Browse > Article
http://dx.doi.org/10.9710/kjm.2014.30.1.41

Changes of enzyme activity in the hemolymph and hepatopancreas of the abalone, Haliotis discus hannai (Ino, 1953) exposed to cadmium  

Min, Eun-Young (Department of Aquatic Life Medicine, Pukyong National University)
Lee, Jung Sick (Department of Aqualife Medicine, Chonnam National University)
Kwak, Ihn-Sil (Faculty of Marine Technology, Chonnam National University)
Kim, Jae Won (Department of Marine Life-Science, Gangwon Provincial College)
Kang, Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
The Korean Journal of Malacology / v.30, no.1, 2014 , pp. 41-49 More about this Journal
Abstract
This study was conducted to investigate the effects of cadmium (Cd) exposure on biochemical factors in the hemolymph and hepatopancreas of the abalone, Haliotis discus hannai. The abalone were exposed to 0, 5, 10, 20 and 40 ${\mu}g/L$ Cd for 4 weeks. The phenoloxidase (PO) activity was decreased in hemolymph of abalone exposed to 40 Cd ${\mu}g/L$ for 4 weeks compared to the control (P < 0.05). The hemolymph enzymes, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were markedly elevated in 40 Cd ${\mu}g/L$ after 4 weeks. The hemolymph calcium concentrations were significantly decreased in 20 and 40 Cd ${\mu}g/L$ for 4 weeks. Hepatopancreas superoxide dismutase (SOD) and catalase (CAT) activities were significantly increased by Cd. SOD was increased in both 20 and 40 Cd ${\mu}g/L$ and CAT, in 40 Cd ${\mu}g/L$ after 2 weeks (P < 0.05). These results suggested that the abalone SOD and CAT including PO may serve as a protective mechanism against oxidative stress by Cd. We conclude that a Cd concentration, 40 ${\mu}g/L$ in water may curtail hemolymph homeostasis and anti-oxidative reactions in abalone hepatopancreas. From these results, these biochemical factors may represent a convenient method of monitoring heavy metal pollution in coastal areas.
Keywords
abalone; Cd; hemolymph; hepatopancreas; PO; SOD; CAT;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Lopes, P.A., Pinheiro, T., Santos, M.C., da Luz Mathias, M., Collares-Pereira, M.J. and Viegas-Crespo, A.M. (2001) Response of antioxidant enzymes in freshwater fish population (Leuciscus alburnoides complex) to inorganic pollutants exposure. Science of the Total Environment, 280: 153-163.   DOI   ScienceOn
2 Mok, J.S., Lee, K.J., Shim, K.B., Lee, T.S., Song, K.C. and Kim, J.H. (2010) Contents of heavy metals in marine invertebrates from the Korean coast. Journal of the Korean Society of Food Science and Nutrition, 39: 894-901.   과학기술학회마을   DOI
3 Nebot, C., Moutet, M., Huet, P., Xu, J.Z., Yadan, J.C. and Chaudiere, J. (1993) Spectrophotometric assay of superoxide dismutase activity based on the activated autoxidation of a tetracyclic catechol. Analytical Biochemistry, 214: 442-451.   DOI   ScienceOn
4 Parihar, M.S., Javeri, T., Hemnani, T., Dubey, A.K. and Parkash, P. (1997) Responses of superoxide dismutase, glutathione peroxidase and reduced glutathione antioxidant defenses in gills of the freshwater catfish (Heteropneustes fossilis) to short-term elevated temperature. Journal of Thermal Biology, 22: 151-156.   DOI   ScienceOn
5 Park, H.J. and Kang, J.C. (2012) Biochemical changes in the hemolymph and hepatopancreas of abalone Haliotis discus hannai exposed to copper. Korean Journal of Fisheries and Aquatic Sciences, 45: 154-160.   과학기술학회마을   DOI   ScienceOn
6 Pipe, R.K., Coles, J.A., Carissan, F.M.M. and Ramanathan, K. (1999) Copper induced immunomodulation in the marine mussel, Mytilus edulis. Aquatic Toxicology, 46: 43-54.   DOI   ScienceOn
7 Shen, H., Zhang, Q., Xu, R. and Wang, G. (1997) Effects of petroleum on the sero-proteins of Tilapia mossambica. Marine Environmental Science, 16: 1-5.
