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Ultrastrucure of Germ Cells during Spermatogenesis and Some Characteristics of Sperm Morphology in Male Mytilus coruscus (Bivalvia: Mytilidae) on the West Coast of Korea  

Kim, Jin-Hee (Korea Inter-University Unstitute of Ocean Science, Pukyong National University)
Chung, Ee-Yung (Korea Marine Environment & Ecosystem Institute, Dive Korea)
Choi, Ki-Ho (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Park, Kwan-Ha (Department of Aquatic Life Medicine, Kunsan National University)
Park, Sung-Woo (Department of Aquatic Life Medicine, Kunsan National University)
Publication Information
The Korean Journal of Malacology / v.26, no.1, 2010 , pp. 33-43 More about this Journal
Abstract
The ultrastructure of germ cells during spermatogenesis and some characteristics of sperm morphology in male Mytilus coruscus, which was collected on the coastal waters of Gyeokpo in western Korea, were investigated by transmission electron microscope observations. The morphology of the spermatozoon has a primitive type and is similar to those of other bivalves in that it contains a short midpiece with five mitochondria surrounding the centrioles. The morphologies of the sperm nucleus type and the acrosome shape of this species have an oval and modified cone shape, respectively. In particular, the axial rod is observed between the nucleus and acrosome of the sperm. The spermatozoon is approximately $45-50{\mu}m$ in length including a sperm nucleus (about $1.46{\mu}m$ in length), an acrosome (about $3.94{\mu}m$ in length) and tail flagellum (approximately $40-45{\mu}m$). The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9+2 structure. Some special charateristics of sperm morphology of this species in the genus Mytilus are (1) acrosomal morphology, (2) the number of mitochondria in the midpiece of the sperm, and (3) the existence of a satellite. The axial rod appears in the acrosome and sperm nucleus as one of the characteristics seen in several species of the subclass Pteriomorphia, unlikely the subclass Heterodonta containing axial filament instead of the axial rod. The number of mitochondria in the midpiece of the sperm of this species in the family Mytilidae are five, as one of common characteristics appeared in most species in the family Mytilidae. Most of Mytilus species contain a satellite body which is attached to the proximal centriole in the middle piece of the sperm, as one of common characteristics of sperm morphology in the family Mytilidae.
Keywords
Mytilus coruscus; spermatogenesis; germ cell; sperm morphology;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 Chung, E.Y., Park, K.Y., and Son, P.W. (2005) Ultrastructural study on spermatogenesis and sexual maturation of the west coast of Korea. Korean Journal of Malacology, 21: 95-105.   과학기술학회마을
2 Chung, E.Y., and Ryou, D.K. (2000) Gametogenesis and sexual maturation of the surf clam Mactra venerifermis on the west coast of Korea. Malacologia, 42: 149-163.
3 Daniels, E.W., Longwell, A.C., McNiff, J.M., and Wolfgang, R.W. (1971) A re-investigation of the ultrastructure of the spermatozoa from the american oyster Crassostrea virginica. Transection American Microscope Society, 90: 275-282.   DOI   ScienceOn
4 Baccetti, B. (1970) the spermatozoon of arthropoda. IX. the sperm cell as an index of arthropod phylogenesis. In: Baccetti B (ed) Comparative spermatology. Accademia Nazionala Dei Lincei, Rome., pp 573
5 Bernard, R.T.F., and Hodgson, A.N. (1985) The fine structure of the sperm and spermatid differentiation in the brown mussel Pernaperna. South Africa Journal of Zoology, 20: 5-9.   DOI
6 Chung, E.Y., Kim, E.J., and Park, G.M. (2007) Spermatogenesis and sexual maturation in male Mactra chinensis (Bivalvia: Mactridae) of Korea. Integrative Biosciences, 11: 227-234.   과학기술학회마을   DOI
7 Chung, E.Y., Kim, H.J., Kim, J.B., and Lee, C.H. (2006) Changes in biochemical components of several tissue in Solen grandis, inrelation to gonad developmental phases. The Korean Journal of Malacology, 22: 27-38.   과학기술학회마을
8 Chung, E.Y., Kim, Y.M., and Lee, S.G. (1999) Ultrastructural study of germ cell development and reproductive cycle of the purplish Washington clam, Saxidomus purpuratus (Sowerby). YellowSea, 5: 51-58.
