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http://dx.doi.org/10.9710/kjm.2014.30.4.383

Microsatellite multiplex PCR method for selective breeding studies in Pacific abalone (Haliotis discus hannai)  

Park, Choul Ji (Genetics and Breeding Research Center, NFRDI)
Nam, Won Shik (Genetics and Breeding Research Center, NFRDI)
Lee, Myeong Seok (Genetics and Breeding Research Center, NFRDI)
Kang, Ji-Yun (Genetics and Breeding Research Center, NFRDI)
Kim, Kyung Kil (Genetics and Breeding Research Center, NFRDI)
Publication Information
The Korean Journal of Malacology / v.30, no.4, 2014 , pp. 383-390 More about this Journal
Abstract
The multiplex PCR system including six microsatellites from Haliotis discus hannai, consisting of dinucleotide and trinucleotide repeat units, is developed. The six loci were coamplified in a single reaction employing dye-labeled primers. Alleles from these loci were sized using an internal standard by automated sample processing in an ABI3100 Genetic Analyser. Amplified alleles in profiles containing selected microsatellites were typed clearly, providing easily interpretable results. In this results suggest that the presented multiplex PCR system may be a useful tool in a selective breeding program of H. discus hannai in which genetic identification will allow different genotypes to be reared together from fertilization. This should have a great impact as it will make selective breeding more efficient. Moreover, it will be useful in a variety of applications, including strain and hybrid identification, parentage assignment, pedigree reconstruction, estimating genetic diversity and/or inbreeding.
Keywords
Abalone; Selective breeding; Microsatellite DNA marker; Multiplex PCR;
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