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http://dx.doi.org/10.9710/kjm.2013.29.1.7

Changes of Biomarker in Manila clam, Ruditapes philippinarum Exposed to Lead  

Shin, Yun Kyung (Aquaculture Management Division, Aquaculture Research Institute, NFRDI)
Park, Jung Jun (Aquaculture Management Division, Aquaculture Research Institute, NFRDI)
Lim, Hyun Sig (Department of Marine and Fisheries Resources, Mokpo National University)
Lee, Jung Sick (Department of Aqualife Medicine, Chonnam National University)
Publication Information
The Korean Journal of Malacology / v.29, no.1, 2013 , pp. 7-13 More about this Journal
Abstract
This study was conducted to find out biological response of Manila clam, Ruditapes philippinarum exposed to lead (Pb). Experimental period was four weeks. Experimental groups were composed of one control condition and three lead exposure conditions (0.25, 0.50 and 1.00 mg/l). The results of the study confirmed that lead induces reduction of survival rate and oxygen consumption rate and histopathology of organ structure of the bivalve. Oxygen consumption rate was observed exposure groups lower than control decline by 25%-72%. Histological analysis of organ system illustrated expansion of hemolymph sinus, disappearance of epidermal layer and degeneration of connective tissue layer of the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epithelial layer, decrease of mucous cell and degeneration of connective tissue layer in the foot. In the digestive diverticulum, it was showed atrophy of basophilic cell and degeneration of epithelial cell in the digestive tubules, and as the concentration of lead increased the accumulation of lipofuscin increased.
Keywords
Ruditapes philippinarum; Lead (Pb); Survival; Oxygen consumption; Histopathology; Lipofuscin;
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1 Shin, Y.K., Kim, B.H. Oh, B.S., Jung, C.G., Sohn, S.G. and Lee, J.S. (2006) Physiological responses of the ark shell, Scapharca broughtonii (Bivalvia: Arcidae) to decrease in salinity. Journal of Fisheries Science and Technology, 9: 153-159. [in Korean]   DOI   ScienceOn
2 Soto, M., Cajaraville, M.P. and Marigomez, I. (1996) Tissue and cell distribution of copper, zinc and cadmium in the mussel, Mytilus galloprovincialis, determined by autometallography. Tissue and Cell, 28: 557-568.   DOI   ScienceOn
3 Sunila, I. (1988) Acute histological responses of the gill of the mussel, Mytilus edulis, to exposure by environmental pollutants. Journal of Invertebrate Pathology, 52: 137-141.   DOI
4 Vasanthi, L.A., Revathi, P., Arulvasu, C. and Munuswamy, N. (2012) Biomarkers of metal toxicity and histology of Perna viridis from Ennore estuary, Chennai, south east coast of India. Ecotoxicology and Environmental Safety, 84: 92-98.   DOI   ScienceOn
5 Viarengo, A. (1985) Biochemical effects of trace metals. Marine Pollution Bulletin, 16: 153-158.   DOI   ScienceOn
6 Viarengo, A., Canesi, L., Pertica, M., Poli, G., Moore, M.N. and Orunesu, M. (1990) Heavy metal effects on lipid peroxidation in the tissues of Mytilus galloprovincialis Lam. Comparative Biochemistry and Physiology-part C, 97: 37-42.   DOI   ScienceOn
7 Vijayavel, K., Gopalakrishnan, S. and Balasubramanian, M.P. (2007) Sublethal effect of silver and chromium in the green mussel Perna viridis with reference to alterations in oxygen uptake, filtration rate and membrane bound ATPase system as biomarkers. Chemosphere, 69: 979-986.   DOI   ScienceOn
8 Watling, H.R. and Watling, R.J. (1982) Comparative effects of metals on the filtering rate of the brown mussel, Perna perna. Bulletin of Environmental Contamination and Toxicology, 29: 651-657.   DOI   ScienceOn
9 Zorita, I., Ortiz-Zarragoitia, M., Soto, M., Cajaraville, M.P. (2006). Biomarkers in mussels from a copper site gradient (Visnes, Norway): an integrated biochemical, histochemical and histological study. Aquatic Toxicology, 78: S109-S116.   DOI   ScienceOn
