Ginsenoside Rb2 Upregulates the Low Density Lipoprotein Receptor Gene Expression through the Activation of the Sterol Regulated Element Binding Protein Maturation in HepG2 Cells |
Lim, Grewo
(Department of Biochemistry, College of Medicine, Konkuk University)
Lee, Hyunil (Department of Biochemistry, College of Medicine, Konkuk University) Kim, Eun-Ju (Department of Biochemistry, College of Medicine, Konkuk University) Noh, Yun-Hee (Department of Biochemistry, College of Medicine, Konkuk University) Ro, Youngtae (Department of Biochemistry, College of Medicine, Konkuk University) Koo, Ja-Hyun (Department of Biochemistry, College of Medicine, Konkuk University) |
1 | Kang, S. and Davis, R.A. : Cholesterol and hepatic lipoprotein assembly and secretion. Biochim Biophys Acta. 1529, 223-230 (2000) DOI ScienceOn |
2 | Rajendran, S. and Deepalakshmi, P.D. : Parasakthy K, Devaraj H, Devaraj SN. Effect of tincture of Crataegus on the LDLreceptor activity of hepatic plasma membrane of rats fed an atherogenic diet. Atherosclerosis. 123,235-241 (1996) DOI ScienceOn |
3 | Hua, X., Yokoyama, C., Wu, J., Briggs, M.R., Brown, MS., Goldstein, J.L. and Wang, X. : SREBP-2, a second basichelix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc Natl Acad Sci USA. 90,11603-11607 (1993) DOI ScienceOn |
4 | Gommori, K., Miyamoto, F., Shibata, Y., Higashi, T. and Sanada, S. : Effect of ginseng saponins on cholesterol metabolism. II. Effect of ginsenosides on cholesterol synthesis by liver slice. Chem Pharm Bull (Tokyo). 24, 2985-2987 (1976) DOI ScienceOn |
5 | Ikehara, M., Shibata, Y, Higashi, T., Sanada, S. and Shoji, J. : Effect of ginseng saponins on cholesterol metabolism. III. Effect of ginsenoside-Rb on cholesterol synthesis in rats fed on high-fat diet. Chem Pharm Bull (Tokyo). 26, 2844-2854 (1978) DOI ScienceOn |
6 | Sakakibara, K., Shibata, Y, Higashi, T., Sanada, S. and Shoji, J. : Effect of ginseng saponins on cholesterol metabolism. I. The level and the synthesis of serum and liver cholesterol in rats treated with ginsenosides. Chem Pharm Bull (Tokyo). 23, 1009-1016 (1975) DOI ScienceOn |
7 | Sato, R. and Takano, T. : Regulation of intracellular choles- terol metabolism. Cell Struct Funct. 20, 421-427 (1995) DOI ScienceOn |
8 | Field, F.J., Born, E., Murthy, S. and Mathur, S.N. Gene expression of sterol regulatory element-binding proteins in hamster small intestine. J Lipid Res. 42, 1-8 (2001) |
9 | Dujovne, C.A. and Harris, W.S. : The pharmacological treatment of dyslipidemia. Annu Rev Pharmacol Toxicol. 29, 265-288 (1989) DOI ScienceOn |
10 | Briggs, M.R., Yokoyama, C., Wang, X. and Brown, M.S. : Goldstein JL. Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. I. Identification of the protein and delineation of its target nucleotide sequence. J Biol Chem. 268, 14490-14496 (1993) |
11 | Smith, J.R., Osborne, T.E, Goldstein, J.L. and Brown, M.S. Identification of nucleotides responsible for enhancer activity of sterol regulatory element in low density lipoprotein receptor gene, J Biol Chem. 265,2306-2310 (1990) |
12 | Yokoyama, C., Wang, X., Briggs, M.R., Admon, A., Wu, J., Hua, X., Goldstein, J.L. and Brown, M.S.: SREBP-I, a basic-helix-loop-helix-leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell. 75,187-197 (1993) DOI |
13 | Goldstein, J.L. : Brown MS. Regulation of the mevalonate pathway. Nature. 343, 425-430 (1990) DOI ScienceOn |
14 | Yun, T.K. and Choi, SY Preventive effect of ginseng intake against various human cancers: a case-control study on 1987 pairs. Cancer Epidemiol Biomarkers Prev. 4, 401-408 (1995) |
15 | Yun, T.K. and Choi, S.Y. : Non-organ specific cancer prevention of ginseng: a prospective study in Korea. Int J Epidemiol. 27, 359-364 (1998) DOI ScienceOn |
16 | Wakabayashi, C., Hasegawa, H., Murata, J. and Saiki, I. : In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oncol Res. 9,411-417 (1997) |
17 | Nimpf, J. and Schneider, W.J. : From cholesterol transport to signal transduction: low density lipoprotein receptor, very low density lipoprotein receptor, and apolipoprotein E receptor-2. Biochim Biophys Acta. 1529, 287-298 (2000) DOI |
18 | Kang, S.Y., Kim, S.H., Schini, V.B. and Kim, N.D. : Dietary ginsenosides improve endothelium-dependent relaxation in the thoracic aorta of hypercholesterolemic rabbit. Gen Pharmacol. 26,483-487 (1995) DOI ScienceOn |
