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http://dx.doi.org/10.5142/JGR.2003.27.1.001

Effects of Pretreated Korean Red Ginseng on Carbon Tetrachloride and Galactosamine-induced Hepatotoxicity in Rats  

Lee, Chung-Kyu (College of Pharmacy, Kyungsung University)
Kim, Na-Young (Dept. of Food and Nutrition, Dong-A University)
Han, Yong-Nam (Natural Products Research Institute, Seoul National University)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Publication Information
Journal of Ginseng Research / v.27, no.1, 2003 , pp. 1-10 More about this Journal
Abstract
In present study, we examined whether or not the pretreatment of Korean Red Ginseng (KRG) could protect hepatotoxicity induced by carbon tetrachloride (CCl$_4$) and D-galatosamine (GalN). For this study, we not only tested activity of various plasma enzymes (AST, ALT, SDH, LDH), which are used as indicators of liver disease, but also checked the change of liver components such as lipid, glutathione and cytochromes content, and several liver enzyme activity. Pretreatment of KRG for two weeks significantly reduced the elevated plasma enzyme activities induced by CCl$_4$ and GalN. Pretreatment of KRG also restored the hepatic enzymes, malonedialdehyde formation, and depletion of reduced glutathione content induced by CCl$_4$ and GalN to near normal level. However, ${\gamma}$-glutamylcysteine synthetase activity was lot affected by KRG. These results suggest that KRG shows the hepatoprotective effect by reducing lipid peroxidation, by reducing the activity of free radical generating enzymes, and by preserving the hepatic glutathione.
Keywords
Korean red ginseng; hepatptoxicity; hepatoprotective effects; carbon tetrachloride; D-galactosamine;
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  • Reference
1 Yun (Choi), H. S. and Chang I-M.: Kor. J. Pharmacogn., 8, 125 (1977)
2 Chang, I-M. and Yun (Choi), H. S.: ibid, 9, 139 (1978)
3 Chang, I-M., Ryu, J. C., Park, Y. C., Yun (Choi), H. S. and Yang, K. H.: Drug Chem. Toxicol., 6, 443 (1983).   DOI
4 Chang, I-M., Chang, K. S. and Yun, H. S.: Kor. J. Pharmacogn., 14, 95 (1983)
5 Chang, I-M., Yun, H. S., Kim, Y. S. and Ahn, J. W.: Ann. Report Natural Prod. Res. Inst., Seoul Nat. Univ., 23, 59 (1984)
6 장일무，윤혜숙，양규환: 생약연구소업적집 (서울대학교), 23, 90 (1984)
7 Desplaces, A., Choppi, J., Voge, G. and Trost, W: Arzneim. Forschung., 25, 86 (1975)
8 Choppin, J. and Desplaces, A.: Arzneim. Farschung., 29, 63 (1979)
9 Ramellini, G. and Meldolesi, J.: Arzneim. Farschung., 26, 69 (1976).
10 Bindoli, A., Cavallini, L. and Siliprandi, N.: Biochem. Pharmacol., 26, 2405 (1977)   DOI   ScienceOn
11 Kim, B. S., Kim, H. K, Choi, J. W. and Lee, C. K.: Kor. J. Pharmacogn., 27 (1), 47 (1996)
12 Hahn, D. R.: Proc. 2nd Int,l Ginseng Symp., 135(1978)
13 Song, J. H., Park, M. J., Kim, E. and Kim, Y. C.,; YakhakHoeji, 34 (5), 341 (1990)
14 Chng, I-M., Park, Y. C. and Yun (Choi), H. S.: Korean Biochem. J., 15, 200 (1982)
15 Yang, K. H., Kwon, T.J., Choe, S. Y., Yun, H. S. and Chang, I-M.: Drug Chem. Toxicol., 6, 429 (1983)   DOI
16 Matsuda, H., Samukawa, K. and Kuho, M.: Planta Medica, 57, 52 (1991)   DOI   ScienceOn
