Browse > Article
http://dx.doi.org/10.5142/JGR.2009.33.3.212

Identification of Korean Ginseng Cytochrome P450 gene and Its Characterization by Transformation System  

Shim, Ju-Sun (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Yu-Jin (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Jung, Seok-Kyu (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Kwon, Woo-Saeng (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Se-Young (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Yang, Deok-Chun (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Publication Information
Journal of Ginseng Research / v.33, no.3, 2009 , pp. 212-218 More about this Journal
Abstract
Triterpenoid saponins were synthesized in Panax ginseng C.A. Meyer via the isoprenoid pathway by cyclization of 2,3-oxidosqualene to give primarily oleanane (beta-amyrin) or dammarane triterpenoid skeletons. The triterpenoids are backbone and undergoes various modifications (oxidation, substitution and glycosylation), mediated by cytochrome P450 (CYP)-dependent monooxygenases, glycosyltransferase and other enzymes. This is likely to be due in part to the complexity of the molecules and the lack of pathway intermediates for biochemical studies. A cDNA clone encoding a putative CYP gene was isolated from flower bud of ginseng and transformed into the plant(Nicotiana tabacum cv. Xanthi) and confirmed by PCR analysis. The CYP gene (PgCYP) contained an open reading frame(ORF) encoding mature protein of 500 amino acids. The expression of PgCYP were investigated in transgenic tobacco by reverse transcriptase-polymerase chain reaction (RT-PCR).
Keywords
cytochrome P450; gene; ginseng; tobacco;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Horsch RB, Fry J, Hoffmann N, Neldemeyer J, Rogers SG, Fraley RT. Leaf disc transformation. In Plant Molecular Biology: Manual Geivin SB Ed, Kluwer Academic Publishers, The Netherlands A5: 1-9 (1978)
2 Murashige T, Skoog GA. Reverse medium for repid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473-497 (1962)   DOI
3 Kim TG, Kim YS, Kwon TH. Expression of AmA1 gene encoding storage protein of amaranthus in transgenic tobacco. Korean J Plant Tissue Culture 27: 169-173 (2000)   과학기술학회마을
4 Choi KH, Jeon JH, Kim HS, Joung YH, Cho SJ, Lim YP, Joung H. Development of herbicide-resistant transgenic potato. Korean J Plant Tissue Culture 23: 161-165 (1996)
5 Choi KH, Yang DC, Jeon JH, Kim HS, Joung YH, Joung H. Expression of cold-regulated gene in transgenic Solanum tuberosum L. Korean J Plant Tissue Culture 23: 311-315 (1996)
6 Durst F. Biochemistry and physiology of plant cytochrome P450. Microbial and plant cytochrome P450 : Biochemical characteristics genetic engineering and practical implications. Ruckpaul K. and H. Rein ed. Taylor & Francis, London. p. 191-233 (1991)
7 Porter TD, Coon MJ. Cytochrome P450 : Multiplicity of isoforms, substrates, and catalytic and regulatory mechanim. J Biol Chem. 266: 13469-13472 (1991)
8 Wislocki PG, Miwa GT, Lu AYH. Enzymatic basis of detoxification. Jakoby, WB ed Academic Press New York Vol 1 p.135-182 (1980)
9 Ando T, Tanaka O, Shibata S. Chemical studies on the oriental plant drugs (XXV). Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Syoyakugaku Zasshi 25: 28-32 (1971)   과학기술학회마을
10 Christen P, Roberts MF, Phillipson JD, Evans WC. High-yield production of tropane alkaloids by hairy-root cultures of a Datura candida hybrid. Plant Cell Reports 8: 75-77 (1989)   DOI   ScienceOn
11 Haralampidis K, Trojanowska M, Osbourn AE. Biosynthesis of triterpenoid saponins in plants. Adv Biochem Eng Biotechnol. 75: 31-49 (2002)   DOI   ScienceOn
12 Morris PC, Kumar A, Bowles D, Cuming A. Osmotic stress and abscisic acid regulate the expression of the Em gene of wheat. Eur J Biochem. 190: 625-630 (1990)   DOI   ScienceOn
13 Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 205: 03-410 (1990)   DOI
14 Odell JT, Nagy F, Chua NH. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313: 810-812 (1985)   DOI   ScienceOn
15 Datla RSS, Hammerlindl JK, Panchuk B, Pelcher LE, Keller W. Modified binary plant transformation vectors with the wild-type gene encoding NPTII. Gene 211: 383-384 (1992)   DOI   ScienceOn
16 Hoekema A, VanHaaren MJJ, Fellinger AJ, Hooykaas PJJ, Schilperoort RA. Non-oncogenic plant vectors for use in the Agrobacterium binary system. Plant Mol Biol. 5: 85-89 (1985)   DOI   ScienceOn
17 Ditta G, Stanfield S, Corbin D, Helinski DR. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77: 7347-7351 (1980)   DOI   ScienceOn
18 Jianyong W, Zhong JJ. Production of ginseng and its bioactive components in plant cell culture: current technological and applied aspects. J Biotechnol. 68: 89-99 (1999)   DOI   ScienceOn
19 Chung YS, Chang YH, Sung JH. The effect of ginseng and caffeine products on the antioxidative activities of mouse kidney. J Ginseng Res. 30: 15-21 (2006)   과학기술학회마을   DOI   ScienceOn