8 Shin, Y.K., Jun, J.C., Im, J.H., Kim, D.W., Son, M.H. and Kim, E.O. (2011) Physiological response in abalone Haliotis discus hannai with different salinity. Korean Journal of Malacology, 27: 283-289.   DOI   ScienceOn
9 Stohs, S.J., Bagchi, D., Hassoun, E. and Bagchi, M. (2000) Oxidative mechanism in the toxicity of chromium and cadmiumions. Journal of environmental Pathology Toxicology and Oncology, 19: 201-213.
10 Sugiyama, M. (1994) Role of cellular antioxidant in metal-induced damage. Cell Biology and Toxicology, 10: 1-22.
11 Wo, K.T., Lam, P.K.S. and Wu, R.S.S. (1999) A comparison of growth biomarkers for assessing sublethal effects of cadmium on a marine gastropod, Nassarius festivus. Marine Pollution Bulletin, 39: 165-173.   DOI   ScienceOn
12 Wang, W., Mai, K., Zhang, W., Ai, Q., Yao, C., Li, H. and Liufu, Z. (2009) Effects of dietary copper on survival, growth and immune response of juvenile abalone, Haliotis discus hannai Ino. Aquaculture, 297: 122-127.   DOI   ScienceOn
13 Wendel, A. and Feuerstein, S. (1981) Drug-induced lipid peroxidation in mice-I Modulation by monooxegenase activity, glutathione and selenium status. Biochemical Pharmacology, 30: 2513-2520.   DOI   ScienceOn
14 Winston, G.W. and Di Giulio, R.T. (1991) Prooxidant and antioxidant mechanisms in aquatic organisms. Aquatic Toxicology, 19: 137-161.   DOI   ScienceOn
15 Blasco, J. and Puppo, J. (1999) Effect of heavy metals (Cu, Cd and Pb) on aspartate and alanine aminotransferase in Ruditapes philippinarum (Mollusca: Bivalvia). Comparative biochemistry and physiology Part C: Pharmacology, toxicology & endocrinology, 122: 253-263.   DOI   ScienceOn
16 Boge, G., Bussiere, D. and Peres, G. (1988) Effets du chrome hexavalent sur les fonctions de la bordure en brosse de l'intestin de la truite arc en ciel (Salmo gairdneri R.). Water Research, 22: 441-447.   DOI   ScienceOn
17 Abdel-Moati, M.A.R. (1992) Biochemical and physiological responses of Mytilus edulis to Hg and Pb in the coastal waters of Alexandria region. Proceeding of the FAD-UNEP-IOC workshop on biological effects of pollutants in marine organisms. Athens, Greece, 69: 61-72.
18 Abel, T.H. and Barlocher, F. (1984) Effect of cadmium on aquatic hyphomycetes. Applied and Environmental Microbiology, 48: 245-251.
19 Barton, B.A. (1991) Physiological changes in fish from stress in aquaculture with emphasis in the response and effects of corticosteroids. Annual Review of Fish diseases, 1: 3-26.   DOI
20 Bhattacharyya, M.H., Whelton, B.D., Stern, P.H. and Peterson, D.P. (1988) Cadmium accelerates bone loss in ovariectomized mice and fetal rat limb bones in culture. Proceedings of the National Academy of Sciences of the United States of America, 85: 8761-8765.   DOI   ScienceOn
21 Cheng, T.C. (1981) Bivalves. In: Invertebrate blood cell (ed. by Ratcliffe, N.A., Rowley, A.F.), London Academic Press, London. pp. 233-299.
22 Cong, R., Sun, W., Liu, G., Fan, T., Meng, X., Yang, L. and Zhu, L. (2005) Purification and characterization of phenoloxidase form clam Ruditapes philippinarum. Fish and Shellfish Immunology, 18: 61-70.   DOI   ScienceOn
23 Courtois, E., Marques, M., Barrientos, A., Casado, S. and Lopez-Farre, A. (2003) Lead-induced downregulation of soluble guanylate cyclase in isolated rat aortic segments mediated by reactive oxygen species and cyclooxygenase-2. Journal of the American Society of Nephrology, 14: 1464-1470.   DOI   ScienceOn
24 Huang, X., Ke, C. and Wang, W.X. (2008) Bioaccumulation of silver, cadmium and mercury in the abalone Haliotis diversicolor from water and food source. Aquaculture, 283: 194-202.   DOI   ScienceOn
25 Firat, O., Cogun, H.Y., Aslanyavrusu, S. and Kargin, F. (2009) Antioxidant responses and metal accumulation in tissues of Nile tilapia Oreochromis niloticus under Zn, Cd and Zn + Cd exposure. Journal of Applied Toxicology, 29: 295-301.   DOI   ScienceOn
26 Forbes, V.E. and Depledge, M.H. (1992) Cadmium effects on the carbon and energy balance of mudsnails. Marine Biology, 113: 263-269.