19 | Yokozawa, T., Kobayashi, T., Oura, H. and Kawashima, Y. : Hyperlipemia-improving effects of ginsenoside-Rb2 in strepto-zotocin-diabetic rats. Chem Pharm Bull (Tokyo). 33, 3893-3898 (1985) DOI ScienceOn |
20 | Sakai, L, Nohturfft, A., Goldstein, J.L., Brown, M.S. : Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies. J Biol Chem. 273, 5785-5793 (1998) DOI ScienceOn |
21 | Lim, G., Lee, H.I., Kim, E.J., Ro, Y.T., Noh, Y.H. and Koo, J.H. : The mechanism of LDL receptor up-regulation by ginsenoside Rb2 in HepG2 cultured under enriched cholesterol condition. J. Ginseng Res. 28(2), 87-93 (2004), (in Korean) DOI ScienceOn |
22 | Chomczynski, P. and Sacchi, N.: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem. 162, 156-159 (1987) |
23 | Hua, X., Sakai, J., Brown, M.S. and Goldstein, J.L. : Regulated cleavage of sterol regulatory element binding proteins requires sequences on both sides of the endoplasmic reticulum membrane. J Biol Chem. 271, 10379-10384 (1996) DOI |
24 | Harwood, H.J. Jr, Chandler, C.E., Pellarin, L.D., Bangerter, F.W., Wilkins, R.W, Long, C.A, Cosgrove, P.G, Malinow, M.R., Marzetta, C.A., Pettini, J.L., Savoy, Y.E. and Mayne, J.T. : Pharmacologic consequences of cholesterol absorption inhibition: alteration in cholesterol metabolism and reduction in plasma cholesterol concentration induced by the synthetic saponin beta-tigogenin cellobioside (CP-88818; tiqueside). J Lipid Res. 34, 377-395 (1993) |
25 | Sakai, J., Nohturfft, A., Cheng, D., Ho, Y.K., Brown, M.S., Goldstein, J.L.: Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SREBPs) and SREBP cleavage-activating protein. J Biol Chem. 272, 20213-20221 (1997) DOI ScienceOn |
26 | Morehouse, L.A., Bangerter, F.W., DeNinno, M.P., Inskeep, P.B., McCarthy, P.A., Pettini, J.L., Savoy, YE., Sugarman, E.D., Wilkins, R.W., Wilson, T.C., Woody, H.A., Zaccaro, L.M. and Chandler, C.E. : Comparison of synthetic saponin cholesterol absorption inhibitors in rabbits: evidence for a non-stoichiometric, intestinal mechanism of action. J Lipid Res. 40, 464-474 (1999) |
27 | Bursill, C., Roach, P.D., Bottema, C.D. and Pal, S. : Green tea upregulates the low-density lipoprotein receptor through the sterol-regulated element binding Protein in HepG2 liver cells. J Agric Food Chem. 49, 5639-5645 (2001) DOI ScienceOn |
28 | Yu-Poth, S., Yin, D., Zhao, G, Kris-Etherton, P.M., Etherton, T.D. : Conjugated linoleic acid upregulates LDL receptor gene expression in HepG2 cells. J Nutr. 134, 68-71 (2004) |
29 | Espenshade, P.J, Cheng, D., Goldstein, J.L., Brown, M.S. : Autocatalytic processing of site-l protease removes propeptide and permits cleavage of sterol regulatory element-binding proteins. J Biol Chem. 274, 22795-22804 (1999) DOI |
30 | Nagoshi, E., Imamoto, N., Sato, R. and Yoneda, Y. : Nuclear import of sterol regulatory element-binding protein-2, a basic helix-loop-helix-leucine zipper (bHLH-Zip)-containing transcription factor, occurs through the direct interaction of importin beta with HLH-Zip. Mol Biol Cell. 10, 2221-2233 (1999) DOI |
31 | Brown, M.S., Kovanen, P.T. and Goldstein, J.L. : Regulation of plasma cholesterol by lipoprotein receptors. Science. 212, 628-635 (1981) DOI |
32 | Koo, J.H., Noh, Y.H. and Lim, G. : Studies on the mechanism of the preventive effects of Korean ginseng components on hypercholesterolemia. The effects on the cholesterol content, the LDL-receptor and the enzymes related to cholesterol metabolism in Hep G2 cells cultured in cholesterol rich medium. Proceedings of '97 Korea-Japan Ginseng Symposium. pp.91-107 (1997), (in Korean) |
33 | Havekes, L.M, de Wit, E.C. and Princen, H.M. Cellular free cholesterol in Hep G2 cells is only partially available for down-regulation of low-density-lipoprotein receptor activity. Biochem J. 247, 739-746 (1987) DOI |
34 | Shimano, H.: Sterol regulatory element-binding proteins (SREBPs): transcriptional regulators of lipid synthetic genes. Prog Lipid Res. 40, 439-452 (2001) DOI ScienceOn |
35 | Brown, M.S. and Goldstein, J.L.: The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell. 89, 331-340 (1997) DOI ScienceOn |
36 | Joo, C.N. : Role of ginsenosides as bioreactor in lipid metabolism. J Nutr Sci Vitaminol (Tokyo). 298-301 (1992) |