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http://dx.doi.org/10.5142/JGR.2009.33.1.026

The Protective Effect of Ginseng Saponin against High Glucose-Induced Secretion of Insulin-Like Growth Factor (IGF)-I in Primary Cultured Rabbit Proximal Tubule Cells  

Jung, Ho-Kyoung (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Lim, Suel-Ki (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Park, Min-Jung (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Bae, Chun-Sik (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Yoon, Kyung-Chul (Department of Ophthalmology, Chonnam National University Medical School and Hospital)
Han, Ho-Jae (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Park, Soo-Hyun (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Publication Information
Journal of Ginseng Research / v.33, no.1, 2009 , pp. 26-32 More about this Journal
Abstract
Diabetic nephropathy is associated with the dysfunction of proximal tubule cells. Insulin-like growth factor 1(IGF-I) has also been considered to play an important role in the development of diabetic nephropathy. Ginsenosides have been used as a remedy for diabetes in Asian countries. Therefore, we examined the preventive effect of ginsenosides against high glucose-induced alteration of IGF-I secretion in the primary cultured proximal tubule cells. In present study, Ginseng saponin (GS) completely blocked high glucose-induced stimulation of IGF-I secretion in proximal tubule cells, whereas panaxatriol (PI) and panaxadiol (PD) partially suppressed. In addition, high glucose stimulated cAMP formation and protein kinase C(PKC) activity from cytosolic to membrane fraction. GS completely prevented high glucose-induced stimulation of cAMP and PKC activity while PT and PD partially did. Furthermore, high glucose-induced stimulation of IGF-I was blocked by the treatment of PKI (protein kinase A inhibitor) and bisindolylmaleimide I (protein kinase C inhibitor). In conclusion, GS prevented high glucose-induced dysfunction of proximal tubule cells.
Keywords
Ginsenosides; diabetic nephropathy; proximal tubule cells; IGF-I; cAMP; protein kinase C;
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1 Wang, S. N., Lapage, J. and Hirschberg, R. : Glomerular ultrafiltration and apical tubular action of IGF-I, TGF-beta, and HGF in nephrotic syndrome. Kidney Int. 56(4), 1247-1251 (1999)   DOI   ScienceOn
2 Cummings, E. A., Sochett, E. B., Dekker, M. G., Lawson, M. L. and Daneman, D. : Contribution of growth hormone and IGF-I to early diabetic nephropathy in type 1 diabetes. Diabetes 47(8), 1341-1346 (1998)   DOI   ScienceOn
3 Samikkannu, T., Thomas, J. J., Bhat, G. J., Wittman, V. and Thekkumkara, T. J. : Acute effect of high glucose on longterm cell growth: a role for transient glucose increase in proximal tubule cell injury. Am. J. Physiol. Renal Physiol. 291(1), F162-F175 (2006)   DOI   ScienceOn
4 Park, S. H., Choi, H. J., Lee, J. H., Woo, C. H., Kim, J. H. and Han, H. J. : High glucose inhibits renal proximal tubule cell proliferation and involves PKC, oxidative stress, and TGF-beta. Kidney Int. 59(5), 1695-1705 (2001)   DOI   ScienceOn
5 Cho, W. C., Chung, W. S., Lee, S. K., Leung, A. W., Cheng, C. H. and Yue, K. K. : Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats. Eur. J. Pharmacol. 550(1-3), 173-179 (2006)   DOI   ScienceOn