9 Chung, E.Y., Lee, T.Y., and An, C.M. (1991) Sexual maturation of the venus clam, Cyclina sinensis, on the west coast of Korea. Journal of Medical and Applied Malacology, 3: 125-136.
10 Al-Haji, H.A. (1990) The fine strucyure of spermiogenesis and the mature spermatozoon of Brachiodontes variabilis (Bivalvia). Arab Gulf Journal of Scientific Research (SerB), 8: 153-162.
11 Anderson, W., and Personne, P. (1975) The form and function of spermatozoa: a comparative view. In: Afzelius BA (ed) The functional anatomy of the spermatozoon. Pergamon Press, NewYork, pp 3-14
12 Kim, J.H. (2001) Spermatogenesis and comparative ultrastructure of spermatozoa in several species of Korean economic bivalves (13 families, 34 species). Pukyung National University
13 Pipe, R.K. (1987) Ultrastructural and cytochemical study on interactions between nutrient storage cells and gametogenesis in the mussel Mytilus edulis. Mar. Biol., 96: 919-528.
14 Popham, J.D. (1979) Comparative spermatozoon morphology and bivalve phylogeny. Malacological Review, 12: 1-20.
15 Popham, J.D. (1974) Comparative morphometrics of the acrosomes of the sperms of externally and internally fertilizing sperms of the sperms of the shipworms (Teredinidae, Bivalvia, Mollusca). Cell Tissue Research, 150: 291-297.
16 Sousa, M., and Oliveria, E. (1994) An ultrastructural study of Crassostrea angulata (Mollusca, Bivalvia) spermatogenesis. Marine Biology, 120: 41-47.
17 Jun, J.C., B.S., K., Chung, E.Y., Kim, J.H., Park, G.M., and W., P.S. (2009) Spermatogenesis and ultrastructural characteristics of spermatozoa of brackish water diploid clam, Corbicula japonica (Bivalvia:Corbiculidae). Development and Reproduction, 13: 63-77.   과학기술학회마을
18 Koiki, K. (1985) Comparative ultrastructural studies on the spermatozoa of the Prosobranchia (Mollusca: Gastropoda). . Scientific Report of Faculty of Education, Gunma University, 34: 33-153.
19 Longo, F.G., and Dornfield, E.J. (1967) The fine structure of spermatid differentiation in the mussel, Mytilus edulis. Journal of Ultrastructral Reserch, 20: 462-480.   DOI   ScienceOn
20 Longo, F.J. (1973) An ultrastructural analysis of polysperm in the surf clam, Spisula solidissima. Journal of Experimental Zoology, 183: 153-180.   DOI   ScienceOn
21 Longo, F.J., and Anderson., E. (1969) Spermatogenesis in the surf clam, Spisula solidissima with special reference to the formation of the acrsomal vesicle. Journal of Ultrastructural Research, 27: 435-443.   DOI
22 Nizima, L., and Dan., J.C. (1965) The acrosome reaction in Mytilus edulis. 1. Fine structure of the intact acrosome. Journal of Cell Biology, 25: 243-248.   DOI   ScienceOn
23 Healy, J.M. (1988) Sperm morphology and its systematic importanc in the Gastropoda. In: Ponder WF (ed) Prosobranch phylogeny. Malacological Review, pp 251-266
24 Healy, J.M. (1989) Spermiogenesis and spermatozoa in the relict bivalve genus Neotrigonia: relevance to trigonioid relationships, particularly Unionoidea. Marine Biology, 103: 75-85.   DOI
25 Healy, J.M. (1995) Sperm ultrastructure in the marine bivalve families Carditidae and Crassatellidae and its bearing on unification of the Crasssatelloidea with the Carditoidea. Zoological Science, 24: 21-28.   DOI
26 Healy, J.M. (1996) Molluscan sperm ultrastructure: correlation with taxonomic units with in the Gastropoda, Cephalopoda and Bivalvia. In: Taylor J (ed) Origin and evolutionary radiation of the Mollusca. Oxford University Press, London, pp 99-113.
