Browse > Article
http://dx.doi.org/10.9710/kjm.2013.29.3.171

Identification, sequence characterization and expression analysis of the arginine kinase gene in response to laminarin challenge from the Oriental land snail, Nesiohelix samarangae  

Jeong, Ji Eun (Department of Life Science, Soonchunhyang University)
Lee, Yong Seok (Department of Life Science, Soonchunhyang University)
Publication Information
The Korean Journal of Malacology / v.29, no.3, 2013 , pp. 171-179 More about this Journal
Abstract
Arginine kinase (ArK) is known to play an important role in most invertebrates the level of ATP by phosphorylation of phosphagens in cell and immuninty in living organisms. ArK has been identified in many kinds of organisms ranging from invertebrate to vertebrate. However, no ArK gene has been cloned and investigated from N. samarangae. This leads us to identify ArK cDNA (NsArK) from the expressed sequence tag (EST) sequencing of N. samarangae. Sequence analysis indicated that the coding region of 1,065 bp contains 355 amino acid residues. Molecular phylogenetic analysis shows that NsArK had very high similarities with mollusca and arthropoda. In an attempt to investigate a potential role of NsArK in the digestive gland of N. samarangae, expression patterns were analyzed. RT-PCR analsysis shows that NsArK mRNA is induced in the rane of 1.2 fold at 6 hr by laminarin when compared with the control. The immunnologial and physiological role of NsArK remains to be further investigated in N. samarangae.
Keywords
cloning; arginine kinase; Nesiohelix samarangae; laminarin;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Adams, R.J., Ewing, J., Dujovny, M., and Misra, M. (1998) Editorial commentary. J. Stroke Cerebrovasc Dis., 7: I-IV.   DOI   ScienceOn
2 Bettencourt, R., Dando, P., Collins, P., Costa, V., Allam, B., and Serrao Santos, R. (2009) Innate immunity in the deep sea hydrothermal vent mussel Bathymodiolus azoricus. Comp. Biochem. Physiol. A. Mol. Integr. Physiol., 152: 278-289.   DOI   ScienceOn
3 Biswas, C., and Mandal, C. (1999) The role of amoebocytes in endotoxin-mediated coagulation in the innate immunity of Achatina fulica snails. Scand. J. Immunol., 49: 131-138.   DOI   ScienceOn
4 Bragg, J., Rajkovic, A., Anderson, C., Curtis, R., Van Houten, J., Begres, B., Naples, C., Snider, M., Fraga, D., and Singer, M. (2012) Identification and characterization of a putative arginine kinase homolog from Myxococcus xanthus required for fruiting body formation and cell differentiation. J. Bacteriol., 194: 2668-2676.   DOI   ScienceOn
5 Braun, R.C., Pedretti, K.T., Casavant, T.L., Scheetz, T.E., Birkett, C.L., and Roberts, C.A. (2001) Parallelization of local BLAST service on workstation clusters. Future Generation Computer Systems, 17: 745-754.   DOI   ScienceOn
6 Buermans, H.P., Ariyurek, Y., van Ommen, G., den Dunnen, J.T., and t Hoen, P.A. (2010) New methods for next generation sequencing based microRNA expression profiling. BMC Genomics, 11: 716.   DOI   ScienceOn
7 Burge, C., and Karlin, S. (1997) Prediction of complete gene structures in human genomic DNA. J. Mol. Biol., 268: 78-94.   DOI   ScienceOn
8 Burge, C.B., and Karlin, S. (1998) Finding the genes in genomic DNA. Curr. Opin. Struct. Biol., 8: 346-354.   DOI   ScienceOn
9 Charlet, M., Chernysh, S., Philippe, H., Hetru, C., Hoffmann, J.A., and Bulet, P. (1996) Innate immunity. Isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusc, Mytilus edulis. J. Biol. Chem., 271: 21808-21813.   DOI   ScienceOn
10 Chistoserdovai, L. (2010) Functional metagenomics: recent advances and future challenges. Biotechnol. Genet. Eng. Rev., 26: 335-352.
11 Coyne, V. (2011) The importance of ATP in the immune system of molluscs. Invertebrate Survival Journal, 8: 48-55.
12 Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res., 32: 1792-1797.   DOI   ScienceOn
13 Cui, S., Zhang, D., Jiang, S., Pu, H., Hu, Y., Guo, H., Chen, M., Su, T., and Zhu, C. (2011) A macrophage migration inhibitory factor like oxidoreductase from pearl oyster Pinctada fucata involved in innate immune responses. Fish Shellfish Immunol, 31: 173-181.   DOI   ScienceOn
14 Dumas, C., and Camonis, J. (1993) Cloning and sequence analysis of the cDNA for arginine kinase of lobster muscle. J. Biol. Chem., 268: 21599-21605.
