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http://dx.doi.org/10.5142/JGR.2003.27.2.078

Production of Ginsenoside in Callus of Ginseng Hairy Roots  

Kwon, Jung-Hee (School of Life Sciences, College of Natural Sciences, Chungbuk National University, Institute for Basic Sciences)
Cheon, Hyun-Choon (School of Life Sciences, College of Natural Sciences, Chungbuk National University, Institute for Basic Sciences)
Yang, Deok-Cho (School of Life Sciences, College of Natural Sciences, Chungbuk National University, Institute for Basic Sciences)
Publication Information
Journal of Ginseng Research / v.27, no.2, 2003 , pp. 78-85 More about this Journal
Abstract
By the Agrobacterium rhizogenes A$_4$ were induced a transformed callus of ginseng hairy root and examine to find the possibility whether it can produce certain ginsenoside. Investigations for a finding out to optimal culture medium showed that BA application is better than more factorial composition between auxins and cytokinins. For the induction of hairy root callus of ginseng, l/2 MS medium containing 1 to 3 mg of benzyladenine(BA) per liter gave the best result. The growth of ginseng hairy root callus(GHC) cultured with the 1/2MS medium supplemented with 2 mg BA/L was selected for best suspension cultures. The optimum concentration of BA for ginsenosides production was found to be 2 mg/L. Probably the inoculum size of callus plays a role with the ginsenoside production in suspension culture. AS for inoculum size of callus, 50 mg was superior to 150 mg for growth and ginsenoside production. Ginsenoside contents were highest in the suspension culture grown for four weeks under continuous light condition. In fact that continous light treatment promote strongly the synthesis of ginsenoside of the hairy root callus is first result in the world and the numerously induced root hairs of the callus leads a new method for ginsenoside production.
Keywords
hairy root callus; 1/2MS; benzyladenine; ginsenoside; light;
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