27 Jamieson, B.G.M. (1991) Fish evolution and systematics: evidence from spermatozoa. Cambridge University Press, Cambridge, pp 181-194
28 Hodgson, A.N., and Bernard, R.T.F. (1986) Ultrastructure of the sperm and spermatogenesis of three species of Mytilidae (Mollusca, Bivalvia). Gamete Research, 15: 123-135.   DOI
29 Humphreys, W.J. (1962) Electron microscope studies on eggs of Mytilus edulis. Journal of Ultrastructural Research, 7: 467-487.   DOI
30 Jamieson, B.G.M. (1987) The ultrastructure and phylogeny of insect spermatozoa. Cambridge University Press, Cambridge
31 Franklin, L.E. (1970) Fertilization and and the role of the acrosome region in non-mammals. Biological Reproduction supplment 2: 159-176.   DOI   ScienceOn
32 Franzen, A. (1956) On spermatogenesis, morphology of the spermatozoon, and biology of fertilization among invertebrates. Zoology of Bidr Uppsala, 31: 355-482.
33 Gaulejac de, J., Jenry, M., and Vicente, N. (1995) An ultrastructural study of gametogenesis of the marine bivalve Pinna nobilis (Linnaeus,1758). II. Spermatogenesis. Journal of Molluscan Study, 61: 393-403.   DOI   ScienceOn
34 Franzen, A. (1970) Phylogenetic aspects of the mophology spermatozoa and spermiogenesis. In: Baccetti B (ed) "Comparative spermatology.". Accademia Naionale Dei Lincei, Rome, pp 573
35 Franzen, A. (1983) Ultrastructural studies of spermatozoa in three bivalve species with notes on evolution of elongated sperm nucleus in primitive spermatozoa. Gamete Research, 7: 199-214.   DOI
36 Galtsoff, F.S., and Phillpott., D.E. (1960) ultrastructure of the spermatozoon of the oyster, Crassostrea virginica. Journal of Ultrastructural Research, 3: 241-253.   DOI   ScienceOn
37 Healy, J.M. (1983) Ultrastructure of euspermatozoa of cerithiacean gastropods (Prosobranchia: Mesogastropoda). Journal of Morphology, 178: 57-75.   DOI
38 Eckelbarger, K.J., and Davis, C.V. (1996) Ultrastructure of the gonad and gametogenesis in the eastern oyster, Crassostrea virginica. II. Testis and spermatogenesis. Marine Biology, 127: 89-96.   DOI   ScienceOn
39 Drozdov, T.A., and Reunov, A.A. (1986) Spermatogenesis and the sperm ultrastructure in the mussel. Modiolus difficillis. Tsitologiia, 28: 1069-1074.
40 Eckelbarger, K.J., Bieler, R., and Mikkelsen, P.M. (1990) ltrastructure of sperm development and mature sperm morphology in three species of commensal bivalves (Mollusca: Galeommatoidea). Journal of Morphology, 205: 63-75.   DOI
41 Dorange, G., and Pennec., M.L. (1989) Ultrastructural characteristics of spermatogenesis in Pecten maximus (Mollusca, Bivalvia). Invertebrate Reproduction & Development 15: 109-117.   DOI
42 Chung, E.Y., and Park, G.M. (1998) Ultrastructural study of spermatogenesis and reproductive cycle of male razor clam, Solen grandis on the west coast of Korea. Development and Reproduction, 2(1): 101-109.