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http://dx.doi.org/10.5850/JKSCT.2013.37.7.852

Immobilization of Trypsin on Chitosan Nonwoven Using Glutaraldehyde  

Kim, Jung Soo (Dept. of Clothing & Textiles, Sookmyung Women's University)
Lee, So Hee (Research Institute of Health and Living Science, Sookmyung Women's University)
Song, Wha Soon (Dept. of Clothing & Textiles, Sookmyung Women's University)
Publication Information
Journal of the Korean Society of Clothing and Textiles / v.37, no.7, 2013 , pp. 852-863 More about this Journal
Abstract
We investigate the immobilization of trypsin on chitosan nonwoven using glutaraldehyde (GA). The conditions for trypsin on chitosan nonwoven and GA cross-linking were optimized depending on different conditions. The order of GA cross-linking was determined by the activity of immobilized trypsin. The characteristics of chitosan nonwoven were examined by Fourier-transform infrared (FT-IR) and surface morphology analyses (SEM). Results showed that the optimal treatment conditions for trypsin on chitosan nonwoven were as follows: pH 8.5; temperature $37^{\circ}C$; trypsin concentration 15% (o.w.f); and treatment time 60 min. Those for GA cross-linking were: pH 10.0; GA concentration 3% (v/v); and treatment time 120 min. FT-IR analysis showed that GA was cross-linked on chitosan nonwoven. The SEM analysis also showed that trypsin was immobilized on chitosan nonwoven.
Keywords
Enzyme; Immobilization; Chitosan; Trypsin; Glutaraldehyde;
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Times Cited By KSCI : 6  (Citation Analysis)
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