10 Krishnakumar, P.K., Asokan, P.K. and Pillai, V.K. (1990) Physiological and cellular responses to copper and mercury in the green mussel Perna viridis (Linnaeus). Aquatic Toxicology, 18: 163-173.   DOI   ScienceOn
11 Lee, J.S. and Park, J.J. (2007) Risk assessment of nonylphenol using the sex ratio, sexual maturation, intersex and lipofuscin accumulation of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). Journal of Korean Fisheries Society, 40: 16-23. [in Korean]   과학기술학회마을   DOI   ScienceOn
12 Marin, M.G., Boscolo, R., Cella, A., Degetto, S. and Ros, L.D. (2006) Field validation of autometallographical black silver deposit (BSD) extent in three bivalve species from the Lagoon of Venice, Italy (Mytilus galloprovincialis, Tapes philippinarum, Scapharca inaequivalvis) for metal bioavailability assessment. Science of Total Environment, 371: 156-167.   DOI   ScienceOn
13 Moore, M.N. (1988) Cytochemical responses of the lysosomal system and NADPH-ferrihemoprotein reductase in molluscan digestive cells to environmental and experimental exposure to xenobiotics. Marine Ecology Progress Series, 46: 81-89.   DOI
14 Moore, M.N. and Allen, J.I. (2002) A computational model of the digestive gland epithelial cell of the marine mussel and its simulated responses to oil-derived aromatic hydrocarbons. Marine Environmental Research, 54: 579-584.   DOI   ScienceOn
15 Morrison, C.M. (1993) Histology and cell ultrastructure of the mantle and mantle lobes of the eastern oyster, Crassostrea virginica, Gmelin: A summary atlas. American Malacological Bulletin, 10: 1-24.
16 NRC (National Research Council). (1983) Risk assessment in the federal government: managing the process. pp. 1-192. National Academy Press, Washington, D.C.
17 Reeve, M.R., Gamble, J.C. and Walter, M.C. (1977) Experimental observation on the effects of cooper on copepod and other zooplankton: controlled ecosystem pollution experiment. Bulletin of Marine Science, 27: 92-104.
18 Otludil, B., Cengiz, E.I., Yildirim, M.Z., Unver, O. and Unlu, E. (2004) The effects of endosulfan on the great ramshorn snail, Planorbariua corneus (Gastropoda, Pulmonata): a histopathological study. Chemosphere, 56: 707-716.   DOI   ScienceOn
19 Park, J.J. and Lee, J.S. (2008) Tributyltin chloride (TBTCI) toxicity on the growth and mantle structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). The Korean Journal of Malacology, 24: 229-241. [in Korean]   과학기술학회마을
20 Park, J.S. and Lee, J.S. (2011) Change of reproductive and histological biomarkers of Ruditapes philippinarum (Bivalvia: Veneridae) exposed to nonylphenol. The Korean Journal of Malacology, 27: 181-190. [in Korean]   과학기술학회마을   DOI   ScienceOn
21 Regoli, F. (1992) Lysosomal responses as a sensitive stress index in biomonitoring heavy metal pollution. Marine Ecology Progress Series, 84: 63-69.   DOI
22 Schintu, M., Durante, L., Maccioni, A., Meloni, P., Degetto, S., and Contu, A. (2008) Measurement of environmental trace-metal levels in mediterranean coastal areas with transplanted mussels and DGT techniques. Marine Pollution Bulletin, 57: 832-837.   DOI   ScienceOn
23 Cooper, N.L., Bidwell, J.R. and Kumar, A. (2009) Toxicity of copper, lead, and zinc mixtures to Ceriodaphnia dubia and Daphnia carinata. Ecotoxicology and Environmental Safety, 72: 1523-1528.   DOI   ScienceOn
24 Da Ros, L., Nasci, C., Marigomez, I. and Soto, M. (2000) Biomarkers and trace metals in the digestive gland of indigenous and transplanted mussels, Mytilus galloprovincialis, in Venice Lagoon, Italy. Marine Environmental Research, 50: 417-423.   DOI   ScienceOn
25 Fichet, D., Radenac, G. and Miramand, P. (1998) Experimental studies of impacts of harbour sediments resuspension to marine invertebrates larvae: Bioavailability of Cd, Cu, Pb and Zn and toxicity. Marine Pollution Bulletin, 36: 509-518.   DOI   ScienceOn
26 Depledge, M.H. and Hopkin, S.P. (1995) Methods to assess effects on brackish, estuarine and near-coastal water organisms. In: Methods to assess the effects of chemicals on ecosystems. (ed. by Linthurst, R.A., Bourdeau, P. and Tardiff, R.G.). pp. 125-149. Wiley, Chichester, UK.
