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http://dx.doi.org/10.5142/JGR.2009.33.4.324

Regulatory Effect of Ginsenosides Rh1 on Monocytic U937 Cell Adhesion  

Kim, Byung-Hun (School of Bioscience and Biotechnology, Kangwon National University)
Cho, Jae-Youl (School of Bioscience and Biotechnology, Kangwon National University)
Publication Information
Journal of Ginseng Research / v.33, no.4, 2009 , pp. 324-329 More about this Journal
Abstract
Cell-cell adhesion managed by various adhesion molecules is known to be one of pathophysiological phenomena found in numerous immunological diseases such as rheumatoid arthritis and allergic diseases. In this study, we examined the regulatory role of ginsenosides (G)- Rh1, reported to display anti-inflammatory and anti-allergic effects, on CD29-mediated cell adhesion. G-Rh1 significantly suppressed U937 cell-cell adhesion mediated by CD29 but not CD43. It also blocked U937 cell-fibronectin adhesion, mediated by activated CD29, up to 30%. In agreement, this compound also significantly decreased the surface level of CD29 but not CD43 as well as other costimulatory molecules such as CD69, CD80, and CD86. Therefore, these results suggest that G-Rh1 may have inhibitory function on CD29-mediated cell adhesion events, probably contributing to its anti-inflammatory and anti-allergic activities.
Keywords
ginsenoside Rh1; cell-cell adhesion; CD29; monocytic cell; U937 cell;
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1 Wang YZ, Chen J, Chu SF, Wang YS, Wang XY, Chen NH, Zhang JT. Improvement of memory in mice and increase of hippocampal excitability in rats by ginsenoside Rg1's metabolites ginsenoside Rh1 and protopanaxatriol. J Pharmacol Sci. 109: 504-510 (2009)   DOI   ScienceOn
2 Park EK, Choo MK, Han MJ, Kim DH. Ginsenoside Rh1 possesses antiallergic and anti-inflammatory activities. Int Arch Allergy Immunol. 133: 113-120 (2004)   DOI   ScienceOn
3 Cho JY, Fox DA, Horejsi V, Sagawa K, Skubitz KM, Katz DR, Chain B. The functional interactions between CD98, beta1-integrins, and CD147 in the induction of U937 homotypic aggregation. Blood 98: 374-382 (2001)   DOI   ScienceOn
4 Larrucea S, Gonzalez-Rubio C, Cambronero R, Ballou B, Bonay P, Lopez-Granados E, Bouvet P, Fontan G, Fresno M, Lopez-Trascasa M. Cellular adhesion mediated by factor J, a complement inhibitor. Evidence for nucleolin involvement. J Biol Chem. 273: 31718-31725 (1998)   DOI   ScienceOn
5 Cho JY, Katz DR, Chain BM. Staurosporine induces rapid homotypic intercellular adhesion of U937 cells via multiple kinase activation. Br J Pharmacol. 140: 269-276 (2003)   DOI   ScienceOn
6 Cho JY, Chain BM, Vives J, Horejsi V, Katz DR. Regulation of CD43-induced U937 homotypic aggregation. Exp Cell Res. 290: 155-167 (2003)   DOI   ScienceOn
7 Cho JY, Kim AR, Joo HG, Kim BH, Rhee MH, Yoo ES, Katz DR, Chain BM, Jung JH. Cynaropicrin, a sesquiterpene lactone, as a new strong regulator of CD29 and CD98 functions. Biochem Biophys Res Commun. 313: 954-961 (2004)   DOI   ScienceOn
8 Cordes N, Park CC. beta1 integrin as a molecular therapeutic target. Int J Radiat Biol. 83: 753-760 (2007)   DOI   ScienceOn
