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http://dx.doi.org/10.5142/jgr.2012.36.1.107

Fungal Endophytes from Three Cultivars of Panax ginseng Meyer Cultivated in Korea  

Park, Sang-Un (Department of Crop Science, Chungnam National University)
Lim, Hyoun-Sub (Department of Applied Microbiology, Chungnam National University)
Park, Kee-Choon (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Park, Young-Hwan (School of Biotechnology, Yeungnam University)
Bae, Han-Hong (School of Biotechnology, Yeungnam University)
Publication Information
Journal of Ginseng Research / v.36, no.1, 2012 , pp. 107-113 More about this Journal
Abstract
In order to investigate the diversity of endophytes, fungal endophytes in Panax ginseng Meyer cultivated in Korea were isolated and identified using internal transcribed spacer (ITS) sequences of ribosomal DNA. Three cultivars of 3-year-old ginseng roots (Chunpoong, Yunpoong, and Gumpoong) were used to isolate fungal endophytes. Surface sterilized ginseng roots were placed on potato dextrose agar plates supplemented with ampicilin and streptomycin to inhibit bacterial growth. Overall, 38 fungal endophytes were isolated from 12 ginseng roots. According to the sequence analysis of the ITS1-5.8S-ITS2, 38 fungal isolates were classified into 4 different fungal species, which were Phoma radicina, Fusarium oxysporum, Setophoma terrestris and Ascomycota sp. 2-RNK. The most dominant fungal endophyte was P. radicina in 3 cultivars. The percentage of dominant endophytes of P. radicina was 65.8%. The percentage of colonization frequency of P. radicina was 80%, 52.9%, and 75% in Chunpoong, Yunpoong, and Gumpoong, respectively. The second most dominant fungal endophyte was F. oxysporum. The diversity of the fungal endophytes was low and no ginseng cultivar specificity among endophytes was detected in this study. The identified endophytes can be potential fungi for the production of bioactive compounds and control against ginseng pathogens.
Keywords
Panax ginseng; Fungal endophytes; Internal transcribed spacer (ITS) sequence;
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Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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