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http://dx.doi.org/10.5142/JGR.2010.34.1.047

Identification of 'Chunpoong' among Panax ginseng Cultivars Using Real Time PCR and SNP Marker  

Sun, Hua (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Lee, Ok-Ran (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Yu-Jin (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Jeong, Seok-Kyu (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
In, Jun-Gyo (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Kwon, Woo-Saeng (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Se-Young (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Yang, Deok-Chun (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Publication Information
Journal of Ginseng Research / v.34, no.1, 2010 , pp. 47-50 More about this Journal
Abstract
The common DNA extraction methods are indispensable for genotyping by molecular marker analysis. However, genotyping a large number of plants is painstaking. A modified 'NaOH-Tris' method used in this study reduces the extraction time while keeping the cost low and avoiding the use of hazardous chemicals. The endpoint analysis by realtime PCR tends to be fast and effective for the development of SNP markers linked to the 'Chunpoong' cultivar of Panax ginseng. The 'Chunpoong' marker was developed by a major latex-like protein gene sequence. From our results, we suggest that this method is successful in distinguishing 'Chunpoong' from a large number of ginseng cultivars.
Keywords
Panax ginseng; chunpoong; real-time PCR; SNP;
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