6 Kang, K. S., Yamabe, N., Kim, H. Y., Park, J. H. and Yokozawa, T. : Therapeutic potential of 20(S)-ginsenoside Rg(3) against streptozotocin-induced diabetic renal damage in rats. Eur. J. Pharmacol. 591(1-3), 266-272 (2008)   DOI   ScienceOn
7 Klar, J., Sandner, P., Muller, M. W. and Kurtz, A. : Cyclic AMP stimulates renin gene transcription in juxtaglomerular cells. Pflugers Arch. 444(3), 335-344 (2002)   DOI   ScienceOn
8 Locatelli, F., Pozzoni, P. and Del Vecchio, L. : Renal replacement therapy in patients with diabetes and end-stage renal disease. J. Am. Soc. Nephrol. 15, S25-29 (2004)   DOI   ScienceOn
9 Thomson, S. C., Vallon, V. and Blantz, R. C. : Kidney function in early diabetes: the tubular hypothesis of glomerular filtration. Am. J. Physiol. Renal Physiol. 286, F8-15 (2004)   DOI   ScienceOn
10 Yung, S., Lee, C. Y., Zhang, Q., Lau, S. K., Tsang, R. C. and Chan, T. M. : Elevated glucose induction of thrombospondin-1 up-regulates fibronectin synthesis in proximal renal tubular epithelial cells through TGF-beta1 dependent and TGF-beta1 independent pathways. Nephrol. Dial. Transplant. 21, 1504-1513 (2006)   DOI   ScienceOn
11 Iori, E., Marescotti, M. C., Vedovato, M., Ceolotto, G., Avogaro, A., Tiengo, A., Del Prato, S. and Trevisan, R. : In situ protein Kinase C activity is increased in cultured fibroblasts from Type 1 diabetic patients with nephropathy. Diabetologia. 46(4), 524-530 (2003)   DOI   ScienceOn
12 Kim, H. Y., Kang, K. S., Yamabe, N., Nagai, R. and Yokozawa, T. : Protective effect of heat-processed American ginseng against diabetic renal damage in rats. J. Agric. Food Chem. 55, 8491-8497 (2007)   DOI   ScienceOn
13 Jackson, E. K. and Dubey, R. K. : Role of the extracellular cAMP-adenosine pathway in renal physiology. Am. J. Physiol. Renal Physiol. 281(4), F597-612 (2001)   DOI
14 Kimura, M., Ishizawa, M., Miura, A., Itaya, S., Kanoh, Y., Yasuda, K., Uno, Y., Morita, H. and Ishizuka, T. : Platelet protein kinase C isoform content in type 2 diabetes complicated with retinopathy and nephropathy. Platelets 12(3), 138-143 (2001)   DOI   ScienceOn
15 Chung, S. D., Alavi, N., Livingston, D., Hiller, S. and Taub, M. : Characterization of primary rabbit kidney cultures that express proximal tubule functions in a hormonally defined medium. J. Cell Biol. 95(1), 118-126 (1982)   DOI   ScienceOn
16 Daughaday, W. H. and Rotwein, P. : Insulin-like growth factors I and II. Peptide messenger ribonucleic acid and gene structure serum, and tissue concentrations. Endocr. Rev. 10, 68-91 (1989)   DOI   ScienceOn
17 Bowsher, R. R., Lee, W. H., Apathy, J., O'Brien, P. J., Ferguson, A. L. and Henry, D. P. : Measurement of insulin-like growth factor-II in physiological fluids and tissues. I. An improved extraction procedure and radioimmunoassay for human and rat fluids. Endocrinology 128, 805-814 (1991)   DOI   ScienceOn
18 Inada, A., Kanamori, H., Arai, H., Akashi, T., Araki, M., Weir, G. C. and Fukatsu, A. : A model for diabetic nephropathy: advantages of the inducible cAMP early repressor transgenic mouse over the streptozotocin-induced diabetic mouse. J. Cell Physiol. 215(2), 383-391 (2008)   DOI   ScienceOn