27 Domouhtsidou, G.P. and Dimitriadis, V.K. (2001) Lysosomal and lipid alterations in the digestive gland of mussels, Mytilus galloprovincialis (L.) as biomarkers of environmental stress. Environmental Pollution, 115: 123-137.   DOI   ScienceOn
28 Eble, A.F. (2001) Anatomy and histology of Mercenaria mercenaria. In: Biology of the hard clam. (ed. by Kraeuter, J.N., Castagna, M.). pp. 117-220. Elsevier, New York.
29 Gregory, M.A., George, R.C., Marshall, D.J., Anandraj, A. and Mcclurg, T.P. (1999) The effects of mercury exposure on the surface morphology of gill filament in Perna perna (Mollusca: Bivalvia). Marine Pollution Bulletin, 39: 116-121.   DOI   ScienceOn
30 Hebel, D.K., Jones, M.B. and Depledge, M.H. (1997) Responses of crustaceans to contaminant exposure: a holistic approach. Estuarine, Coastal and Shelf Science, 44: 177-184.   DOI   ScienceOn
31 Huggett, R.J., Kimerle, R.A., Mehrle, P.M. Jr and Bergman, H.L. (1992) Biomarkers: biochemical, physiological, and histological markers of anthropogenic stress. pp. 1-347. Lewis Publishers, Boca Raton, FL.
32 Husmann, G., Abele, D., Monien, D., Monien, P., Kriews, M. and Philipp, E.E.R. (2012) The influence of sedimentation on metal accumulation and cellular oxidative stress markers in the Antarctic bivalve Laternula elliptica. Estuarine Coastal and Shelf Science, 111: 48-59.   DOI   ScienceOn
33 Andhale, A.V., Bhosale, P.A. and Zambare, S.P. (2011) Histopathological study of nickel induced alterations in the fresh water bivalve, Lammellidens marginalis. Journal of Experimental Sciences, 2: 1-3.
34 Ju, S.M., Lee, J.W., Jin, Y.G., Yu, J. and Lee, J.S. (2006) Effect of zinc bioaccumulation on survival rate, activity, growth and organ structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). Journal of Environmental Toxicology, 21: 115-126. [in Korean]   과학기술학회마을
35 Kang, J.C., Kim, H.Y. and Chin, P. (1997) Toxicity of cooper, cadmium and chromium on survival, growth and oxygen consumption of the mysid, Neomysis awatschensis. Journal of Korean Fisheries Society, 30: 874-881. [in Korean]   과학기술학회마을
36 Kim, S.Y. and Lee, T.Y. (1988) The effects of pollutants effluent from a steam-power plant on coastal bivalves. Ocean Research, 10: 47-56. [in Korean]
37 Beninger, P.G., Le Pennec, M. and Salaun, M. (1988) New observations of the gills of Placopecten magellanicus (Mollusca: Bivalvia), and implications for nutrition. Marine Biology, 98: 61-70.   DOI   ScienceOn
38 Brown, B.E. and Newell, R.C. (1972) The effect of copper and zinc on the metabolism of the mussel Mytilus edulis. Marine Biology, 16: 108-118.
39 Byrne, P.A. and O'Halloran, J. (1999) Aspects of assaying sediment toxicity in Irish estuarine ecosystems. Marine Pollution Bulletin, 39: 97-105.   DOI   ScienceOn
40 Cheung, S.G. and Cheung, R.Y. (1995) Effects of heavy metals on oxygen consumption and ammonia excretion in green-lipped mussels (Perna viridis). Marine Pollution Bulletin, 31: 381-386.   DOI   ScienceOn
41 Chin, P., Lee, J.A., Shin, Y.K. and Lee, J.S. (1999) Environmental tolerance for pollutants in Littorina brevicula (Philippi). 2. The growth, metabolism and histological changes exposed to TBTCl and heavy metals in Littorina brevicula. Journal of Korean Fisheries Society, 32: 593-600. [in Korean]   과학기술학회마을