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http://dx.doi.org/10.1016/j.jgr.2014.04.001

Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by ${\gamma}$-irradiation ($^{60}Co$) of adventitious roots  

Zhang, Jun-Ying (Faculty of Biotechnology, Jeju National University)
Sun, Hyeon-Jin (Subtropical Horticulture Research Institute, Jeju National University)
Song, In-Ja (Subtropical Horticulture Research Institute, Jeju National University)
Bae, Tae-Woong (Subtropical Horticulture Research Institute, Jeju National University)
Kang, Hong-Gyu (Subtropical Horticulture Research Institute, Jeju National University)
Ko, Suk-Min (Subtropical Horticulture Research Institute, Jeju National University)
Kwon, Yong-Ik (Subtropical Horticulture Research Institute, Jeju National University)
Kim, Il-Woung (Subtropical Horticulture Research Institute, Jeju National University)
Lee, Jaechun (School of Medicine, Jeju National University)
Park, Shin-Young (Department of Clinical Pathology, Cheju Halla University)
Lim, Pyung-Ok (Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST))
Kim, Yong Hwan (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET))
Lee, Hyo-Yeon (Faculty of Biotechnology, Jeju National University)
Publication Information
Journal of Ginseng Research / v.38, no.3, 2014 , pp. 220-225 More about this Journal
Abstract
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants.
Keywords
mutant adventitious roots; Panax ginseng Meyer; somatic embryo; somatic embryogenesis;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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