Effect of Diluents on the Cold Storage of Sperm in Scapharca broughtonii (Schrenck)

The effects of diluents composition on cold storage for Scapharca broughtonii (Schrenck) sperm were examined in the percentage of sperm activity and survival rate. Various diluents of glucose solutions (10 mM Hepes-pH 7.8), 600 mM NaCl, stein solution, Ringer's solution (230 mM NaCl, 8 mM KCl, 2 mM $CaCl_2$, 3.7 mM $MgCl_2$, 0.2 mM $NaHCO_3$, 10 mM Hepes-pH 7.8), 20%, 25% ASW (NaCl 2.7 g + KCl 0.07 g + $CaCl_2$ 0.12 g + $MgCl_2$ 0.46 g + $NaHCO_3$ 0.05 g + distilled water 100 ml) were used to store th sperm at $4^{\circ}C$. The storage effect was evaluated using sperm activity and survival rate. Ringer's solution was found to be better diluents which maintained high activity and survival rate of sperm for a storage period of 7 days. Optimal pH of diluents to store the sperm at $4^{\circ}C$ is 7.5.

피조개, Scapharca broughtonii (Schrenck) 정자의 냉장보존에 미치는 희석액의 효과

피조개, Scapharca broughtonii (Schrenck) 정자의 냉장보존을 위해 희석액에 따른 보존효과를 비교하였다. 피조개 정액을 채취하여 600 mM NaCl, Stein solution, 300 mM, 600 mM glucose (10 mM Hepes-pH 7.8), Ringer's solution (230 mM NaCl, 8 mM KCl, 2 mM $CaCl_2$, 3.7 mM $MgCl_2$, 0.2 mM $NaHCO_3$, 10 mM Hepes-pH 7.8), 20%, 25% ASW (NaCl 2.7 g + KCl 0.07 g + $CaCl_2$ 0.12 g + $MgCl_2$ 0.46 g + $NaHCO_3$ 0.05 g + distilled water 100 ml)를 각각 사용하여 $4^{\circ}C$에서 보관하였다. 피조개 정자의 보존효과는 정자의 운동성과 생존율로 평가였다. 피조개 정자는 Ringer's solution에서 7일간 냉장 보전하였을 때 운동성과 생존율을 가장 높았으며, 희석액의 적정 pH는 7.5 이었다.