were extracted successfully. The size and position distributions of the calcium oxalate cluster crystals (COCCs), important secondary metabolites that accumulate in ginseng, are revealed by the three-dimensional quantitative imaging method. The volume and amount of the COCCs in different species of the ginseng are obtained by a quantitative analysis of the three-dimensional microstructures, which shows obvious difference among the four species of ginseng. Conclusion: This study is the first to provide evidence of the distribution characteristics of COCCs to identify four types of ginseng, with regard to species authentication and age identification, by X-ray phase-contrast microtomography quantitative imaging. This method is also expected to reveal important relationships between COCCs and the occurrence of the effective medicinal components of ginseng.
Keywords
Panax ginseng; Panax quinquefolius; quantitative microtomography; synchrotron radiation; X-ray phase contrast imaging;
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1 Wei X, Xiao TQ, Liu LX, Du GH, Chen M, Luo YY, Xu HJ. Application of x-ray phase contrast imaging to microscopic identification of Chinese medicines. Phys Med Biol 2005;50:4277-86.   DOI
2 Xie HL, Deng B, Du GH, Fu YN, Chen RC, Zhou GZ, Ren YQ, Wang YD, Xue YL, Peng GY, et al. Latest advances of X-ray imaging and biomedical application beamline at SSRF. Nucl Sci Tech 2015;26:020102-16.
3 Nugent KA, Gureyev TE, Cookson DF, Paganin D, Barnea Z. Quantitative phase imaging using hard X-rays. Phys Rev Lett 1996;77:2961-4.   DOI
4 Chen RC, Xie HL, Rigon L, Longo R, Castelli E, Xiao TQ. Phase retrieval in quantitative X-ray microtomography with a single sample-to-detector distance. Opt Lett 2011;36:1719-21.   DOI
5 Ye LL, Xue YL, Ni LH, Tan H, Wang YD, Xiao TQ. Application of X-ray phase contrast micro-tomography to the identification of traditional Chinese medicines. J Instrum 2013;8. http://dx.doi.org/10.1088/1748-0221/1088/1007/C07006.
6 Xiao TQ, Bergamaschi A, Dreossi D, Longo R, Olivo A, Pani S, Rigon L, Rokvic T, Venanzi C, Castelli E. Effect of spatial coherence on application of in-line phase contrast imaging to synchrotron radiation mammography. Nucl Instrum Meth A 2005;548:155-62.   DOI
7 Chen RC, Liu P, Xiao TQ, Xu LX. The X-ray Imaging for nondestructive microstructure analysis at SSRF. Adv Mater 2014;26:7688-91.   DOI
8 Ye LL, Xue YL, Tan H, Chen RC, Qi JC, Xiao TQ. X-ray phase contrast microtomography and its application in quantitative 3D imaging study of wild ginseng characteristic microstructures. Acta Opt Sin 2013;33:1234002-6.   DOI
9 Li WK, Gu CG, Zhang HJ, Awang DVC, Fitzloff JF, Fong HH, van Breemen RB. Use of high-performance liquid chromatography-tandem mass spectrometry to distinguish Panax ginseng C. A. Meyer (Asian Ginseng) and Panax quinquefolius L. (North American Ginseng). Anal Chem 2000;72:5417-22.   DOI
10 Liu JH, Burdette JE, Xu HY, Gu CG, van Breemen RB, Bhat KP, Booth N, Constantinou AI, Pezzuto JM, Fong HH, et al. Evaluation of estrogenic activity of plant extracts for the potential treatment of menopausal symptoms. J Agric Food Chem 2001;49:2472-9.   DOI
11 Reynolds LB. Effects of harvest date on some chemical and physical characteristics of American ginseng (Panax quinquefolius L.). J Herbs Spices Med Plants 1998;6:63-9.   DOI
12 Christensen LP. Ginsenosides chemistry, biosynthesis, analysis and potential health effects. Adv Food Nutr Res 2009;55:1-99.