27 Fried, G.H. and Levin, N.L. (1973) Enzymatic activity in hepatopancreas of Nassarius obsoletus. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 45: 153-157.   DOI   ScienceOn
28 Hontela, A., Daniel, C. and Ricard, A.C. (1996) Effects of acute and subacute exposures to cadmium on the interrenal and thyroid function in rainbow trout, Oncorhynchus mykiss. Aquatic Toxicology, 35: 171-182.   DOI   ScienceOn
29 Hu, G.F., Li, Z., Liang, H.W., Wang, C.Z., Wu, Q.C., Luo, X.Z. and Zou, G.W. (2009) Effects of cadmium on SOD and CAT in hepatopancreas, antennary gland and gill of Procambarus clarkii. Journal of Agro-Environment Science, 28: 1806-1811.
30 Huang, X., Guo, F., Ke, C. and Wang, W.X. (2010) Response of abalone Haliotis diversicolor to sublethal exposure of waterborne and dietary silver and cadmium. Ecotoxicology and Environmental Safety, 73: 1130-1137.   DOI   ScienceOn
31 Johnson, J.K., Rocheleau, T.A., Hillyer, J.F., Chen, C.C., Li, J. and Christensen, B.M. (2003) A potential role for phenylalanine hydroxylase in mosquito immune responses. Insect Biochemistry and Molecular Biology, 33: 345-354.   DOI   ScienceOn
32 Hur, J.W., Chang, Y.J., Lim, H.K. and Lee, B.K. (2001) Stress responses of cultured fishes elicited by water level reduction in rearing tank and fish transference during selection process. Bulletin of the Korean Fisheries Society, 34: 465-472.   과학기술학회마을
33 Kim, T.H., Kim, K.J., Choe, M.K. and Yeo, I.K. (2006) Physiological changes of juvenile abalone, Haliotis sieboldii exposed to acute water temperature stress. Journal of Aquaculture, 19: 77-83.   과학기술학회마을
34 Hwang, U.G., Kim, J.H. and Kang, J.C. (2013) Changes of growth and hematological constituents in the rock bream Oplegnathus fasciatus exposed to TBT. Journal Fish Pathology, 26: 219-229.   과학기술학회마을   DOI   ScienceOn
35 Jee, J.H., Kim, S.G. and Kang, J.C. (2004) Effects of phenanthrene on growth and basic physiological functions of the olive flounder, Paralichthys olivaceus. Journal of Experimental Marine Biology and Ecology, 304: 123-136.   DOI   ScienceOn
36 Kim, T.H., Yang, M.H., Choe, M.K., Han, S.J. and Yeu, I.K. (2005) Physiological studies on acute water-temperature stress of juvenile abalone, Haliotis discus hannai. Journal of Aquaculture, 18: 7-12.
37 KNSO (2011) Korea National Statistical office. Fishery production survey DB, Daejeon, Korea.
38 Koyama, J. and Itazawa, Y. (1977) Effects of oral administration of cadmium on fish: I. Analytical results of the blood and bones. Bulletin of the Japanese Society of Scientific Fishries, 43: 523-526.   DOI
39 Moody, C.S. and Hassan, H.M. (1982) Mutagenicity of oxygen free radicals. Proceedings of the National Academy Sciences, 79: 2855-2859.   DOI   ScienceOn
40 Liu, H., Zhang, J.F., Shen, H., Wang, X.R. and Wang, W.M. (2005) Impact of copper and Its EDTA complex on the glutathione-dependent antioxidant system in freshwater fish (Carassius auratus). Bulletin of Environmental Contamination and Toxicology, 74: 1111-1117.   DOI
41 Park, C.J., Min, B.H., Kim, K.S., Lee, J.W., Lee, J.H., Noh, J.K., Kim, H.C., Park, J.W. and Myeong, J.I. (2011) Physiological responses on low water temperature stress of Pacific abalone, Haliotis discus hannai. Korean Journal of Malacology, 27: 317-322.   과학기술학회마을   DOI   ScienceOn
42 Halliwell, B. and Gutteridge, J.M. (1995) The definition and measurement of antioxidants in biological systems. Free Radical Biology and Medicine, 18: 125-126.   DOI   ScienceOn
43 Ferraris, M., Radice, S., Catalani, P., Francolini, M., Marabini, L. and Chiesara, E. (2002) Early oxidative damage in primary cultured trout hepatocytes: a time course study. Aquatic Toxicology, 59: 283-296.   DOI   ScienceOn