17 Reitman, S. and Frankel, S. K.: Amer. J. Clin. Pathol., 28, 56 (1957)
18 Weisner, I. S., Rawnsley, H. M., Brooks, F. P. and Senior, J. R.: Am. J. Dig. Dis. 10, 147 (1965)   DOI   ScienceOn
19 Szasa, F.: Clin. Chem., 15, 124(1969)
20 Kind, P. R. N. and King, E. J.: J. Clin. Pathol., 7, 322 (1954)   DOI
21 Berga, L. and Btoida, D.: Sigma Tech. Bull., 500-8-60 (1960)
22 Ohkawa, H., Ohishi, N. and Yaki, K: Anal. Biochem., 95, 351(1979)   DOI   ScienceOn
23 Richardson, R. J. and Murphy, S. D.: Toxicol. Appl. Pharmacol., 31, 505 (1975)   DOI   ScienceOn
24 Summer, K. H. and Greim, H.: Biochem. Biophys. Res. Commun., 96, 566 (1980)   DOI   ScienceOn
25 Omura, T. and Sato, R.: J. Biol. Chem., 239, 2370 (1964)
26 Habig, W. H., Pabist, M. J. and Jakoby, W. B.:J. Biol. Chem., 249, 7130 (1974)
27 Nash, T.:J. Biol. Chem., 55, 416 (1953)
28 Bidlack, W. R. and Lowry, G. L.: Biochem. Pharmacol., 31, 311 (1982)   DOI   ScienceOn
29 Meister; A. and Richman, P. G.: J. Biol. Chem., 250, 1422 (1975)
30 Mize, C. E. and Langdon, R. G.: Hepatic glutathione reductase.: J. Biol. Chem., 237, 1589 (1962)
31 Decker, K. and Kepper, D.: Galactosamine-induced liver injury in Progress in Liver Disease, Popper, H. and Schaffer, E(eds.), Grune & Stratton, New York, vol. 14, p. 183 (1973)
32 Wang, J. and Wendel, A.: Biochem. Pharmacol., 39, 267 (1968)   DOI   ScienceOn
33 Keppler, D., Lesch, R, Reutter, W. and Decker, K.: Exp. Mol. Pathol., 9, 279 (1968)   DOI   ScienceOn
34 El-Mofty, S. K, Scrutton, M. C., Serroni, A., Nicolini, C. and Farber; J. L.: Am. J. Pathol., 79, 579 (1975)
35 Farber, J, L., Gill, G. and Konishi, Y.: Am. J. Pathol., 72, 53 (1973)
36 Lesch, R., Reutter, W., Keppler, D. and Decker, K.: Exp. Mol. Pathol., 12, 58 (1969)   DOI   ScienceOn
37 Miller, E. C. and Miller, J. A.: Hepatocarcinogenesis by chemicals in Progress in liver disease, Popper, H. and Schaffner; E(eds.), Grune & Stratton, New York, Vol. 5, p. 699 (1972)
38 Zieve, L., Anderson, W Rand Dozeman, R.: J. Lab. Clin. Med., 112, 575 (1988)
39 Ahokas, J. T., Davies, C., Ravenscroft, P. J. and Emmerson, B. T.: Biochem. Pharmacol., 33, 1929 (1984)   DOI   ScienceOn
40 Vengerovskii, A. I., Sedykh, I. M., Novozheeva, T. P. and Saratikov, A. S.: Patol. Fiziol. Eksp. Ter., 2, 37 (1990)
41 Ahokas, J. T., Nichollas, F. A., Ravenscroft, P. J. and Emmerson, B. T.: Biochem. Pharmacol., 34, 2157 (1985)   DOI   ScienceOn
42 Joo, C. N.: Proc. 4th Int,l Ginseng Symp., Korean Ginseng and Tabacco Research Institute, 63 (1984)
43 Ahokas. J. T., Nichollas, F. A., Ravenscroft, P. J. and Emmerson, B. T.: Biochem. Pharmacol., 34, 2157 (1985)   DOI   ScienceOn
44 Klassen, C. D. and Fitzgerraid, T. J.: J. Pharmacol. Exp. Ther., 191, 548 (1974)
45 Lowry, O. H., Rodebrough, N. J. Farr, A. L. and Randall, R. J.: J. Biol. Chem., 193, 265 (1951)
46 Noll, T. and de Groot, H.: Biochem. Biophys, Acta., 795, 356 (1984)   DOI   ScienceOn
47 Dodds, M. G. and Foord, R. D.: Br. J. Pharmacol., 40, 227 (1970)   DOI   ScienceOn
48 Ahokas, J. T., Davies, C, Ravenscroft, P. J. and Emmerson, B. T.: Biochem. Pharmacol., 33, 1929 (1984)   DOI   ScienceOn