13 Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pharmacol 1999;58:1685-93.   DOI
14 Fournier AR, Proctor JT, Gauthier L, Khanizadeh S, Belanger A, Gosselin A, Dorais M. Understory light and root ginsenosides in forest-grown Panax quinquefolius. Phytochemistry 2003;63:777-82.   DOI
15 Konsler TR, Zito SW, Shelton JE, Staba EJ. Lime and phosphorus effects on American ginseng: II. Root and leaf ginsenoside content and their relationship. J Am Soc Hort Sci 1990;115:575-80.
16 Jackson C, Dini JP, Lavandier C, Faulkner H, Rupasinghe HP, Proctor JT. Ginsenoside content of North American ginseng (Panax quinquefolius L. Araliaceae) in relation to plant development and growing locations. J Ginseng Res 2003;27:135-40.   DOI
17 Reynolds LB. Effects of drying on chemical and physical characteristics of American ginseng (Panax quinquefolius L.). J Herbs Spices Med Plants 1998;6:9-21.
18 Zhang YC, Li G, Jiang C, Yang B, Yang HJ, Xu HY, Huang LQ. Tissue-specific distribution of ginsenosides in different aged ginseng and antioxidant activity of ginseng leaf. Molecules 2014;19:17381-99.   DOI
19 Zhan DQ, Zhang XM, Sun SQ. Wavelet-transform based identification of ginseng of different ages using two-dimensional infrared correlation spectroscopy. Spectrosc Spect Anal 2007;27:1497-501.
20 Shi W, Wang YT, Li J, Zhang HQ, Ding L. Investigation of ginsenosides in different parts and ages of Panax ginseng. Food Chem 2007;102:664-8.   DOI
21 Kim N, Kim K, Choi BY, Lee DH, Shin YS, Bang KH, Cha SW, Lee JW, Choi HK, Jang DS, et al. Metabolomic approach for age discrimination of Panax ginseng using UPLC-Q-TOF MS. J Agric Food Chem 2011;59:10435-41.   DOI
22 Zhao ZZ. An illustrated microscopic identification of Chinese materia medica. Shenyang: Liaoning Science and Technology Publishing House; 2005.
23 Kang TG. Authentication of Chinese medicinal material monographs. Beijing: China Press of Traditional Chinese Medicine; 2007.
24 Wilkins SW, Gureyev TE, Gao D, Pogany A, Stevenson AW. Phase-contrast imaging using polychromatic hard X-rays. Nature 1996;384:335-8.   DOI
25 Clarke LP, Velthuizen RP, Camacho MA, Heine JJ, Vaidyanathan M, Hall LO, Thatcher RW, Silbiger ML. MRI segmentation: methods and applications. Magn Reson Imaging 1995;13:343-68.   DOI
26 Jung IC, Jeong IS, Kim CS. Distinction of internal tissue of raw ginseng root using a computed tomography scanner. J Ginseng Res 2012;36:469-76.   DOI
27 Yuan CS, Wang CZ, Wicks SM, Qi LW. Chemical and pharmacological studies of saponins with a focus on American ginseng. J Ginseng Res 2010;34:160-7.   DOI
28 Heeraman DA, Hopmans JW, Clausnitzer V. Three dimensional imaging of plant roots in situ with X-ray computed tomography. Plant Soil 1997;189:167-79.
29 Fitzgerald R. Phase-sensitive x-ray imaging. Phys Today 2000;53:23-6.
30 Smith SY, Smith, Collinson ME, Rudall PJ, Simpson DA, Marone F, Stampanoni M. Virtual taphonomy using synchrotron tomographic microscopy reveals cryptic features and internal structure of modern and fossil plants. PNAS 2009;106:12013-8.   DOI
31 Stuppy WH, Maisano JA, Colbert MW, Rudall PJ, Rowe TB. Three-dimensional analysis of plant structure using high-resolution X-ray computed tomography. Trends Plant Sci 2003;8:2-6.   DOI
32 Kim SA, Punshon T, Lanzirotti A, Li LT, Alonso JM, Ecker JR, Kaplan J, Guerinot ML. Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1. Science 2006;314:1295-8.   DOI
33 Xue YL, Xiao TQ, Du GH, Liu LX, Hu W, Xu HJ. Microscopic identification of Panax quinquefolius and Panax ginseng by X-ray phase contrast imaging. Acta Opt Sin 2008;28:1828-32.   DOI