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http://dx.doi.org/10.9710/kjm.2015.31.3.233

Molecular Characterization and Expression Analysis of a Toll-like receptor 2/6 gene from Abalone (Haliotis discus hannai)  

Moon, Ji Young (Biotechnology Research Division, NFRDI)
Park, Eun Hee (Biotechnology Research Division, NFRDI)
Kong, Hee Jeong (Biotechnology Research Division, NFRDI)
Kim, Young-Ok (Biotechnology Research Division, NFRDI)
Kim, Dong-Gyun (Biotechnology Research Division, NFRDI)
An, Cheul Min (Biotechnology Research Division, NFRDI)
Nam, Bo-Hye (Biotechnology Research Division, NFRDI)
Publication Information
The Korean Journal of Malacology / v.31, no.3, 2015 , pp. 233-241 More about this Journal
Abstract
Toll-like receptors (TLRs) are a major pattern recognition receptor that recognize the structure of invading pathogen and play key roles by triggering immune response. In this study, we identified a sequence of TLR homolog and characterized at molecular level from the abalone (Haliotis discus hannai). Multiple alignments and phylogenetic analysis of abalone TLR protein belongs to the TLR 2/6. Expression level of abalone TLR 2/6 in the tissue was comparatively high in the mantle, gill, digestive duct, and hemocytes, but lowest in the muscle. Expression level of abalone TLR 2/6 mRNA in the mantle, gill, digestive duct, and hemocytes was 20-fold, 60-fold, 115-fold, 112-fold higher than in the muscle, respectively. Expression level of abalone TLR 2/6 mRNA in the mantle was steadily increased until 12 h and decreased post-infection with Vibrio parahemolyticus. While the expression level of abalone TLR 2/6 mRNA in the gill and hemocytes was drastically increased at 6 and 9 h post-infection with Vibrio parahemolyticus, respectively. These results suggest that abalone TLR 2/6 is conserved through evolution and may play roles similar to its mammalian counterparts.
Keywords
Haliotis discus hannai; pattern recognition receptor; Toll-like receptor; Vibrio parahemolyticus;
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