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http://dx.doi.org/10.5142/JGR.2008.32.2.150

Scavenging Strategy of Panax ginseng Against Formed Free Radicals Under Stress of Mercuric Chloride in Rattus norvegicus  

Mahour, Kanhiya (Toxicology Laboratory, Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University)
Saxena, Prabhu N. (Toxicology Laboratory, Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University)
Publication Information
Journal of Ginseng Research / v.32, no.2, 2008 , pp. 150-154 More about this Journal
Abstract
Twenty five albino rats were divided into five groups for conducting this experiment. The first group was for positive control (Vitamin C, ascorbic acid), the second group was of Panax ginseng (10 mg/kg body weight) treated group after bio-activity assay, the third group was of mercuric chloride treated group (0.033 mg/kg body weight) based on calculating $LD_{50}$ 9.26 mg/kg body weight by probit analysis, the fourth group was of mercuric chloride (0.033 mg/kg body weight) followed by Panax ginseng (10 mg/kg body weight) and the fifth group was Panax ginseng (10 mg/kg body weight) followed by mercuric chloride (0.033 mg/kg body weight) treated group. The interval between intake of Panax ginseng and mercuric chloride was of 2 hours in groups, fourth and fifth respectively. Comparative free radical scavenging property of Panax ginseng was studied under three in vitro models (role model for calculating scavenging activity) viz. DPPH method (hydroxyl free radicals), Nitric oxide method (nitrile free radicals) and Lipid peroxidation (mercury free radicals).
Keywords
Ascorbic acid; 1,1 diphenyl 2-picryl hydrizide; thiobarbituric acid reactive substances; lipid peroxidation;
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1 Green, L. C., Wagner, D. A., Glogowski, J., Skipper, P. L., Wishnok, J. S. and Tanncubaum, S. R. : Analysis of nitrate and $N^{15}$ in biological fluids. Anal Biochem. 126, 131 (1982)   DOI   ScienceOn
2 Marcocci, L., Maguire J. J., Droy-Lefaix, M. T. and Packer, L. : The nitric oxide scavenging property of Ginko biloba extracts EBG 761. Biochem Biophys Res Commun. 201, 748 (1994)   DOI   ScienceOn
3 Brraugghler, J. M., Duncan, C. A. and Chase L. R. : The involvement of iron in lipid peroxidaiton. Importance of ferrous and ferric ratio in initiation. J Biol Chem. 261, 10282 (1986)
4 Gutteridge, J. M. C. : Age pigments and free radicals: fluorescent lipid complexes formed by iron and copper containing proteins. Biochem Biophys Acta. 834, 144 (1985)   DOI   ScienceOn
5 Pryor, W. A. : Oxy-radicals and related species, their formation, lifetimes and reactions. Ann Rev Physiol. 48, 657 (1986)   DOI   ScienceOn
6 Ialenti, A., Moncada, S. and Di Rosa, M. : Modulation of adjuvant arthritis by endogenous nitric oxide. Br J Pharmacol. 110, 701 (1993)   DOI   ScienceOn
7 Sanchez-Moreno, C. : Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Tech Int. 8, 122-127 (2002)
8 Blos, M. S. : Antioxidant determinations by the use of stable free radical. Nature 26, 1199-1220 (1958)
9 Ross, R. : The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 362, 801-811 (1993)   DOI   ScienceOn
10 Shirwaikar, A. and Somashekar, A. P. : Anti-inflammatory activity and free radical scavenging studies of Aristolochia bracteolate Lam. Ind J Pharm. Sci. 65, 68 (2003)
11 Ohkawa, H., Ohishi, N. and Yagi, K. : Assay for lipid peroxides in animal tissues by thio barbituric acid reaction. Anal Biochem. 95, 351(1979)   DOI   ScienceOn
12 Kumar, P. V., Shashidhara, S., Kumar, M. M. and Sridhara, B. Y. : Effect of Luffa echinata on lipid peroxidation and free radical scavenging activity. J Pharm Pharmacol. 52, 891 (2000)   DOI   ScienceOn
13 Tiwari, A. K. : Imbalance in antioxidant defense and human diseases: multiple approach of natural antioxidants therapy. Curr Sci. 81, 1179-1187 (2001)
14 Saxena, P. N., Mahour, K. and Kumar, A. : Protective effect of Panax ginseng extract on renal functions altered by mercuric chloride in albino rats. J Ginseng Res. 30, 100-105 (2006)   과학기술학회마을   DOI   ScienceOn
15 Sreejayan, N. and Rao, M. N. A. : Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol. 49, 105 (1997)   DOI   ScienceOn
16 Halliwell, B. : Super oxide-dependent formation of hydroxyl free radicals in the presence of iron chelates. FEBS Lett. 92, 321 (1978)   DOI   ScienceOn
17 Gasoo, S., Sunol, C., Sanfeliu, C., Rodriguez-Farre, E. and Cristofol, R. M. : Pharmacological characterization of the effects of methyl mercury and mercuric chloride a spontaneous noradrenaline release from rat hippocampus slices. Life Sci. 67, 1210-1231(2000)
18 Finney, D. J. : Probit analysis. Cambridge University press, pp. 303 (1971)
19 Sreejayan, N. and Rao, M. N. A. : Free radical scavenging activity of curcuminoids. Drug Res. 46, 169 (1996)
20 Marcocci, L., Packer, L., Droy-Lefaiz, M. T., Sekaki, A. and Gardes-Albert, M. : Antioxidant action of Ginko biloba extracts Egb761. Meth Enzymol. 234, 462-467 (1994)   DOI