Identification of 'Chunpoong' among Panax ginseng Cultivars Using Real Time PCR and SNP Marker
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Sun, Hua
(Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Lee, Ok-Ran (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) Kim, Yu-Jin (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) Jeong, Seok-Kyu (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) In, Jun-Gyo (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) Kwon, Woo-Saeng (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) Kim, Se-Young (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) Yang, Deok-Chun (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) |
1 | Sugaya A, Yuzurihara M, Tsuda T, Yasuda K, Kajiwara K, Sugaya E. Proliferative effect of ginseng saponin on neurite extension of primary cultured neurons of the rat cerebral cortex. J Ethnopharmacol. 22: 173-181 (1998) DOI ScienceOn |
2 | Jo JS, Han YN, Oh HI, Park H, Sung HS, Park JI. Korean ginseng has a characteristic shape. In: Han YN, Oh HI, Park H, Sung HS, Park JI (ed), Understandiny of Korean ginseng, The Society for Korean Ginseng, Hanrimwon, Seoul, p 37- 38 (1995) |
3 | Cheung WY, Hubert N, Landry BS. A simple and rapid DNA microextraction method for plant, animal, and insect suitable for RAPD and other PCR analyses. PCR Methods Appl. 3: 69-70 (1993) DOI |
4 | Guidet F. A powerful new technique to quickly prepare hundreds of plant extracts for PCR and RAPD analyses. Nucleic Acids Res. 22: 1772-1773 (1994) DOI |
5 | Dilworth E, Frey JE. A rapid method for high throughput DNA extraction from plant material for PCR amplification. Plant Mol Biol Rep. 18: 61-64 (2000) DOI |
6 | Chunwongse J, Martin GB, Tanksley SD. Pre-germination genotypic screening using PCR amplification of half-seeds. Theor Appl Genet. 86: 694-698 (1993) |
7 | Dalla CL, Vaccari I, Mandolini M, Martinelli L. Elaboration of a Reliable Strategy Based on Real-Time PCR To Characterize Genetically Modified Plantlets and To Evaluate the Efficiency of a Marker Gene Removal in Grape (Vitis spp.). J Agric Food Chem. 57: 2668-2677 (2009) DOI ScienceOn |
8 | Wu SB, Wirthensohn MG, Hunt P, Gibson JP, Sedgley M. High resolution melting analysis of almond SNPs derived from ESTs. Theor Appl Genet. 118: 1-14 (2008) DOI |
9 | Palazon J, Cusido R M, Bonfil M, Mallol A, Moyamo E, Marales C, Pinol MT. Elicitation of different Panax ginseng transformed root phenotypes for an improvement ginsenoside production. Plant Physiol Biochem. 41: 1019-1025 (2003) DOI ScienceOn |
10 | Mader E, Lukas B, Novak JA. Strategy to setup codominant microsatellite analysis for high-resolution-melting-curve-analysis (HRM). BMC Genet. 9: 69 (2008) DOI ScienceOn |
11 | Zheng K, Subudhi PK, Domingo J, Maopantay G, Huang N. Rapid DNA isolation for marker assisted selection in rice breeding. Rice Genet Newslett. 12: 48 (1995) |
12 | Wang H, Qi M, Cutler AJ. A simple method of preparing plant samples for PCR. Nucleic Acids Res. 21: 4153-4154 (1993) DOI ScienceOn |
13 | Ikeda N, Bautista NS, Yamada T, Kamijima O, Ishii, T. Ultrasimple DNA extraction method for marker-assisted selection using microsatellite markers in rice. Plant Mol Biol Rep. 19: 27-32 (2001) DOI ScienceOn |
14 | Garritano S, Gemignani F, Voegele C, Nguyen-Dumont T, Le Calvez-Kelm F, De Silva D, Lesueur F, Landi S, Tavtigian SV. Determining the effectiveness of high resolution melting Analysis for SNP genotyping and mutation scanning at the TP53 locus. BMC Genet. 10: 5 (2009) DOI ScienceOn |
15 | Sun H, Kim MK, Pulla RK, Kim YJ, Yang DC. Isolation and expression analysis of a novel major latex-like protein (MLP151) gene from Panax ginseng. Mol Biol Report, Online published (2009) |
16 | van Tienderen PH, de Haan AA, van der Linden CG, Vosman B. Biodiversity assessment using markers for ecologically important traits. Trends Ecol Evol. 17: 577-582 (2002) DOI ScienceOn |
17 | Do N, Adams RP. A simple technique for removing plant polysaccharide contaminants from DNA. Biotechniques 10: 162-6 (1991) |
18 | Callaway AS, Abranches R, Scroggs J, Allen GC, Thompson WF. High-throughput transgene copy number estimation by competitive PCR. Plant Mol Biol Rep. 20: 265-277 (2002) DOI |
19 | Joshi MU, Pittman HK, Haisch CE, Verbanac KM. Real-time PCR to determine transgene copy number and to quantitate the biolocalization of adoptively transferred cells from EGFP-transgenic mice. Biotechniques 45: 247-258 (2008) DOI ScienceOn |
20 | Garces-Claver A, Fellman SM, Gil-Ortega R, Jahn M, Arnedo- Andres MS. Identification, validation and survey of a single nucleotide polymorphism (SNP) associated with pungency in Capsicum spp. Theor Appl Genet. 115: 907-916 (2007) DOI |
21 | Ohnishi Y, Tanaka T, Ozaki K, Yamada R, Suzuki H, Nakamura Y. A high-throughput SNP typing system for genomewide association studies. J Hum Genet. 46: 471-477 (2001) DOI |
22 | Shiokai S, Kitashiba H, Shirasawa K, Nagano K, Nishio T. Leaf-punch method to prepare a large number of PCR templates from plants for SNP analysis. Mol Breeding 23:329- 336 (2009) DOI |
23 | Banerjce U, Izquierdo JA. Antistress and antifatigue properties of Panax ginseng: Comparison with piracetan. Acta Physiol Lat Am. 32: 277-285 (1982) |
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