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http://dx.doi.org/10.1016/j.jgr.2013.10.002

Re-evaluation of physicochemical and NMR data of triol ginsenosides Re, Rf, Rg2, and 20-gluco-Rf from Panax ginseng roots  

Cho, Jin-Gyeong (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
In, Seo-Ji (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Jung, Ye-Jin (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Cha, Byeong-Ju (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Lee, Dae-Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Yong-Bum (Technology Services Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Yeom, Myeonghun (Amorepacific Corporation Research and Development Center)
Baek, Nam-In (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Publication Information
Journal of Ginseng Research / v.38, no.2, 2014 , pp. 116-122 More about this Journal
Abstract
Ginseng roots were extracted with aqueous methanol, and extracts were suspended in water and extracted successively with ethyl acetate and n-butanol. Column chromatography using the n-butanol fraction yielded four purified triol ginseng saponins: the ginsenosides Re, Rf, Rg2, and 20-gluco-Rf. The physicochemical, spectroscopic, and chromatographic characteristics of the ginsenosides were measured and compared with reports from the literature. For spectroscopic analysis, two-dimensional nuclear magnetic resonance (NMR) methods such as $^1H$-$^1H$ correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity were employed to identify exact peak assignments. Some peak assignments for previously published $^1H$-and $^{13}C$-NMR spectra were found to be inaccurate. This study reports the complete NMR assignment of 20-gluco-Rf for the first time.
Keywords
fast atom bombardment/MS; HPLC; NMR; Panax ginseng; triol ginsenoside;
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Times Cited By KSCI : 5  (Citation Analysis)
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