9 Mettouchi A, Meneguzzi G. Distinct roles of beta1 integrins during angiogenesis. Eur J Cell Biol. 85: 243-247 (2006)   DOI   ScienceOn
10 Stonehouse TJ, Woodhead VE, Herridge PS, Ashrafian H, George M, Chain BM, Katz DR. Molecular characterization of U937-dependent T-cell co-stimulation. Immunology 96: 35-47 (1999)   DOI   ScienceOn
11 Woods ML, Shimizu Y. Signaling networks regulating beta1 integrin-mediated adhesion of T lymphocytes to extracellular matrix. J Leukoc Biol. 69: 874-880 (2001)   ScienceOn
12 Yusuf-Makagiansar H, Anderson ME, Yakovleva TV, Murray JS, Siahaan TJ. Inhibition of LFA-1/ICAM-1 and VLA-4/ VCAM-1 as a therapeutic approach to inflammation and autoimmune diseases. Med Res Rev. 22: 146-167 (2002)   DOI   ScienceOn
13 Samukawa K, Suzuki Y, Ohkubo N, Aoto M, Sakanaka M, Mitsuda N. Protective effect of ginsenosides Rg(2) and Rh(1) on oxidation-induced impairment of erythrocyte membrane properties. Biorheology 45: 689-700 (2008)   ScienceOn
14 Shin YW, Bae EA, Kim SS, Lee YC, Lee BY, Kim DH. The effects of ginsenoside Re and its metabolite, ginsenoside Rh1, on 12-O-tetradecanoylphorbol 13-acetate- and oxazoloneinduced mouse dermatitis models. Planta Med. 72: 376-378 (2006)   DOI   ScienceOn
15 Matsumoto M, Shigeta A, Miyasaka M, Hirata T. CD43 plays both antiadhesive and proadhesive roles in neutrophil rolling in a context-dependent manner. J Immunol. 181: 3628-3635 (2008)   ScienceOn
16 Cho JY. Chloroquine, an anti-malarial agent, acts as a novel regulator of beta 1-integrin-mediated cell-cell adhesion. Biol Pharm Bull. 31: 726-730 (2008)   DOI   ScienceOn
17 Deane JA, Hickey MJ. Molecular mechanisms of leukocyte trafficking in T-cell-mediated skin inflammation: insights from intravital imaging. Expert Rev Mol Med. 11: e25 (2009)   DOI   ScienceOn
18 Murtaugh MP, Foss DL. Inflammatory cytokines and antigen presenting cell activation. Vet Immunol Immunopathol. 87: 109-121 (2002)   DOI   ScienceOn
19 Caulfield JJ, Fernandez MH, Sousa AR, Lane SJ, Lee TH, Hawrylowicz CM. Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte-macrophage colony-stimulating factor in the presence of glucocorticoids. Immunology 98: 104-110 (1999)   DOI
20 Van Waes C. Cell adhesion and regulatory molecules involved in tumor formation, hemostasis, and wound healing. Head Neck 17: 140-147 (1995)   DOI   ScienceOn
21 Yun TK, Lee YS, Lee YH, Kim SI, Yun HY. Anticarcinogenic effect of Panax ginseng C.A. Meyer and identification of active compounds. J Korean Med Sci. 16 Suppl: S6-18 (2001)
22 Choi KT. Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer. Acta Pharmacol Sin. 29: 1109-1118 (2008)   DOI   ScienceOn
23 Kim BH, Lee YG, Park TY, Kim HB, Rhee MH, Cho JY. Ginsenoside Rp1, a ginsenoside derivative, blocks lipopolysaccharide-induced interleukin-1beta production via suppression of the NF-kappaB pathway. Planta Med. 75: 321-326 (2009)   DOI   ScienceOn
24 Kim YS, Kim DS, Kim SI. Ginsenoside Rh2 and Rh3 induce differentiation of HL-60 cells into granulocytes: modulation of protein kinase C isoforms during differentiation by ginsenoside Rh2. Int J Biochem Cell Biol. 30: 327-338 (1998)   DOI   ScienceOn