15 Edgar, R.C. (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics, 5: 113.   DOI   ScienceOn
16 Ewing, B., and Green, P. (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res., 8: 186-194.   DOI
17 Ewing, B., Hillier, L., Wendl, M.C., and Green, P. (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res., 8: 175-185.   DOI   ScienceOn
18 Greenbaum, D., Luscombe, N.M., Jansen, R., Qian, J., and Gerstein, M. (2001) Interrelating different types of genomic data, from proteome to secretome: 'oming in on function. Genome Res., 11: 1463-1468.   DOI   ScienceOn
19 Hegde, P.S., White, I.R., and Debouck, C. (2003) Interplay of transcriptomics and proteomics. Curr. Opin. Biotechnol, 14: 647-651.   DOI   ScienceOn
20 Huang, X., and Madan, A. (1999) CAP3: A DNA sequence assembly program. Genome Res., 9: 868-877.   DOI   ScienceOn
21 Ilg, T., and Werr, M. (2012) Arginine kinase of the sheep blowfly Lucilia cuprina: Gene identification and characterization of the native and recombinant enzyme. Pesticide Biochemistry and Physiology, 102: 115-123.   DOI   ScienceOn
22 Lang, A.B., Wyss, C., and Eppenberger, H.M. (1980) Localization of arginine kinase in muscles fibres of Drosophila melanogaster. J. Muscle Res. Cell. Motil., 1: 147-161.   DOI
23 Iwanaga, S., and Lee, B.L. (2005) Recent advances in the innate immunity of invertebrate animals. J. Biochem. Mol. Biol., 38: 128-150.   DOI   ScienceOn
24 Jarilla, B.R., and Agatsuma, T. (2010) Phosphagen kinases of parasites: unexplored chemotherapeutic targets. Korean J Parasitol, 48: 281-284.   과학기술학회마을   DOI   ScienceOn
25 Jones, D.T., Taylor, W.R., and Thornton, J.M. (1992) The rapid generation of mutation data matrices from protein sequences. Comput. Appl. Biosci., 8: 275-282.
26 Liu, D.W. (2008) Opioid peptides and innate immune response in mollusc. Protein Pept. Lett., 15: 683-686.   DOI   ScienceOn
27 Maeda, M., and Nishizawa, K. (1968) Fine structure of laminaran of Eisenia bicyclis. J. Biochem., 63: 199-206.   DOI
28 McGuffin, L.J., Bryson, K., and Jones, D.T. (2000) The PSIPRED protein structure prediction server. Bioinformatics, 16: 404-405.   DOI   ScienceOn
29 Mitta, G., Vandenbulcke, F., and Roch, P. (2000) Original involvement of antimicrobial peptides in mussel innate immunity. FEBS. Lett., 486: 185-190.   DOI   ScienceOn
30 Newsholme, E.A., Beis, I., Leech, A.R., and Zammit, V.A. (1978) The role of creatine kinase and arginine kinase in muscle. Biochem. J., 172: 533-537.   DOI
31 Reddy, S.R., Roustan, C., and Benyamin, Y. (1991) Purification and properties of two molecular forms of arginine kinase from the adductor muscle of the scallop, Pecten maximus. Comp. Biochem. Physiol. B., 99: 387-394.   DOI   ScienceOn
32 Suzuki, T., and Furukohri, T. (1994) Evolution of phosphagen kinase. Primary structure of glycocyamine kinase and arginine kinase from invertebrates. J. Mol. Biol., 237: 353-357.   DOI   ScienceOn
33 Reddy, S.R., Houmeida, A., Benyamin, Y., and Roustan, C. (1992) Interaction in vitro of scallop muscle arginine kinase with filamentous actin. Eur. J. Biochem., 206: 251-257.   DOI   ScienceOn
34 Rice, P., Longden, I., and Bleasby, A. (2000) EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet., 16: 276-277.   DOI   ScienceOn
35 Supajatura, V., Ushio, H., Nakao, A., Akira, S., Okumura, K., Ra, C., and Ogawa, H. (2002) Differential responses of mast cell Toll-like receptors 2 and 4 in allergy and innate immunity. J. Clin. Invest., 109: 1351-1359.   DOI
36 Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 28: 2731-2739.   DOI   ScienceOn
37 Wang, Z., Jian, J., Lu, Y., Wang, B., and Wu, Z. (2011) A tandem-repeat galectin involved in innate immune response of the pearl oyster Pinctada fucata. Mar. Genomics, 4: 229-236.   DOI   ScienceOn
38 Yu, Z., He, X., Fu, D., and Zhang, Y. (2011) Two superoxide dismutase (SOD) with different subcellular localizations involved in innate immunity in Crassostrea hongkongensis. Fish Shellfish Immunol., 31: 533-539.   DOI   ScienceOn
39 Zhang, H., Wang, L., Song, L., Song, X., Wang, B., Mu, C., and Zhang, Y. (2009) A fibrinogen-related protein from bay scallop Argopecten irradians involved in innate immunity as pattern recognition receptor. Fish Shellfish Immunol., 26: 56-64.   DOI   ScienceOn