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http://dx.doi.org/10.5142/JGR.2008.32.3.270

Inhibition Effect of Ginseng Saponin on the Growth of Citrobacterer sp. Isolated from Contaminated Ginseng  

Park, Chae-Kyu (KT&G Central Research Institute)
Kwak, Yi-Seong (KT&G Central Research Institute)
Hong, Soon-Gi (KT&G Central Research Institute)
Lee, Hoon-Sang (KT&G Central Research Institute)
Hwang, Mi-Sun (KT&G Central Research Institute)
Rhee, Man-Hee (Department of Veterinary Physiology, Kyungpook National University)
Won, Jun-Yeon (Joongbu University)
Han, Gyeong-Ho (KT&G Central Research Institute)
Publication Information
Journal of Ginseng Research / v.32, no.3, 2008 , pp. 270-274 More about this Journal
Abstract
A bacterium isolated from contaminated white ginseng was identified using API kit and electron microscope. This isolate was determined as rod shaped bacterium having about 1.0 ${\mu}m$ in diameter and 2.0 to 6.0 ${\mu}m$ in length. It had motility by peritrichous flagellum. The isolate had ${\beta}-galactosidase$, arginine dihydrolase and ornithin decarboxylase. It did not have ability not only to use citrate as sole carbon source and but also to produce $H_2S$. However, it could ferment glucose, manitol, sorbitol, rhamnose, arabinose and amygdalin. From these obserbations, the isolate was identified as Citrobacter sp. Ginseng saponin was added to culture of Citrobacter sp. in order to investigate saponin's influence on its growth. The strain was incubated at $38^{\circ}C$ for 3 days after addition of 0.05, 0.5, 2.0 and 4.0% (w/v) of saponin, respectively and the growth rates was investigated. The relative bacterial growth inhibition rates showed 28.6, 66.7, 92.4 and 97.7%, respectively, when compared with saponin non-treated group. These results suggest that the growth of Citrobacter sp. is inhibited by saponin in a concentration-dependent manner.
Keywords
ginseng; saponin; Citrobacter sp.; growth inhibition;
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Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Kwak, Y. S. and Chang, J. K. : Effect of various sterilization methods on growth of microorganism contaminated in ginseng powder. J. Fd Hyg. Safety. 16(3), 221-226 (2001)   과학기술학회마을
2 남성희 : 인삼성분이 초산발효에 미치는 영향에 관한 연구. 고려대학교 석사학위논문. pp. 27-30 (1979)
3 조대휘, 오승환, 유연현, 유태종 : Fusarium solani 와 Phytophthora cactorum이 고려인삼의 사포닌 성분변화에 미치는 영향. J. Ginseng Research 10(1), 180-189 (1986)
4 Kim, S. K., Kwak, Y. S., Kim, S. W., Hwang, S. Y., Ko, Y. S. and Yoo, C. M. : Improved methods for the preparation of crude ginseng saponin. J. Ginseng Research 22(3), 155-160 (1998)   과학기술학회마을
5 Kwak, Y. S., No, K. B., Chang, J. K. and Choi, K. J. : Effect of ozone treatment on growth of microorganism contaminated ginseng powders. J. Fd Hyg. Safety. 10, 45-51 (1995)
6 Kwak, Y. S., Chang, J. K. and Choo, J. J. : Effect of alcohol treatment on growth of microorganism contaminated in ginseng powders. J. Fd Hyg. Safety. 12(3), 205-209 (1997)   과학기술학회마을
7 Noel, R. K. and John G. H. : Bergy's Manual of Systematic Bacteriology. Williams & Wilkins, Baltimore, USA (1984)
8 식품의약품안전청 : 식품공전. pp.399-400 (2000)
9 이상윤 : 식품산업에서 생약자원의 활용과 전망. 식품산업과 영양. 5(3), 21-26 (2000)
10 이진만, 이상한, 김환묵 : 약용식품으로 한약재의 이용. 식품산업과 영양, 5(1), 50-56 (2000)
11 Beach, F. W. and Davenport, R. R. : Methods in Microbiology. Vol. 4, pp. 153, Academic Press London and New York (1971)
12 The International Commision on Microbiological Specification for Foods : Factors affecting life and death of microorganisms. Microbial Ecology of Foods. Vol. 1, pp. 98, Academic Press, London, United Kingdom (1980)
13 Kwak, Y. S., Lee, J. T. and Y대, W. H. : An identification of Enterobacter sp. isolated from contaminated ginseng and inhibition effect of ginseng saponin on its growth.. J. Fd Hyg. Safety. 17(1), 26-30 (2002)
14 대한약학회 대한약품공업협회 : GMP 실시에 관련된 의약품의 안정성과 유효 기간 설정에 대하여. 제약공업을 위한 세미나, 서울, pp. 81-83 (1981)