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http://dx.doi.org/10.5142/JGR.2009.33.4.278

Enhancement of Murine NK cell Activity in vitro by Red Ginseng Acidic Polysaccharide  

Choi, Hye-Sook (School of Pharmacy, Sungkyunkwan University)
Sohn, Eun-Wha (Department of Herbal Medicine Resources, Institute of Bioscience and Biotechnology, Kangwon National University)
Rhee, Dong-Kwon (School of Pharmacy, Sungkyunkwan University)
Pyo, Suh-Kneung (School of Pharmacy, Sungkyunkwan University)
Publication Information
Journal of Ginseng Research / v.33, no.4, 2009 , pp. 278-282 More about this Journal
Abstract
The in-vitro immunomodulatory function of the activity of murine natural-killer (NK) cells induced by redginseng acidic polysaccharide (RGAP) was examined. RGAP induced the significant enhancement of NK cell activity against the Yac-1 tumor cells. The treatment of splenocytes cultured with RGAP for 16 h resulted in a significant increase in NK activity at the E:T ratio of 100:1, and in a 239 and 250% increase at 10 and $100{\mu}g$/ml, respectively. We also demonstrate that RGAP treatment increased the production of interferon (IFN)-$\gamma$ (17-125%) and tumor necrosis factor (TNF)-${\alpha}$ (15-100%), suggesting that the increase in NK cell cytotoxicity could be due to the enhancement of the NK cell production of both cytokines. In addition, RGAP had a stimulating effect on lymphocyte proliferation in the presence of mitogens. Overall, these results suggest that RGAP has an immunopotentiating effect on NK cells, which can support the development of clinical studies on RGAP.
Keywords
red ginseng; NK cell; cytotoxicity; cytokines;
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