19 Akturk, M., Arslan, M., Altinova, A., Ozdemir, A., Ersoy, R., Yetkin, I., Ayvali, E., Gonen, S. and Toruner, F. : Association of serum levels of IGF-I and IGFBP-1 with renal function in patients with type 2 diabetes mellitus. Growth Horm. IGF. Res. 17, 186-193 (2007)   DOI   ScienceOn
20 Park, K. S., Lee, D. E., Sung, J. H. and Chung, S. H. : Comparisons of antidiabetic effect of panax ginseng on MLD STZ-induced diabetic rats in terms of time of administration. J. Ginseng Res. 26(4), 191-195 (2002)   과학기술학회마을   DOI   ScienceOn
21 Wang, J., Huang, H., Liu, P., Tang, F., Qin, J., Huang, W., Chen, F., Guo, F., Liu, W. and Yang, B. : Inhibition of phosphorylation of p38 MAPK involved in the protection of nephropathy by emodin in diabetic rats. Eur. J. Pharmacol. 553(1-3), 297-303 (2006)   DOI   ScienceOn
22 Park, S. H., Choi, H. J., Lee, J. H., Yang, I. S. and Han, H. J. : High glucose stimulates Ca2+ uptake via cAMP and PLC/PKC pathways in primary cultured renal proximal tubule cells. Kidney Blood Press. Res. 24(1), 10-17 (2001)   DOI   ScienceOn
23 Koya, D. and King, G. L. : Protein kinase C activation and the development of diabetic complications. Diabetes 47(6), 859-866 (1998)   DOI   ScienceOn
24 Whiteside, C. I. and Dlugosz, J. A. : Mesangial cell protein kinase C isozyme activation in the diabetic milieu. Am. J. Physiol. Renal. Physiol. 282(6), F975-980 (2002)   DOI   ScienceOn
25 Francki, A., Motamed, K., McClure, T. D., Kaya, M., Murri, C., Blake, D. J., Carbon, J. G. and Sage, E. H. : SPARC regulates cell cycle progression in mesangial cells via its inhibition of IGF-dependent signaling. J. Cell Biochem. 88(4), 802-811 (2003)   DOI   ScienceOn
26 Yip, T. T., Lau, C. N., Kong, Y. C., Yung, K. H., Kim, J. H. and Woo, W. S. : Ginsenoside compositions of Panax ginseng C.A. Meyer tissue culture and juice. Am. J. Chin. Med. 13, 89-92 (1985)   DOI   ScienceOn
27 Sandra, A., Boes, M., Dake, B. L., Stokes, J. B. and Bar, R. S. : Infused IGF-I/IGFBP-3 complex causes glomerular localization of IGF-I in the rat kidney. Am. J. Physiol. 275, E32-E37 (1998)   ScienceOn
28 Tack, I., Elliot, S. J., Potier, M., Rivera, A., Striker, G. E. and Striker, L. J. : Autocrine activation of the IGF-I signaling pathway in mesangial cells isolated from diabetic NOD mice. Diabetes 51, 182-188 (2002)   DOI   ScienceOn
29 Kiefer, D. and Pantuso, T. : Panax ginseng. Am. Fam. Physician 68, 1539-1542 (2003)   ScienceOn
30 Waki, I., Kyo, H., Yasuda, M. and Kimura, M. : Effects of a hypoglycemic component of ginseng radix on insulin biosynthesis in normal and diabetic animals. J. Pharmacobiodyn. 5(8), 547-554 (1982)   DOI
31 Xie, J. T., Mehendale, S. R., Wang, A., Han, A. H., Wu, J. A., Osinski, J. and Yuan, C. S. : American ginseng leaf: ginsenoside analysis and hypoglycemic activity. Pharmacol. Res. 49(2), 113-117 (2004)   DOI   ScienceOn
32 Cho, W. C., Chung, W. S., Lee, S. K., Leung, A. W., Cheng, C. H. and Yue, K. K. : Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats. Eur. J. Pharmacol. 550(1-3), 173-179 (2006)   DOI   ScienceOn
33 Park, S. H., Shin, S. S. and Han, H. J. : High glucose levels alter angiotensin II induced Ca2+ uptake via PKC and cAMP pathways in renal proximal tubular cells. Kidney Blood Press. Res. 24(2), 84-91 (2001)   DOI   ScienceOn